Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2154 and Psyr_2155 are separated by 30 nucleotides Psyr_2155 and Psyr_2156 overlap by 4 nucleotides Psyr_2156 and Psyr_2157 are separated by 23 nucleotides
Psyr_2154: Psyr_2154 - transcriptional regulator, LacI family, at 2,510,220 to 2,511,236
_2154
Psyr_2155: Psyr_2155 - Carbohydrate kinase, PfkB, at 2,511,267 to 2,512,187
_2155
Psyr_2156: Psyr_2156 - RbsD or FucU transport, at 2,512,184 to 2,512,588
_2156
Psyr_2157: Psyr_2157 - Inosine/uridine-preferring nucleoside hydrolase, at 2,512,612 to 2,513,637
_2157
Position (kb)
2511
2512
2513 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2510.352 kb on + strand, within Psyr_2154 at 2510.352 kb on + strand, within Psyr_2154 at 2510.353 kb on - strand, within Psyr_2154 at 2510.436 kb on + strand, within Psyr_2154 at 2510.437 kb on - strand, within Psyr_2154 at 2510.539 kb on - strand, within Psyr_2154 at 2510.629 kb on + strand, within Psyr_2154 at 2510.629 kb on + strand, within Psyr_2154 at 2510.630 kb on - strand, within Psyr_2154 at 2510.630 kb on - strand, within Psyr_2154 at 2510.630 kb on - strand, within Psyr_2154 at 2510.633 kb on + strand, within Psyr_2154 at 2510.633 kb on + strand, within Psyr_2154 at 2510.633 kb on + strand, within Psyr_2154 at 2510.633 kb on + strand at 2510.634 kb on - strand, within Psyr_2154 at 2510.712 kb on + strand, within Psyr_2154 at 2510.712 kb on + strand, within Psyr_2154 at 2510.712 kb on + strand, within Psyr_2154 at 2510.712 kb on + strand, within Psyr_2154 at 2510.712 kb on + strand, within Psyr_2154 at 2510.713 kb on - strand, within Psyr_2154 at 2510.763 kb on - strand, within Psyr_2154 at 2511.281 kb on - strand at 2511.286 kb on + strand at 2511.286 kb on + strand at 2511.287 kb on - strand at 2511.287 kb on - strand at 2511.289 kb on + strand at 2511.289 kb on + strand at 2511.289 kb on + strand at 2511.289 kb on + strand at 2511.289 kb on + strand at 2511.290 kb on - strand at 2511.290 kb on - strand at 2511.290 kb on - strand at 2511.290 kb on - strand at 2511.795 kb on + strand, within Psyr_2155 at 2511.796 kb on - strand, within Psyr_2155 at 2511.810 kb on + strand, within Psyr_2155 at 2511.980 kb on + strand, within Psyr_2155 at 2511.981 kb on - strand, within Psyr_2155 at 2511.981 kb on - strand, within Psyr_2155 at 2512.165 kb on + strand at 2512.387 kb on - strand, within Psyr_2156 at 2512.387 kb on - strand, within Psyr_2156 at 2512.550 kb on + strand at 2512.551 kb on - strand at 2512.551 kb on - strand at 2512.551 kb on - strand at 2512.555 kb on + strand at 2512.555 kb on + strand at 2512.556 kb on - strand at 2512.586 kb on + strand at 2512.586 kb on + strand at 2512.586 kb on + strand at 2512.586 kb on + strand at 2512.587 kb on - strand at 2512.589 kb on + strand at 2512.590 kb on - strand at 2512.612 kb on - strand at 2512.612 kb on - strand at 2512.754 kb on + strand, within Psyr_2157 at 2512.755 kb on - strand, within Psyr_2157 at 2512.889 kb on - strand, within Psyr_2157 at 2512.889 kb on - strand, within Psyr_2157 at 2512.927 kb on + strand, within Psyr_2157 at 2512.928 kb on - strand, within Psyr_2157 at 2512.928 kb on - strand, within Psyr_2157 at 2513.053 kb on - strand, within Psyr_2157 at 2513.178 kb on + strand, within Psyr_2157
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 2,510,352 + Psyr_2154 0.13 +1.1 2,510,352 + Psyr_2154 0.13 +1.7 2,510,353 - Psyr_2154 0.13 -2.1 2,510,436 + Psyr_2154 0.21 +1.0 2,510,437 - Psyr_2154 0.21 +1.1 2,510,539 - Psyr_2154 0.31 -2.3 2,510,629 + Psyr_2154 0.40 +2.1 2,510,629 + Psyr_2154 0.40 -0.2 2,510,630 - Psyr_2154 0.40 +1.9 2,510,630 - Psyr_2154 0.40 -1.3 2,510,630 - Psyr_2154 0.40 -0.7 2,510,633 + Psyr_2154 0.41 +0.6 2,510,633 + Psyr_2154 0.41 -0.1 2,510,633 + Psyr_2154 0.41 -0.5 2,510,633 + +0.5 2,510,634 - Psyr_2154 0.41 +1.5 2,510,712 + Psyr_2154 0.48 -0.0 2,510,712 + Psyr_2154 0.48 -0.8 2,510,712 + Psyr_2154 0.48 +0.1 2,510,712 + Psyr_2154 0.48 +0.3 2,510,712 + Psyr_2154 0.48 +0.3 2,510,713 - Psyr_2154 0.48 -1.8 2,510,763 - Psyr_2154 0.53 +0.8 2,511,281 - -1.0 2,511,286 + -0.5 2,511,286 + +0.4 2,511,287 - -0.1 2,511,287 - +0.1 2,511,289 + +0.4 2,511,289 + +1.0 2,511,289 + +2.7 2,511,289 + +0.4 2,511,289 + +0.4 2,511,290 - -1.5 2,511,290 - +0.7 2,511,290 - -0.6 2,511,290 - +0.1 2,511,795 + Psyr_2155 0.57 +1.1 2,511,796 - Psyr_2155 0.57 +0.4 2,511,810 + Psyr_2155 0.59 +0.8 2,511,980 + Psyr_2155 0.77 -1.7 2,511,981 - Psyr_2155 0.78 +0.3 2,511,981 - Psyr_2155 0.78 +0.8 2,512,165 + -0.9 2,512,387 - Psyr_2156 0.50 +0.1 2,512,387 - Psyr_2156 0.50 +1.4 2,512,550 + -1.5 2,512,551 - -0.2 2,512,551 - +1.2 2,512,551 - -0.1 2,512,555 + -2.2 2,512,555 + -0.8 2,512,556 - -1.8 2,512,586 + +0.9 2,512,586 + -1.3 2,512,586 + +1.1 2,512,586 + +0.1 2,512,587 - -0.5 2,512,589 + +0.4 2,512,590 - +1.3 2,512,612 - +0.8 2,512,612 - +0.9 2,512,754 + Psyr_2157 0.14 +1.0 2,512,755 - Psyr_2157 0.14 +0.1 2,512,889 - Psyr_2157 0.27 -1.2 2,512,889 - Psyr_2157 0.27 +1.2 2,512,927 + Psyr_2157 0.31 +0.1 2,512,928 - Psyr_2157 0.31 -0.3 2,512,928 - Psyr_2157 0.31 -1.0 2,513,053 - Psyr_2157 0.43 -0.1 2,513,178 + Psyr_2157 0.55 -1.2
Or see this region's nucleotide sequence