Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2037 and Psyr_2038 are separated by 37 nucleotides Psyr_2038 and Psyr_2039 are separated by 107 nucleotides Psyr_2039 and Psyr_2040 are separated by 361 nucleotides
Psyr_2037: Psyr_2037 - Fimbrial biogenesis outer membrane usher protein, at 2,364,893 to 2,367,406
_2037
Psyr_2038: Psyr_2038 - pili assembly chaperone:Bacterial pili assembly chaperone, at 2,367,444 to 2,368,190
_2038
Psyr_2039: Psyr_2039 - Fimbrial protein, at 2,368,298 to 2,368,849
_2039
Psyr_2040: Psyr_2040 - YD repeat protein, at 2,369,211 to 2,374,259
_2040
Position (kb)
2368
2369 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2367.359 kb on - strand at 2367.359 kb on - strand at 2367.399 kb on - strand at 2367.399 kb on - strand at 2367.464 kb on + strand at 2367.464 kb on + strand at 2367.464 kb on + strand at 2367.464 kb on + strand at 2367.464 kb on + strand at 2367.464 kb on + strand at 2367.465 kb on - strand at 2367.465 kb on - strand at 2367.740 kb on + strand, within Psyr_2038 at 2367.741 kb on - strand, within Psyr_2038 at 2367.763 kb on + strand, within Psyr_2038 at 2367.795 kb on - strand, within Psyr_2038 at 2367.801 kb on + strand, within Psyr_2038 at 2367.850 kb on + strand, within Psyr_2038 at 2367.851 kb on - strand, within Psyr_2038 at 2367.903 kb on + strand, within Psyr_2038 at 2367.903 kb on + strand, within Psyr_2038 at 2367.989 kb on + strand, within Psyr_2038 at 2368.049 kb on + strand, within Psyr_2038 at 2368.049 kb on + strand, within Psyr_2038 at 2368.050 kb on - strand, within Psyr_2038 at 2368.050 kb on - strand, within Psyr_2038 at 2368.116 kb on + strand at 2368.116 kb on + strand at 2368.117 kb on - strand at 2368.117 kb on - strand at 2368.117 kb on - strand at 2368.118 kb on + strand at 2368.118 kb on + strand at 2368.119 kb on - strand at 2368.119 kb on - strand at 2368.119 kb on - strand at 2368.119 kb on - strand at 2368.119 kb on - strand at 2368.119 kb on - strand at 2368.120 kb on + strand at 2368.120 kb on + strand at 2368.120 kb on + strand at 2368.120 kb on + strand at 2368.120 kb on + strand at 2368.120 kb on + strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.121 kb on - strand at 2368.198 kb on + strand at 2368.205 kb on + strand at 2368.205 kb on + strand at 2368.206 kb on - strand at 2368.234 kb on + strand at 2368.262 kb on - strand at 2368.271 kb on + strand at 2368.296 kb on + strand at 2368.296 kb on + strand at 2368.296 kb on + strand at 2368.297 kb on - strand at 2368.297 kb on - strand at 2368.322 kb on + strand at 2368.322 kb on + strand at 2368.322 kb on + strand at 2368.322 kb on + strand at 2368.322 kb on + strand at 2368.323 kb on - strand at 2368.323 kb on - strand at 2368.323 kb on - strand at 2368.323 kb on - strand at 2368.323 kb on - strand at 2368.371 kb on + strand, within Psyr_2039 at 2368.375 kb on - strand, within Psyr_2039 at 2368.420 kb on - strand, within Psyr_2039 at 2368.725 kb on - strand, within Psyr_2039 at 2368.816 kb on + strand at 2368.870 kb on + strand at 2368.887 kb on + strand at 2368.887 kb on + strand at 2368.899 kb on + strand at 2368.899 kb on + strand at 2368.903 kb on + strand at 2368.969 kb on + strand at 2369.018 kb on + strand at 2369.019 kb on - strand at 2369.038 kb on + strand at 2369.059 kb on + strand at 2369.130 kb on - strand at 2369.209 kb on + strand at 2369.227 kb on + strand at 2369.227 kb on + strand at 2369.227 kb on + strand at 2369.227 kb on + strand at 2369.227 kb on + strand at 2369.227 kb on + strand at 2369.227 kb on + strand at 2369.228 kb on - strand at 2369.228 kb on - strand at 2369.228 kb on - strand at 2369.228 kb on - strand at 2369.228 kb on - strand at 2369.228 kb on - strand at 2369.228 kb on - strand at 2369.228 kb on - strand at 2369.231 kb on + strand at 2369.231 kb on + strand at 2369.231 kb on + strand at 2369.232 kb on - strand at 2369.232 kb on - strand