Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1574 and Psyr_1575 are separated by 132 nucleotides Psyr_1575 and Psyr_1576 are separated by 76 nucleotides Psyr_1576 and Psyr_1577 are separated by 46 nucleotides Psyr_1577 and Psyr_1578 are separated by 37 nucleotides
Psyr_1574: Psyr_1574 - oligopeptidase B, Serine peptidase, MEROPS family S09A, at 1,768,583 to 1,770,643
_1574
Psyr_1575: Psyr_1575 - conserved hypothetical protein, at 1,770,776 to 1,771,252
_1575
Psyr_1576: Psyr_1576 - Glutamine amidotransferase, class-II, at 1,771,329 to 1,772,129
_1576
Psyr_1577: Psyr_1577 - conserved hypothetical protein, at 1,772,176 to 1,772,700
_1577
Psyr_1578: Psyr_1578 - conserved hypothetical protein, at 1,772,738 to 1,773,454
_1578
Position (kb)
1771
1772
1773 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1770.369 kb on + strand, within Psyr_1574 at 1770.370 kb on - strand, within Psyr_1574 at 1770.370 kb on - strand, within Psyr_1574 at 1770.396 kb on + strand, within Psyr_1574 at 1770.397 kb on - strand, within Psyr_1574 at 1770.397 kb on - strand, within Psyr_1574 at 1770.399 kb on + strand, within Psyr_1574 at 1770.405 kb on + strand, within Psyr_1574 at 1770.406 kb on - strand, within Psyr_1574 at 1770.406 kb on - strand, within Psyr_1574 at 1770.580 kb on - strand at 1770.659 kb on - strand at 1770.678 kb on + strand at 1770.743 kb on - strand at 1770.763 kb on + strand at 1770.791 kb on + strand at 1770.920 kb on - strand, within Psyr_1575 at 1770.920 kb on - strand, within Psyr_1575 at 1770.920 kb on - strand, within Psyr_1575 at 1771.009 kb on + strand, within Psyr_1575 at 1771.010 kb on - strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.187 kb on + strand, within Psyr_1575 at 1771.251 kb on - strand at 1771.339 kb on + strand at 1771.449 kb on + strand, within Psyr_1576 at 1771.450 kb on - strand, within Psyr_1576 at 1771.450 kb on - strand, within Psyr_1576 at 1771.559 kb on + strand, within Psyr_1576 at 1771.632 kb on - strand, within Psyr_1576 at 1771.827 kb on - strand, within Psyr_1576 at 1771.827 kb on - strand, within Psyr_1576 at 1771.883 kb on + strand, within Psyr_1576 at 1772.130 kb on + strand at 1772.131 kb on - strand at 1772.131 kb on - strand at 1772.149 kb on - strand at 1772.188 kb on + strand at 1772.257 kb on + strand, within Psyr_1577 at 1772.258 kb on - strand, within Psyr_1577 at 1772.312 kb on - strand, within Psyr_1577 at 1772.314 kb on - strand, within Psyr_1577 at 1772.368 kb on + strand, within Psyr_1577 at 1772.369 kb on - strand, within Psyr_1577 at 1772.547 kb on + strand, within Psyr_1577 