Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2125 and Psyr_2126 overlap by 4 nucleotides Psyr_2126 and Psyr_2127 are separated by 58 nucleotides
Psyr_2125: Psyr_2125 - 3-carboxy-cis,cis-muconate cycloisomerase, at 2,482,758 to 2,484,119
_2125
Psyr_2126: Psyr_2126 - 4-carboxymuconolactone decarboxylase, at 2,484,116 to 2,484,514
_2126
Psyr_2127: Psyr_2127 - outer membrane porin, at 2,484,573 to 2,485,829
_2127
Position (kb)
2484
2485 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2484.055 kb on - strand at 2484.259 kb on + strand, within Psyr_2126 at 2484.260 kb on - strand, within Psyr_2126 at 2484.260 kb on - strand, within Psyr_2126 at 2484.260 kb on - strand, within Psyr_2126 at 2484.425 kb on + strand at 2484.425 kb on + strand, within Psyr_2126 at 2484.426 kb on - strand, within Psyr_2126 at 2484.634 kb on + strand at 2484.635 kb on - strand at 2484.703 kb on + strand, within Psyr_2127 at 2484.703 kb on + strand, within Psyr_2127 at 2484.703 kb on + strand, within Psyr_2127 at 2484.703 kb on + strand, within Psyr_2127 at 2484.703 kb on + strand, within Psyr_2127 at 2484.703 kb on + strand, within Psyr_2127 at 2484.704 kb on - strand, within Psyr_2127 at 2484.704 kb on - strand, within Psyr_2127 at 2484.704 kb on - strand, within Psyr_2127 at 2484.704 kb on - strand, within Psyr_2127 at 2484.778 kb on + strand, within Psyr_2127 at 2484.779 kb on - strand, within Psyr_2127 at 2484.779 kb on - strand, within Psyr_2127 at 2484.826 kb on + strand, within Psyr_2127 at 2484.880 kb on + strand, within Psyr_2127 at 2484.880 kb on + strand, within Psyr_2127 at 2484.880 kb on + strand, within Psyr_2127 at 2484.880 kb on + strand, within Psyr_2127 at 2484.880 kb on + strand, within Psyr_2127 at 2484.881 kb on - strand, within Psyr_2127 at 2484.881 kb on - strand, within Psyr_2127 at 2484.881 kb on - strand, within Psyr_2127 at 2484.965 kb on - strand, within Psyr_2127 at 2485.063 kb on + strand, within Psyr_2127 at 2485.063 kb on + strand, within Psyr_2127 at 2485.063 kb on + strand, within Psyr_2127 at 2485.064 kb on - strand, within Psyr_2127 at 2485.123 kb on + strand, within Psyr_2127 at 2485.123 kb on + strand, within Psyr_2127 at 2485.123 kb on + strand, within Psyr_2127 at 2485.124 kb on - strand, within Psyr_2127 at 2485.135 kb on + strand, within Psyr_2127 at 2485.135 kb on + strand, within Psyr_2127 at 2485.136 kb on - strand, within Psyr_2127 at 2485.195 kb on + strand, within Psyr_2127
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 2,484,055 - -0.0 2,484,259 + Psyr_2126 0.36 +1.1 2,484,260 - Psyr_2126 0.36 +1.1 2,484,260 - Psyr_2126 0.36 +0.7 2,484,260 - Psyr_2126 0.36 +0.8 2,484,425 + -0.9 2,484,425 + Psyr_2126 0.77 +0.7 2,484,426 - Psyr_2126 0.78 -0.4 2,484,634 + +3.4 2,484,635 - -0.9 2,484,703 + Psyr_2127 0.10 -0.5 2,484,703 + Psyr_2127 0.10 -0.4 2,484,703 + Psyr_2127 0.10 +1.1 2,484,703 + Psyr_2127 0.10 -2.2 2,484,703 + Psyr_2127 0.10 -0.0 2,484,703 + Psyr_2127 0.10 +0.1 2,484,704 - Psyr_2127 0.10 -1.0 2,484,704 - Psyr_2127 0.10 +3.1 2,484,704 - Psyr_2127 0.10 +1.2 2,484,704 - Psyr_2127 0.10 +1.0 2,484,778 + Psyr_2127 0.16 -1.5 2,484,779 - Psyr_2127 0.16 -0.3 2,484,779 - Psyr_2127 0.16 +1.1 2,484,826 + Psyr_2127 0.20 -0.0 2,484,880 + Psyr_2127 0.24 -1.2 2,484,880 + Psyr_2127 0.24 -1.4 2,484,880 + Psyr_2127 0.24 +0.6 2,484,880 + Psyr_2127 0.24 -2.1 2,484,880 + Psyr_2127 0.24 +0.8 2,484,881 - Psyr_2127 0.25 +1.0 2,484,881 - Psyr_2127 0.25 +0.5 2,484,881 - Psyr_2127 0.25 -0.6 2,484,965 - Psyr_2127 0.31 +1.7 2,485,063 + Psyr_2127 0.39 -1.5 2,485,063 + Psyr_2127 0.39 -0.5 2,485,063 + Psyr_2127 0.39 +0.7 2,485,064 - Psyr_2127 0.39 -0.1 2,485,123 + Psyr_2127 0.44 +0.2 2,485,123 + Psyr_2127 0.44 +0.3 2,485,123 + Psyr_2127 0.44 +1.1 2,485,124 - Psyr_2127 0.44 -0.6 2,485,135 + Psyr_2127 0.45 -1.5 2,485,135 + Psyr_2127 0.45 +1.1 2,485,136 - Psyr_2127 0.45 -0.5 2,485,195 + Psyr_2127 0.49 +0.7
Or see this region's nucleotide sequence