at 2369.232 kb on - strand at 2369.269 kb on - strand at 2369.269 kb on - strand at 2369.390 kb on + strand at 2369.390 kb on + strand at 2369.390 kb on + strand at 2369.390 kb on + strand at 2369.391 kb on - strand at 2369.391 kb on - strand at 2369.391 kb on - strand at 2369.460 kb on - strand at 2369.632 kb on - strand at 2369.647 kb on + strand at 2369.657 kb on - strand at 2369.701 kb on + strand at 2369.701 kb on + strand at 2369.701 kb on + strand at 2369.701 kb on + strand at 2369.701 kb on + strand at 2369.701 kb on + strand at 2369.702 kb on - strand at 2369.702 kb on - strand at 2369.702 kb on - strand at 2369.702 kb on - strand at 2369.702 kb on - strand at 2369.702 kb on - strand at 2369.705 kb on + strand at 2369.705 kb on + strand at 2369.705 kb on + strand at 2369.705 kb on + strand at 2369.706 kb on - strand at 2369.706 kb on - strand at 2369.706 kb on - strand at 2369.706 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 2,367,359 - -0.9 2,367,359 - -2.3 2,367,399 - +1.0 2,367,399 - -0.5 2,367,464 + +0.2 2,367,464 + +3.4 2,367,464 + +0.4 2,367,464 + -0.1 2,367,464 + -0.5 2,367,464 + +0.3 2,367,465 - +0.2 2,367,465 - +0.0 2,367,740 + Psyr_2038 0.40 -0.5 2,367,741 - Psyr_2038 0.40 -0.7 2,367,763 + Psyr_2038 0.43 +1.2 2,367,795 - Psyr_2038 0.47 +0.0 2,367,801 + Psyr_2038 0.48 -0.6 2,367,850 + Psyr_2038 0.54 +0.6 2,367,851 - Psyr_2038 0.54 -1.2 2,367,903 + Psyr_2038 0.61 -1.8 2,367,903 + Psyr_2038 0.61 -0.8 2,367,989 + Psyr_2038 0.73 +1.3 2,368,049 + Psyr_2038 0.81 -0.1 2,368,049 + Psyr_2038 0.81 -0.3 2,368,050 - Psyr_2038 0.81 +0.8 2,368,050 - Psyr_2038 0.81 +0.7 2,368,116 + +1.0 2,368,116 + +1.8 2,368,117 - -0.9 2,368,117 - +0.0 2,368,117 - -3.3 2,368,118 + -0.5 2,368,118 + -1.3 2,368,119 - +1.0 2,368,119 - -1.8 2,368,119 - +1.5 2,368,119 - -0.4 2,368,119 - +0.2 2,368,119 - -0.7 2,368,120 + -1.4 2,368,120 + +0.4 2,368,120 + +0.7 2,368,120 + -0.2 2,368,120 + -0.9 2,368,120 + -1.5 2,368,121 - +0.0 2,368,121 - -0.4 2,368,121 - +2.5 2,368,121 - +0.6 2,368,121 - -0.1 2,368,121 - -3.0 2,368,121 - -1.3 2,368,121 - -1.1 2,368,121 - +1.1 2,368,121 - -1.3 2,368,121 - +0.3 2,368,121 - -0.5 2,368,121 - -1.0 2,368,121 - -0.3 2,368,121 - -0.5 2,368,198 + -0.9 2,368,205 + +0.1 2,368,205 + -0.5 2,368,206 - +0.2 2,368,234 + -0.2 2,368,262 - -1.0 2,368,271 + -1.3 2,368,296 + -0.9 2,368,296 + -0.5 2,368,296 + -0.7 2,368,297 - +0.6 2,368,297 - +0.5 2,368,322 + -1.1 2,368,322 + +0.4 2,368,322 + -0.8 2,368,322 + -0.4 2,368,322 + -2.1 2,368,323 - +0.0 2,368,323 - +2.2 2,368,323 - -0.9 2,368,323 - -1.8 2,368,323 - +0.0 2,368,371 + Psyr_2039 0.13 +1.7 2,368,375 - Psyr_2039 0.14 +0.4 2,368,420 - Psyr_2039 0.22 +3.4 2,368,725 - Psyr_2039 0.77 -2.0 2,368,816 + -0.5 2,368,870 + +1.0 2,368,887 + +0.6 2,368,887 + -0.2 2,368,899 + -0.4 2,368,899 + +1.8 2,368,903 + -0.5 2,368,969 + -1.7 2,369,018 + -1.4 2,369,019 - -0.9 2,369,038 + -0.2 2,369,059 + +0.2 2,369,130 - -0.8 2,369,209 + +0.3 2,369,227 + +2.0 2,369,227 + +0.5 2,369,227 + +2.2 2,369,227 + +2.8 2,369,227 + +0.0 2,369,227 + +0.7 2,369,227 + +0.8 2,369,228 - -0.4 2,369,228 - +0.5 2,369,228 - -0.8 2,369,228 - +0.2 2,369,228 - +0.3 2,369,228 - -0.8 2,369,228 - +0.3 2,369,228 - -0.1 2,369,231 + -0.3 2,369,231 + +3.6 2,369,231 + -1.6 2,369,232 - +1.8 2,369,232 - -1.7 2,369,232 - +0.0 2,369,269 - +0.3 2,369,269 - -2.9 2,369,390 + +0.7 2,369,390 + +0.7 2,369,390 + +0.7 2,369,390 + -0.5 2,369,391 - +2.8 2,369,391 - -0.3 2,369,391 - -0.6 2,369,460 - +1.9 2,369,632 - +0.2 2,369,647 + -0.7 2,369,657 - -3.4 2,369,701 + +1.1 2,369,701 + -0.2 2,369,701 + +0.0 2,369,701 + -0.2 2,369,701 + +2.5 2,369,701 + +2.6 2,369,702 - +1.1 2,369,702 - +1.0 2,369,702 - +2.6 2,369,702 - -1.3 2,369,702 - -0.2 2,369,702 - +0.7 2,369,705 + +0.0 2,369,705 + +0.2 2,369,705 + +1.3 2,369,705 + +0.0 2,369,706 - +0.3 2,369,706 - +0.5 2,369,706 - +0.3 2,369,706 - +1.8
Or see this region's nucleotide sequence