at 1772.547 kb on + strand, within Psyr_1577 at 1772.547 kb on + strand, within Psyr_1577 at 1772.547 kb on + strand, within Psyr_1577 at 1772.547 kb on + strand, within Psyr_1577 at 1772.547 kb on + strand, within Psyr_1577 at 1772.547 kb on + strand, within Psyr_1577 at 1772.547 kb on + strand, within Psyr_1577 at 1772.547 kb on + strand, within Psyr_1577 at 1772.548 kb on - strand, within Psyr_1577 at 1772.548 kb on - strand, within Psyr_1577 at 1772.548 kb on - strand, within Psyr_1577 at 1772.548 kb on - strand, within Psyr_1577 at 1772.739 kb on + strand at 1772.739 kb on + strand at 1772.739 kb on + strand at 1772.739 kb on + strand at 1772.739 kb on + strand at 1772.739 kb on + strand at 1772.739 kb on + strand at 1772.739 kb on + strand at 1772.739 kb on + strand at 1772.740 kb on - strand at 1772.740 kb on - strand at 1772.740 kb on - strand at 1772.740 kb on - strand at 1772.740 kb on - strand at 1772.740 kb on - strand at 1772.740 kb on - strand at 1772.740 kb on - strand at 1772.740 kb on - strand at 1772.740 kb on - strand at 1772.741 kb on + strand at 1772.741 kb on + strand at 1772.741 kb on + strand at 1772.741 kb on + strand at 1772.741 kb on + strand at 1772.741 kb on + strand at 1772.741 kb on + strand at 1772.742 kb on - strand at 1772.742 kb on - strand at 1772.742 kb on - strand at 1772.742 kb on - strand at 1772.742 kb on - strand at 1772.761 kb on + strand at 1772.761 kb on + strand at 1772.761 kb on + strand at 1772.762 kb on - strand at 1772.762 kb on - strand at 1772.762 kb on - strand at 1772.765 kb on + strand at 1772.765 kb on + strand at 1772.765 kb on + strand at 1772.765 kb on + strand at 1772.765 kb on + strand at 1772.766 kb on - strand at 1772.766 kb on - strand at 1772.766 kb on - strand at 1772.766 kb on - strand at 1772.769 kb on + strand at 1772.888 kb on + strand, within Psyr_1578 at 1772.888 kb on + strand, within Psyr_1578 at 1772.888 kb on + strand, within Psyr_1578 at 1772.889 kb on - strand, within Psyr_1578 at 1772.889 kb on - strand, within Psyr_1578 at 1772.949 kb on + strand, within Psyr_1578 at 1772.949 kb on + strand, within Psyr_1578 at 1772.949 kb on + strand, within Psyr_1578 at 1772.950 kb on - strand, within Psyr_1578 at 1772.975 kb on + strand, within Psyr_1578 at 1772.976 kb on - strand, within Psyr_1578 at 1772.976 kb on - strand, within Psyr_1578 at 1773.062 kb on + strand, within Psyr_1578 at 1773.063 kb on - strand, within Psyr_1578 at 1773.063 kb on - strand, within Psyr_1578 at 1773.063 kb on - strand, within Psyr_1578 at 1773.063 kb on - strand, within Psyr_1578 at 1773.066 kb on + strand, within Psyr_1578
