Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1727 and Psyr_1728 are separated by 84 nucleotides Psyr_1728 and Psyr_1729 are separated by 149 nucleotides Psyr_1729 and Psyr_1730 are separated by 72 nucleotides
Psyr_1727: Psyr_1727 - Protein of unknown function DUF1289, at 1,961,923 to 1,962,393
_1727
Psyr_1728: Psyr_1728 - tRNA--hydroxylase, at 1,962,478 to 1,963,089
_1728
Psyr_1729: Psyr_1729 - transcriptional regulator, MarR family, at 1,963,239 to 1,963,727
_1729
Psyr_1730: Psyr_1730 - Secretion protein HlyD, at 1,963,800 to 1,965,008
_1730
Position (kb)
1963
1964 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1962.352 kb on + strand at 1962.352 kb on + strand at 1962.353 kb on - strand at 1962.353 kb on - strand at 1962.353 kb on - strand at 1962.438 kb on + strand at 1962.438 kb on + strand at 1962.438 kb on + strand at 1962.438 kb on + strand at 1962.438 kb on + strand at 1962.438 kb on + strand at 1962.438 kb on + strand at 1962.439 kb on - strand at 1962.439 kb on - strand at 1962.439 kb on - strand at 1962.439 kb on - strand at 1962.441 kb on - strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.631 kb on + strand, within Psyr_1728 at 1962.632 kb on - strand, within Psyr_1728 at 1962.632 kb on - strand, within Psyr_1728 at 1962.761 kb on - strand, within Psyr_1728 at 1962.907 kb on + strand, within Psyr_1728 at 1962.950 kb on - strand, within Psyr_1728 at 1963.073 kb on + strand at 1963.074 kb on - strand at 1963.151 kb on - strand at 1963.153 kb on + strand at 1963.166 kb on - strand at 1963.188 kb on + strand at 1963.198 kb on + strand at 1963.199 kb on - strand at 1963.224 kb on + strand at 1963.224 kb on + strand at 1963.225 kb on - strand at 1963.225 kb on - strand at 1963.225 kb on - strand at 1963.299 kb on - strand, within Psyr_1729 at 1963.332 kb on + strand, within Psyr_1729 at 1963.333 kb on - strand, within Psyr_1729 at 1963.333 kb on - strand, within Psyr_1729 at 1963.333 kb on - strand, within Psyr_1729 at 1963.344 kb on + strand, within Psyr_1729 at 1963.344 kb on + strand, within Psyr_1729 at 1963.345 kb on - strand, within Psyr_1729 at 1963.351 kb on + strand, within Psyr_1729 at 1963.352 kb on - strand, within Psyr_1729 at 1963.352 kb on - strand, within Psyr_1729 at 1963.719 kb on - strand at 1963.786 kb on + strand at 1963.792 kb on + strand at 1963.932 kb on + strand, within Psyr_1730 at 1963.941 kb on + strand, within Psyr_1730 at 1963.941 kb on + strand, within Psyr_1730 at 1963.942 kb on - strand, within Psyr_1730 at 1963.974 kb on + strand at 1963.974 kb on + strand, within Psyr_1730 at 1963.974 kb on + strand, within Psyr_1730 at 1963.974 kb on + strand, within Psyr_1730 at 1963.974 kb on + strand, within Psyr_1730 at 1963.974 kb on + strand, within Psyr_1730 at 1963.975 kb on - strand, within Psyr_1730 at 1963.975 kb on - strand, within Psyr_1730 at 1963.975 kb on - strand, within Psyr_1730 at 1963.975 kb on - strand, within Psyr_1730 at 1963.975 kb on - strand, within Psyr_1730 at 1964.030 kb on + strand, within Psyr_1730 at 1964.031 kb on - strand, within Psyr_1730 at 1964.163 kb on + strand, within Psyr_1730 at 1964.165 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.235 kb on + strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.236 kb on - strand, within Psyr_1730 at 1964.436 kb on + strand, within Psyr_1730 at 1964.643 kb on + strand at 1964.661 kb on + strand, within Psyr_1730 at 1964.661 kb on + strand, within Psyr_1730 at 1964.661 kb on + strand, within Psyr_1730 at 1964.662 kb on - strand, within Psyr_1730
