Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1951 and Psyr_1952 are separated by 35 nucleotides Psyr_1952 and Psyr_1953 overlap by 4 nucleotides Psyr_1953 and Psyr_1954 overlap by 4 nucleotides Psyr_1954 and Psyr_1955 overlap by 4 nucleotides
Psyr_1951: Psyr_1951 - Periplasmic solute binding protein, at 2,235,967 to 2,236,899
_1951
Psyr_1952: Psyr_1952 - hypothetical protein, at 2,236,935 to 2,237,501
_1952
Psyr_1953: Psyr_1953 - conserved hypothetical protein, at 2,237,498 to 2,237,830
_1953
Psyr_1954: Psyr_1954 - conserved hypothetical protein, at 2,237,827 to 2,238,372
_1954
Psyr_1955: Psyr_1955 - Propeptide, PepSY amd peptidase M4:PepSY-associated TM helix, at 2,238,369 to 2,239,592
_1955
Position (kb)
2237
2238 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2236.633 kb on + strand, within Psyr_1951 at 2236.686 kb on - strand, within Psyr_1951 at 2236.757 kb on + strand, within Psyr_1951 at 2236.757 kb on + strand, within Psyr_1951 at 2236.757 kb on + strand, within Psyr_1951 at 2236.757 kb on + strand, within Psyr_1951 at 2236.758 kb on - strand, within Psyr_1951 at 2236.763 kb on + strand, within Psyr_1951 at 2236.763 kb on + strand, within Psyr_1951 at 2236.763 kb on + strand, within Psyr_1951 at 2236.763 kb on + strand, within Psyr_1951 at 2236.763 kb on + strand, within Psyr_1951 at 2236.764 kb on - strand, within Psyr_1951 at 2236.766 kb on + strand, within Psyr_1951 at 2236.780 kb on - strand, within Psyr_1951 at 2236.893 kb on + strand at 2236.893 kb on + strand at 2236.894 kb on - strand at 2236.894 kb on - strand at 2236.894 kb on - strand at 2237.138 kb on - strand, within Psyr_1952 at 2237.443 kb on + strand, within Psyr_1952 at 2237.444 kb on - strand, within Psyr_1952 at 2237.444 kb on - strand, within Psyr_1952 at 2237.444 kb on - strand, within Psyr_1952 at 2237.444 kb on - strand, within Psyr_1952 at 2237.480 kb on + strand at 2237.481 kb on - strand at 2237.481 kb on - strand at 2237.481 kb on - strand at 2237.566 kb on - strand, within Psyr_1953 at 2237.566 kb on - strand, within Psyr_1953 at 2237.566 kb on - strand, within Psyr_1953 at 2238.056 kb on + strand at 2238.057 kb on - strand, within Psyr_1954 at 2238.495 kb on - strand, within Psyr_1955 at 2238.538 kb on - strand, within Psyr_1955
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 2,236,633 + Psyr_1951 0.71 +0.3 2,236,686 - Psyr_1951 0.77 +0.0 2,236,757 + Psyr_1951 0.85 +0.4 2,236,757 + Psyr_1951 0.85 -0.3 2,236,757 + Psyr_1951 0.85 +1.5 2,236,757 + Psyr_1951 0.85 +0.8 2,236,758 - Psyr_1951 0.85 +1.0 2,236,763 + Psyr_1951 0.85 +1.6 2,236,763 + Psyr_1951 0.85 -1.6 2,236,763 + Psyr_1951 0.85 +0.0 2,236,763 + Psyr_1951 0.85 -0.8 2,236,763 + Psyr_1951 0.85 -0.2 2,236,764 - Psyr_1951 0.85 +0.2 2,236,766 + Psyr_1951 0.86 -0.5 2,236,780 - Psyr_1951 0.87 -3.6 2,236,893 + +0.1 2,236,893 + +0.0 2,236,894 - +0.9 2,236,894 - -0.6 2,236,894 - -0.6 2,237,138 - Psyr_1952 0.36 +0.9 2,237,443 + Psyr_1952 0.90 -0.3 2,237,444 - Psyr_1952 0.90 -1.1 2,237,444 - Psyr_1952 0.90 +1.5 2,237,444 - Psyr_1952 0.90 +1.0 2,237,444 - Psyr_1952 0.90 -0.3 2,237,480 + +0.8 2,237,481 - +0.4 2,237,481 - -0.4 2,237,481 - -0.7 2,237,566 - Psyr_1953 0.20 -2.2 2,237,566 - Psyr_1953 0.20 +0.0 2,237,566 - Psyr_1953 0.20 -1.7 2,238,056 + -1.3 2,238,057 - Psyr_1954 0.42 +1.4 2,238,495 - Psyr_1955 0.10 -1.3 2,238,538 - Psyr_1955 0.14 -0.5
Or see this region's nucleotide sequence