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 1,770,369 + Psyr_1574 0.87 +0.3 1,770,370 - Psyr_1574 0.87 +0.8 1,770,370 - Psyr_1574 0.87 +0.2 1,770,396 + Psyr_1574 0.88 +1.8 1,770,397 - Psyr_1574 0.88 -2.7 1,770,397 - Psyr_1574 0.88 -2.5 1,770,399 + Psyr_1574 0.88 +0.6 1,770,405 + Psyr_1574 0.88 -0.3 1,770,406 - Psyr_1574 0.88 -0.7 1,770,406 - Psyr_1574 0.88 +0.5 1,770,580 - -2.5 1,770,659 - +0.2 1,770,678 + +0.9 1,770,743 - +0.8 1,770,763 + -0.8 1,770,791 + -0.6 1,770,920 - Psyr_1575 0.30 -0.9 1,770,920 - Psyr_1575 0.30 -1.0 1,770,920 - Psyr_1575 0.30 -0.4 1,771,009 + Psyr_1575 0.49 -0.3 1,771,010 - Psyr_1575 0.49 +0.3 1,771,040 + Psyr_1575 0.55 +0.2 1,771,040 + Psyr_1575 0.55 -0.3 1,771,040 + Psyr_1575 0.55 -3.2 1,771,040 + Psyr_1575 0.55 +2.8 1,771,040 + Psyr_1575 0.55 -0.4 1,771,040 + Psyr_1575 0.55 -0.6 1,771,040 + Psyr_1575 0.55 -0.3 1,771,040 + Psyr_1575 0.55 +1.0 1,771,040 + Psyr_1575 0.55 +1.2 1,771,040 + Psyr_1575 0.55 -0.4 1,771,040 + Psyr_1575 0.55 +1.2 1,771,040 + Psyr_1575 0.55 -0.6 1,771,040 + Psyr_1575 0.55 -0.8 1,771,040 + Psyr_1575 0.55 +1.2 1,771,040 + Psyr_1575 0.55 +0.1 1,771,040 + Psyr_1575 0.55 -0.5 1,771,040 + Psyr_1575 0.55 +0.3 1,771,040 + Psyr_1575 0.55 +0.6 1,771,040 + Psyr_1575 0.55 -0.0 1,771,040 + Psyr_1575 0.55 -0.4 1,771,040 + Psyr_1575 0.55 -0.8 1,771,040 + Psyr_1575 0.55 +1.5 1,771,040 + Psyr_1575 0.55 +0.3 1,771,040 + Psyr_1575 0.55 -1.2 1,771,040 + Psyr_1575 0.55 +0.0 1,771,040 + Psyr_1575 0.55 +2.2 1,771,041 - Psyr_1575 0.56 +1.3 1,771,041 - Psyr_1575 0.56 -0.9 1,771,041 - Psyr_1575 0.56 +0.1 1,771,041 - Psyr_1575 0.56 -1.2 1,771,041 - Psyr_1575 0.56 +0.5 1,771,041 - Psyr_1575 0.56 -3.2 1,771,041 - Psyr_1575 0.56 -0.2 1,771,041 - Psyr_1575 0.56 +0.3 1,771,041 - Psyr_1575 0.56 -1.6 1,771,041 - Psyr_1575 0.56 +1.8 1,771,041 - Psyr_1575 0.56 +1.4 1,771,041 - Psyr_1575 0.56 -0.5 1,771,041 - Psyr_1575 0.56 +2.4 1,771,041 - Psyr_1575 0.56 +0.2 1,771,041 - Psyr_1575 0.56 -0.4 1,771,041 - Psyr_1575 0.56 +0.4 1,771,041 - Psyr_1575 0.56 -1.6 1,771,041 - Psyr_1575 0.56 -0.8 1,771,041 - Psyr_1575 0.56 -1.1 1,771,041 - Psyr_1575 0.56 -3.6 1,771,041 - Psyr_1575 0.56 -1.4 1,771,041 - Psyr_1575 0.56 +1.9 1,771,041 - Psyr_1575 0.56 -2.9 1,771,045 + Psyr_1575 0.56 -0.8 1,771,045 + Psyr_1575 0.56 -1.2 1,771,045 + Psyr_1575 0.56 -1.4 1,771,045 + Psyr_1575 0.56 -2.6 1,771,045 + Psyr_1575 0.56 +0.2 1,771,045 + Psyr_1575 0.56 -0.6 1,771,045 + Psyr_1575 0.56 -1.8 1,771,045 + Psyr_1575 0.56 -0.4 1,771,045 + Psyr_1575 0.56 +0.4 1,771,046 - Psyr_1575 0.57 +3.5 1,771,046 - Psyr_1575 0.57 +0.9 