Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1870 and Psyr_1871 overlap by 4 nucleotides Psyr_1871 and Psyr_1872 are separated by 55 nucleotides Psyr_1872 and Psyr_1873 are separated by 609 nucleotides
Psyr_1870: Psyr_1870 - protein of unknown function DUF403, at 2,145,660 to 2,146,619
_1870
Psyr_1871: Psyr_1871 - Transglutaminase-like protein, at 2,146,616 to 2,147,446
_1871
Psyr_1872: Psyr_1872 - conserved hypothetical protein, at 2,147,502 to 2,147,873
_1872
Psyr_1873: Psyr_1873 - Glutamine amidotransferase class-I, at 2,148,483 to 2,149,211
_1873
Position (kb)
2147
2148 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2146.503 kb on + strand, within Psyr_1870 at 2146.504 kb on - strand, within Psyr_1870 at 2146.603 kb on - strand at 2146.646 kb on + strand at 2146.646 kb on + strand at 2146.647 kb on - strand at 2146.647 kb on - strand at 2146.652 kb on + strand at 2146.653 kb on - strand at 2146.653 kb on - strand at 2146.675 kb on + strand at 2146.683 kb on - strand at 2146.783 kb on + strand, within Psyr_1871 at 2146.783 kb on + strand, within Psyr_1871 at 2147.030 kb on + strand, within Psyr_1871 at 2147.030 kb on + strand, within Psyr_1871 at 2147.156 kb on + strand, within Psyr_1871 at 2147.157 kb on - strand, within Psyr_1871 at 2147.157 kb on - strand, within Psyr_1871 at 2147.226 kb on + strand, within Psyr_1871 at 2147.226 kb on + strand, within Psyr_1871 at 2147.309 kb on + strand, within Psyr_1871 at 2147.310 kb on - strand, within Psyr_1871 at 2147.444 kb on + strand at 2147.444 kb on + strand at 2147.444 kb on + strand at 2147.531 kb on - strand at 2147.554 kb on + strand, within Psyr_1872 at 2147.554 kb on + strand, within Psyr_1872 at 2147.555 kb on - strand, within Psyr_1872 at 2147.555 kb on - strand, within Psyr_1872 at 2147.555 kb on - strand, within Psyr_1872 at 2147.740 kb on + strand, within Psyr_1872 at 2147.754 kb on - strand, within Psyr_1872 at 2147.873 kb on + strand at 2147.873 kb on + strand at 2147.873 kb on + strand at 2147.873 kb on + strand at 2147.873 kb on + strand at 2147.874 kb on - strand at 2147.874 kb on - strand at 2147.874 kb on - strand at 2147.977 kb on + strand at 2147.988 kb on + strand at 2148.016 kb on + strand at 2148.016 kb on + strand at 2148.016 kb on + strand at 2148.017 kb on - strand at 2148.203 kb on + strand at 2148.203 kb on + strand at 2148.203 kb on + strand at 2148.203 kb on + strand at 2148.203 kb on + strand at 2148.204 kb on - strand at 2148.204 kb on - strand at 2148.204 kb on - strand at 2148.205 kb on + strand at 2148.206 kb on - strand at 2148.206 kb on - strand at 2148.211 kb on + strand at 2148.211 kb on + strand at 2148.211 kb on + strand at 2148.212 kb on - strand at 2148.212 kb on - strand at 2148.228 kb on - strand at 2148.228 kb on - strand at 2148.315 kb on + strand at 2148.373 kb on - strand