Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2121

Experiment: KB with Ginkgolide A 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2119 and Psyr_2120 are separated by 57 nucleotidesPsyr_2120 and Psyr_2121 are separated by 52 nucleotidesPsyr_2121 and Psyr_2122 are separated by 141 nucleotidesPsyr_2122 and Psyr_2123 are separated by 11 nucleotides Psyr_2119: Psyr_2119 - Acyltransferase 3, at 2,477,478 to 2,478,548 _2119 Psyr_2120: Psyr_2120 - conserved hypothetical protein, at 2,478,606 to 2,478,848 _2120 Psyr_2121: Psyr_2121 - regulatory protein, LysR:LysR, substrate-binding protein, at 2,478,901 to 2,479,821 _2121 Psyr_2122: Psyr_2122 - protocatechuate 3,4-dioxygenase, beta subunit, at 2,479,963 to 2,480,682 _2122 Psyr_2123: Psyr_2123 - protocatechuate 3,4-dioxygenase, alpha subunit, at 2,480,694 to 2,481,296 _2123 Position (kb) 2478 2479 2480Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2477.904 kb on + strand, within Psyr_2119at 2477.904 kb on + strand, within Psyr_2119at 2477.905 kb on - strand, within Psyr_2119at 2477.905 kb on - strand, within Psyr_2119at 2477.905 kb on - strand, within Psyr_2119at 2477.905 kb on - strand, within Psyr_2119at 2477.994 kb on - strand, within Psyr_2119at 2478.015 kb on + strand, within Psyr_2119at 2478.015 kb on + strand, within Psyr_2119at 2478.016 kb on - strand, within Psyr_2119at 2478.016 kb on - strand, within Psyr_2119at 2478.093 kb on + strand, within Psyr_2119at 2478.192 kb on + strand, within Psyr_2119at 2478.192 kb on + strand, within Psyr_2119at 2478.192 kb on + strand, within Psyr_2119at 2478.193 kb on - strand, within Psyr_2119at 2478.193 kb on - strand, within Psyr_2119at 2478.193 kb on - strand, within Psyr_2119at 2478.193 kb on - strand, within Psyr_2119at 2478.321 kb on - strand, within Psyr_2119at 2478.327 kb on + strand, within Psyr_2119at 2478.328 kb on - strand, within Psyr_2119at 2478.336 kb on + strand, within Psyr_2119at 2478.336 kb on + strand, within Psyr_2119at 2478.336 kb on + strand, within Psyr_2119at 2478.336 kb on + strand, within Psyr_2119at 2478.336 kb on + strand, within Psyr_2119at 2478.336 kb on + strand, within Psyr_2119at 2478.336 kb on + strand, within Psyr_2119at 2478.336 kb on + strand, within Psyr_2119at 2478.336 kb on + strand, within Psyr_2119at 2478.337 kb on - strand, within Psyr_2119at 2478.337 kb on - strand, within Psyr_2119at 2478.337 kb on - strand, within Psyr_2119at 2478.337 kb on - strand, within Psyr_2119at 2478.337 kb on - strand, within Psyr_2119at 2478.337 kb on - strand, within Psyr_2119at 2478.337 kb on - strand, within Psyr_2119at 2478.337 kb on - strand, within Psyr_2119at 2478.337 kb on - strand, within Psyr_2119at 2478.408 kb on + strand, within Psyr_2119at 2478.409 kb on - strand, within Psyr_2119at 2478.469 kb on + strandat 2478.480 kb on + strandat 2478.480 kb on + strandat 2478.569 kb on - strandat 2479.205 kb on + strand, within Psyr_2121at 2479.205 kb on + strand, within Psyr_2121at 2479.306 kb on + strand, within Psyr_2121at 2479.307 kb on - strand, within Psyr_2121at 2479.328 kb on + strand, within Psyr_2121at 2479.402 kb on + strand, within Psyr_2121at 2479.402 kb on + strand, within Psyr_2121at 2479.403 kb on - strand, within Psyr_2121at 2479.403 kb on - strand, within Psyr_2121at 2479.938 kb on - strandat 2479.938 kb on - strandat 2480.035 kb on + strand, within Psyr_2122at 2480.035 kb on + strand, within Psyr_2122at 2480.035 kb on + strand, within Psyr_2122at 2480.197 kb on - strand, within Psyr_2122at 2480.197 kb on - strand, within Psyr_2122at 2480.228 kb on - strand, within Psyr_2122at 2480.308 kb on + strand, within Psyr_2122at 2480.309 kb on - strand, within Psyr_2122at 2480.375 kb on - strand, within Psyr_2122at 2480.375 kb on - strand, within Psyr_2122at 2480.405 kb on - strand, within Psyr_2122at 2480.500 kb on + strand, within Psyr_2122at 2480.500 kb on + strand, within Psyr_2122