1,771,046 - Psyr_1575 0.57 +0.4 1,771,046 - Psyr_1575 0.57 -0.9 1,771,046 - Psyr_1575 0.57 +0.4 1,771,046 - Psyr_1575 0.57 -0.6 1,771,046 - Psyr_1575 0.57 +1.3 1,771,187 + Psyr_1575 0.86 +0.4 1,771,251 - +0.5 1,771,339 + +1.9 1,771,449 + Psyr_1576 0.15 +1.2 1,771,450 - Psyr_1576 0.15 -0.8 1,771,450 - Psyr_1576 0.15 +0.1 1,771,559 + Psyr_1576 0.29 +1.2 1,771,632 - Psyr_1576 0.38 +0.6 1,771,827 - Psyr_1576 0.62 -1.5 1,771,827 - Psyr_1576 0.62 -0.5 1,771,883 + Psyr_1576 0.69 -1.6 1,772,130 + +0.6 1,772,131 - +1.2 1,772,131 - +0.0 1,772,149 - -2.2 1,772,188 + +0.2 1,772,257 + Psyr_1577 0.15 -2.2 1,772,258 - Psyr_1577 0.16 +2.8 1,772,312 - Psyr_1577 0.26 +0.5 1,772,314 - Psyr_1577 0.26 +0.4 1,772,368 + Psyr_1577 0.37 +0.0 1,772,369 - Psyr_1577 0.37 +0.2 1,772,547 + Psyr_1577 0.71 -0.3 1,772,547 + Psyr_1577 0.71 +1.4 1,772,547 + Psyr_1577 0.71 +3.2 1,772,547 + Psyr_1577 0.71 +0.8 1,772,547 + Psyr_1577 0.71 +1.0 1,772,547 + Psyr_1577 0.71 -0.7 1,772,547 + Psyr_1577 0.71 -0.8 1,772,547 + Psyr_1577 0.71 -2.4 1,772,547 + Psyr_1577 0.71 +2.0 1,772,548 - Psyr_1577 0.71 +0.2 1,772,548 - Psyr_1577 0.71 -0.3 1,772,548 - Psyr_1577 0.71 -0.1 1,772,548 - Psyr_1577 0.71 -1.3 1,772,739 + -2.6 1,772,739 + +1.2 1,772,739 + +0.8 1,772,739 + -1.6 1,772,739 + -2.4 1,772,739 + -0.5 1,772,739 + -0.9 1,772,739 + -1.0 1,772,739 + -1.2 1,772,740 - +0.2 1,772,740 - +2.2 1,772,740 - -0.4 1,772,740 - -0.0 1,772,740 - +1.0 1,772,740 - -0.5 1,772,740 - -2.0 1,772,740 - +0.2 1,772,740 - -0.6 1,772,740 - -2.0 1,772,741 + -0.3 1,772,741 + +3.8 1,772,741 + +0.9 1,772,741 + +1.6 1,772,741 + -2.2 1,772,741 + -0.2 1,772,741 + +1.2 1,772,742 - +0.2 1,772,742 - +1.3 1,772,742 - +3.0 1,772,742 - -2.2 1,772,742 - +0.3 1,772,761 + -0.2 1,772,761 + +1.9 1,772,761 + -0.9 1,772,762 - -0.1 1,772,762 - -1.2 1,772,762 - +0.5 1,772,765 + -1.8 1,772,765 + -0.2 1,772,765 + -0.2 1,772,765 + +0.8 1,772,765 + -0.3 1,772,766 - +0.3 1,772,766 - +0.2 1,772,766 - -1.8 1,772,766 - +0.0 1,772,769 + +0.6 1,772,888 + Psyr_1578 0.21 -1.3 1,772,888 + Psyr_1578 0.21 +0.9 1,772,888 + Psyr_1578 0.21 -0.5 1,772,889 - Psyr_1578 0.21 -0.4 1,772,889 - Psyr_1578 0.21 -1.7 1,772,949 + Psyr_1578 0.29 -0.0 1,772,949 + Psyr_1578 0.29 -0.6 1,772,949 + Psyr_1578 0.29 +1.2 1,772,950 - Psyr_1578 0.30 -0.1 1,772,975 + Psyr_1578 0.33 -0.8 1,772,976 - Psyr_1578 0.33 -0.8 1,772,976 - Psyr_1578 0.33 -0.8 1,773,062 + Psyr_1578 0.45 -1.3 1,773,063 - Psyr_1578 0.45 +2.5 1,773,063 - Psyr_1578 0.45 -0.9 1,773,063 - Psyr_1578 0.45 -0.9 1,773,063 - Psyr_1578 0.45 +0.0 1,773,066 + Psyr_1578 0.46 +0.6
Or see this region's nucleotide sequence