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 1,962,352 + +3.3 1,962,352 + -0.1 1,962,353 - -0.7 1,962,353 - +1.5 1,962,353 - -1.1 1,962,438 + +0.4 1,962,438 + -0.3 1,962,438 + -2.5 1,962,438 + -0.6 1,962,438 + +1.3 1,962,438 + -0.3 1,962,438 + +0.1 1,962,439 - -0.9 1,962,439 - -0.3 1,962,439 - -0.0 1,962,439 - +0.0 1,962,441 - +0.3 1,962,477 + -0.7 1,962,477 + -0.3 1,962,477 + +4.1 1,962,477 + -0.6 1,962,477 + -0.2 1,962,477 + -0.8 1,962,477 + -3.0 1,962,477 + -0.1 1,962,478 - +1.1 1,962,478 - +0.1 1,962,478 - +0.1 1,962,478 - -1.4 1,962,478 - +1.1 1,962,478 - +0.3 1,962,478 - +0.6 1,962,478 - +0.6 1,962,478 - +0.2 1,962,478 - +0.7 1,962,478 - +1.6 1,962,631 + Psyr_1728 0.25 +0.9 1,962,632 - Psyr_1728 0.25 -2.7 1,962,632 - Psyr_1728 0.25 -1.1 1,962,761 - Psyr_1728 0.46 +0.4 1,962,907 + Psyr_1728 0.70 -0.5 1,962,950 - Psyr_1728 0.77 +0.1 1,963,073 + +0.5 1,963,074 - -0.2 1,963,151 - +0.9 1,963,153 + +2.4 1,963,166 - +0.4 1,963,188 + +0.4 1,963,198 + +2.9 1,963,199 - +1.3 1,963,224 + -0.5 1,963,224 + +1.7 1,963,225 - -0.9 1,963,225 - +1.2 1,963,225 - -1.3 1,963,299 - Psyr_1729 0.12 -0.9 1,963,332 + Psyr_1729 0.19 -2.3 1,963,333 - Psyr_1729 0.19 -1.0 1,963,333 - Psyr_1729 0.19 +0.1 1,963,333 - Psyr_1729 0.19 -0.5 1,963,344 + Psyr_1729 0.21 +1.1 1,963,344 + Psyr_1729 0.21 -1.1 1,963,345 - Psyr_1729 0.22 -0.6 1,963,351 + Psyr_1729 0.23 -0.6 1,963,352 - Psyr_1729 0.23 -1.3 1,963,352 - Psyr_1729 0.23 +0.0 1,963,719 - -1.0 1,963,786 + +3.1 1,963,792 + +0.8 1,963,932 + Psyr_1730 0.11 -0.1 1,963,941 + Psyr_1730 0.12 -0.1 1,963,941 + Psyr_1730 0.12 -0.2 1,963,942 - Psyr_1730 0.12 -0.3 1,963,974 + +1.1 1,963,974 + Psyr_1730 0.14 -2.1 1,963,974 + Psyr_1730 0.14 +0.7 1,963,974 + Psyr_1730 0.14 -0.4 1,963,974 + Psyr_1730 0.14 +1.3 1,963,974 + Psyr_1730 0.14 -0.6 1,963,975 - Psyr_1730 0.14 -0.4 1,963,975 - Psyr_1730 0.14 -0.1 1,963,975 - Psyr_1730 0.14 +0.3 1,963,975 - Psyr_1730 0.14 -0.4 1,963,975 - Psyr_1730 0.14 +0.2 1,964,030 + Psyr_1730 0.19 +0.6 1,964,031 - Psyr_1730 0.19 -1.0 1,964,163 + Psyr_1730 0.30 -0.1 1,964,165 + Psyr_1730 0.30 -0.3 1,964,235 + Psyr_1730 0.36 -1.1 1,964,235 + Psyr_1730 0.36 +1.1 1,964,235 + Psyr_1730 0.36 -0.3 1,964,235 + Psyr_1730 0.36 +1.3 1,964,235 + Psyr_1730 0.36 -1.5 1,964,235 + Psyr_1730 0.36 -1.6 1,964,235 + Psyr_1730 0.36 +0.8 1,964,235 + Psyr_1730 0.36 -1.1 1,964,235 + Psyr_1730 0.36 -0.3 1,964,235 + Psyr_1730 0.36 +1.3 1,964,235 + Psyr_1730 0.36 -1.3 1,964,236 - Psyr_1730 0.36 -1.0 1,964,236 - Psyr_1730 0.36 +2.1 1,964,236 - Psyr_1730 0.36 -0.1 1,964,236 - Psyr_1730 0.36 +1.2 1,964,236 - Psyr_1730 0.36 -0.7 1,964,236 - Psyr_1730 0.36 -0.7 1,964,236 - Psyr_1730 0.36 +1.5 1,964,236 - Psyr_1730 0.36 -2.1 1,964,236 - Psyr_1730 0.36 -1.7 1,964,236 - Psyr_1730 0.36 +0.5 1,964,236 - Psyr_1730 0.36 -0.1 1,964,236 - Psyr_1730 0.36 +2.9 1,964,236 - Psyr_1730 0.36 -2.5 1,964,236 - Psyr_1730 0.36 -2.6 1,964,236 - Psyr_1730 0.36 +1.3 1,964,236 - Psyr_1730 0.36 +0.6 1,964,236 - Psyr_1730 0.36 -0.1 1,964,436 + Psyr_1730 0.53 +0.7 1,964,643 + +1.8 1,964,661 + Psyr_1730 0.71 +0.1 1,964,661 + Psyr_1730 0.71 +0.1 1,964,661 + Psyr_1730 0.71 -0.5 1,964,662 - Psyr_1730 0.71 +0.5
Or see this region's nucleotide sequence