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM
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2,477,904 + Psyr_2119 0.40 +0.1
2,477,904 + Psyr_2119 0.40 -0.4
2,477,905 - Psyr_2119 0.40 +1.5
2,477,905 - Psyr_2119 0.40 +4.1
2,477,905 - Psyr_2119 0.40 +0.1
2,477,905 - Psyr_2119 0.40 -0.3
2,477,994 - Psyr_2119 0.48 -0.3
2,478,015 + Psyr_2119 0.50 -0.2
2,478,015 + Psyr_2119 0.50 +2.4
2,478,016 - Psyr_2119 0.50 +0.7
2,478,016 - Psyr_2119 0.50 +0.7
2,478,093 + Psyr_2119 0.57 +1.1
2,478,192 + Psyr_2119 0.67 -1.5
2,478,192 + Psyr_2119 0.67 -0.1
2,478,192 + Psyr_2119 0.67 +0.2
2,478,193 - Psyr_2119 0.67 +0.3
2,478,193 - Psyr_2119 0.67 +0.7
2,478,193 - Psyr_2119 0.67 -0.6
2,478,193 - Psyr_2119 0.67 +0.7
2,478,321 - Psyr_2119 0.79 +0.1
2,478,327 + Psyr_2119 0.79 +3.4
2,478,328 - Psyr_2119 0.79 -0.4
2,478,336 + Psyr_2119 0.80 -2.0
2,478,336 + Psyr_2119 0.80 +1.1
2,478,336 + Psyr_2119 0.80 +3.2
2,478,336 + Psyr_2119 0.80 -0.1
2,478,336 + Psyr_2119 0.80 +1.1
2,478,336 + Psyr_2119 0.80 -1.2
2,478,336 + Psyr_2119 0.80 -1.5
2,478,336 + Psyr_2119 0.80 -0.1
2,478,336 + Psyr_2119 0.80 +1.4
2,478,337 - Psyr_2119 0.80 +0.1
2,478,337 - Psyr_2119 0.80 -2.8
2,478,337 - Psyr_2119 0.80 -1.4
2,478,337 - Psyr_2119 0.80 +1.4
2,478,337 - Psyr_2119 0.80 +0.1
2,478,337 - Psyr_2119 0.80 -1.7
2,478,337 - Psyr_2119 0.80 +1.8
2,478,337 - Psyr_2119 0.80 -1.0
2,478,337 - Psyr_2119 0.80 -0.8
2,478,408 + Psyr_2119 0.87 -1.5
2,478,409 - Psyr_2119 0.87 -0.4
2,478,469 + -1.2
2,478,480 + +0.3
2,478,480 + -1.9
2,478,569 - -0.7
2,479,205 + Psyr_2121 0.33 +0.6
2,479,205 + Psyr_2121 0.33 +1.1
2,479,306 + Psyr_2121 0.44 +0.5
2,479,307 - Psyr_2121 0.44 +0.9
2,479,328 + Psyr_2121 0.46 +0.8
2,479,402 + Psyr_2121 0.54 -0.6
2,479,402 + Psyr_2121 0.54 -1.9
2,479,403 - Psyr_2121 0.55 +0.2
2,479,403 - Psyr_2121 0.55 +1.0
2,479,938 - +3.1
2,479,938 - +0.5
2,480,035 + Psyr_2122 0.10 +0.8
2,480,035 + Psyr_2122 0.10 +0.8
2,480,035 + Psyr_2122 0.10 -1.8
2,480,197 - Psyr_2122 0.33 -0.6
2,480,197 - Psyr_2122 0.33 -0.1
2,480,228 - Psyr_2122 0.37 +1.2
2,480,308 + Psyr_2122 0.48 +0.4
2,480,309 - Psyr_2122 0.48 +0.3
2,480,375 - Psyr_2122 0.57 +0.3
2,480,375 - Psyr_2122 0.57 +0.1
2,480,405 - Psyr_2122 0.61 +2.7
2,480,500 + Psyr_2122 0.75 -0.3
2,480,500 + Psyr_2122 0.75 +1.2

Or see this region's nucleotide sequence