Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1612 and Psyr_1613 are separated by 231 nucleotides Psyr_1613 and Psyr_1614 are separated by 120 nucleotides
Psyr_1612: Psyr_1612 - septum site-determining protein MinD, at 1,814,844 to 1,815,656
_1612
Psyr_1613: Psyr_1613 - septum site-determining protein MinC, at 1,815,888 to 1,816,628
_1613
Psyr_1614: Psyr_1614 - lipid A biosynthesis acyltransferase, at 1,816,749 to 1,817,681
_1614
Position (kb)
1815
1816
1817 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1815.313 kb on + strand, within Psyr_1612 at 1815.314 kb on - strand, within Psyr_1612 at 1815.636 kb on - strand at 1815.770 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.982 kb on + strand, within Psyr_1613 at 1815.982 kb on + strand, within Psyr_1613 at 1815.982 kb on + strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1816.088 kb on - strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.473 kb on - strand, within Psyr_1613 at 1816.594 kb on - strand at 1816.594 kb on - strand at 1816.635 kb on + strand at 1816.635 kb on + strand at 1816.636 kb on - strand at 1816.637 kb on + strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.673 kb on + strand at 1816.714 kb on + strand at 1816.714 kb on + strand at 1816.715 kb on - strand at 1816.715 kb on - strand at 1816.715 kb on - strand at 1816.715 kb on - strand at 1816.891 kb on - strand, within Psyr_1614 at 1816.891 kb on - strand, within Psyr_1614 at 1817.154 kb on + strand, within Psyr_1614 at 1817.202 kb on + strand, within Psyr_1614 at 1817.202 kb on + strand, within Psyr_1614 at 1817.203 kb on - strand, within Psyr_1614 at 1817.315 kb on - strand, within Psyr_1614 at 1817.341 kb on - strand, within Psyr_1614 at 1817.611 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 1,815,313 + Psyr_1612 0.58 -0.3 1,815,314 - Psyr_1612 0.58 -3.5 1,815,636 - -0.6 1,815,770 - +0.2 1,815,791 - +2.9 1,815,791 - -2.6 1,815,791 - -1.1 1,815,791 - +1.3 1,815,791 - -0.5 1,815,982 + Psyr_1613 0.13 -2.3 1,815,982 + Psyr_1613 0.13 -1.4 1,815,982 + Psyr_1613 0.13 -2.4 1,815,983 - Psyr_1613 0.13 -2.0 1,815,983 - Psyr_1613 0.13 -1.3 1,816,088 - Psyr_1613 0.27 +1.2 1,816,168 + Psyr_1613 0.38 -4.0 1,816,168 + Psyr_1613 0.38 -3.0 1,816,168 + Psyr_1613 0.38 -3.4 1,816,168 + Psyr_1613 0.38 -0.7 1,816,169 - Psyr_1613 0.38 -3.0 1,816,169 - Psyr_1613 0.38 -2.0 1,816,169 - Psyr_1613 0.38 -1.7 1,816,473 - Psyr_1613 0.79 +0.2 1,816,594 - -3.8 1,816,594 - -3.1 1,816,635 + -3.5 1,816,635 + -2.7 1,816,636 - +0.9 1,816,637 + -2.7 1,816,638 - -0.7 1,816,638 - +1.5 1,816,638 - -0.2 1,816,638 - -1.0 1,816,638 - +0.7 1,816,638 - -0.6 1,816,638 - -1.0 1,816,638 - +2.0 1,816,638 - -0.7 1,816,672 - -0.4 1,816,672 - +0.8 1,816,672 - +1.8 1,816,672 - -0.9 1,816,672 - +0.4 1,816,672 - +0.8 1,816,673 + -2.2 1,816,714 + -1.2 1,816,714 + -1.7 1,816,715 - -0.6 1,816,715 - -0.8 1,816,715 - -0.3 1,816,715 - -1.2 1,816,891 - Psyr_1614 0.15 +1.2 1,816,891 - Psyr_1614 0.15 +1.2 1,817,154 + Psyr_1614 0.43 +1.2 1,817,202 + Psyr_1614 0.49 +1.2 1,817,202 + Psyr_1614 0.49 +1.2 1,817,203 - Psyr_1614 0.49 +0.2 1,817,315 - Psyr_1614 0.61 +1.2 1,817,341 - Psyr_1614 0.63 -2.1 1,817,611 - -0.8
Or see this region's nucleotide sequence