at 2148.382 kb on - strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.490 kb on + strand at 2148.500 kb on + strand at 2148.552 kb on + strand at 2148.572 kb on - strand, within Psyr_1873 at 2148.572 kb on - strand, within Psyr_1873 at 2148.612 kb on + strand, within Psyr_1873 at 2148.613 kb on - strand, within Psyr_1873 at 2148.663 kb on + strand, within Psyr_1873 at 2148.664 kb on - strand, within Psyr_1873 at 2148.729 kb on + strand, within Psyr_1873 at 2148.729 kb on + strand, within Psyr_1873 at 2148.730 kb on - strand, within Psyr_1873 at 2148.730 kb on - strand, within Psyr_1873
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 2,146,503 + Psyr_1870 0.88 +0.2 2,146,504 - Psyr_1870 0.88 -0.6 2,146,603 - -0.4 2,146,646 + +0.1 2,146,646 + -0.4 2,146,647 - -0.3 2,146,647 - +1.0 2,146,652 + -1.5 2,146,653 - +3.0 2,146,653 - +0.9 2,146,675 + -0.1 2,146,683 - +0.9 2,146,783 + Psyr_1871 0.20 -2.3 2,146,783 + Psyr_1871 0.20 +1.8 2,147,030 + Psyr_1871 0.50 -2.0 2,147,030 + Psyr_1871 0.50 -0.6 2,147,156 + Psyr_1871 0.65 -0.3 2,147,157 - Psyr_1871 0.65 -0.5 2,147,157 - Psyr_1871 0.65 +0.6 2,147,226 + Psyr_1871 0.73 -0.4 2,147,226 + Psyr_1871 0.73 -0.1 2,147,309 + Psyr_1871 0.83 -0.3 2,147,310 - Psyr_1871 0.84 +0.5 2,147,444 + -2.6 2,147,444 + +0.5 2,147,444 + +0.1 2,147,531 - +3.3 2,147,554 + Psyr_1872 0.14 +4.2 2,147,554 + Psyr_1872 0.14 +1.4 2,147,555 - Psyr_1872 0.14 +0.7 2,147,555 - Psyr_1872 0.14 +1.0 2,147,555 - Psyr_1872 0.14 +0.1 2,147,740 + Psyr_1872 0.64 +0.5 2,147,754 - Psyr_1872 0.68 +0.4 2,147,873 + +0.2 2,147,873 + +0.9 2,147,873 + +0.3 2,147,873 + -0.5 2,147,873 + +0.8 2,147,874 - -0.3 2,147,874 - -0.4 2,147,874 - -0.8 2,147,977 + +0.2 2,147,988 + -0.4 2,148,016 + -1.0 2,148,016 + +0.3 2,148,016 + +0.3 2,148,017 - +0.2 2,148,203 + +1.8 2,148,203 + -1.5 2,148,203 + -2.5 2,148,203 + -1.8 2,148,203 + +0.7 2,148,204 - -0.3 2,148,204 - -0.5 2,148,204 - +0.8 2,148,205 + +0.0 2,148,206 - -0.4 2,148,206 - +1.3 2,148,211 + -1.0 2,148,211 + +0.5 2,148,211 + -0.8 2,148,212 - +1.0 2,148,212 - -0.2 2,148,228 - -1.3 2,148,228 - +0.5 2,148,315 + +1.0 2,148,373 - -3.4 2,148,382 - +0.5 2,148,482 + -0.6 2,148,482 + -0.3 2,148,482 + +1.7 2,148,482 + -0.8 2,148,482 + +0.7 2,148,482 + -0.1 2,148,482 + -2.8 2,148,482 + +0.0 2,148,482 + -1.0 2,148,482 + +0.0 2,148,482 + +0.0 2,148,483 - +1.0 2,148,483 - -0.3 2,148,483 - -0.3 2,148,483 - +2.3 2,148,483 - -0.6 2,148,483 - +0.1 2,148,490 + +3.0 2,148,500 + -0.6 2,148,552 + +0.1 2,148,572 - Psyr_1873 0.12 +1.3 2,148,572 - Psyr_1873 0.12 -0.6 2,148,612 + Psyr_1873 0.18 -0.8 2,148,613 - Psyr_1873 0.18 -4.0 2,148,663 + Psyr_1873 0.25 +0.2 2,148,664 - Psyr_1873 0.25 -1.0 2,148,729 + Psyr_1873 0.34 -3.3 2,148,729 + Psyr_1873 0.34 -1.4 2,148,730 - Psyr_1873 0.34 -0.4 2,148,730 - Psyr_1873 0.34 -0.8
Or see this region's nucleotide sequence