Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1378 and Psyr_1379 are separated by 5 nucleotides Psyr_1379 and Psyr_1380 are separated by 13 nucleotides
Psyr_1378: Psyr_1378 - RecA protein, at 1,562,480 to 1,563,544
_1378
Psyr_1379: Psyr_1379 - Regulatory protein RecX, at 1,563,550 to 1,564,017
_1379
Psyr_1380: Psyr_1380 - Conserved hypothetical protein 730, at 1,564,031 to 1,565,143
_1380
Position (kb)
1563
1564
1565 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1563.128 kb on + strand, within Psyr_1378 at 1563.129 kb on - strand, within Psyr_1378 at 1563.281 kb on + strand, within Psyr_1378 at 1563.281 kb on + strand, within Psyr_1378 at 1563.282 kb on - strand, within Psyr_1378 at 1563.282 kb on - strand, within Psyr_1378 at 1563.353 kb on + strand, within Psyr_1378 at 1563.517 kb on + strand at 1563.517 kb on + strand at 1563.517 kb on + strand at 1563.518 kb on - strand at 1563.518 kb on - strand at 1563.518 kb on - strand at 1563.518 kb on - strand at 1563.518 kb on - strand at 1563.518 kb on - strand at 1563.643 kb on - strand, within Psyr_1379 at 1563.784 kb on - strand, within Psyr_1379 at 1563.874 kb on + strand, within Psyr_1379 at 1563.956 kb on - strand, within Psyr_1379 at 1564.018 kb on + strand at 1564.018 kb on + strand at 1564.018 kb on + strand at 1564.018 kb on + strand at 1564.018 kb on + strand at 1564.018 kb on + strand at 1564.018 kb on + strand at 1564.018 kb on + strand at 1564.019 kb on - strand at 1564.019 kb on - strand at 1564.019 kb on - strand at 1564.019 kb on - strand at 1564.019 kb on - strand at 1564.019 kb on - strand at 1564.021 kb on - strand at 1564.032 kb on + strand at 1564.032 kb on + strand at 1564.104 kb on + strand at 1564.104 kb on + strand at 1564.104 kb on + strand at 1564.104 kb on + strand at 1564.104 kb on + strand at 1564.105 kb on - strand at 1564.105 kb on - strand at 1564.105 kb on - strand at 1564.105 kb on - strand at 1564.105 kb on - strand at 1564.105 kb on - strand at 1564.105 kb on - strand at 1564.105 kb on - strand at 1564.207 kb on - strand, within Psyr_1380 at 1564.298 kb on - strand, within Psyr_1380 at 1564.347 kb on + strand, within Psyr_1380 at 1564.348 kb on - strand at 1564.425 kb on + strand, within Psyr_1380 at 1564.425 kb on + strand, within Psyr_1380 at 1564.425 kb on + strand, within Psyr_1380 at 1564.426 kb on - strand, within Psyr_1380 at 1564.426 kb on - strand, within Psyr_1380 at 1564.426 kb on - strand, within Psyr_1380 at 1564.426 kb on - strand, within Psyr_1380 at 1564.502 kb on + strand, within Psyr_1380 at 1564.503 kb on - strand, within Psyr_1380 at 1564.535 kb on + strand, within Psyr_1380 at 1564.535 kb on + strand, within Psyr_1380 at 1564.536 kb on - strand, within Psyr_1380 at 1564.536 kb on - strand, within Psyr_1380 at 1564.674 kb on - strand, within Psyr_1380 at 1564.861 kb on + strand, within Psyr_1380 at 1564.910 kb on + strand, within Psyr_1380 at 1564.910 kb on + strand, within Psyr_1380 at 1564.910 kb on + strand, within Psyr_1380 at 1564.910 kb on + strand, within Psyr_1380 at 1564.911 kb on - strand, within Psyr_1380 at 1564.956 kb on - strand, within Psyr_1380
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 1,563,128 + Psyr_1378 0.61 +0.2 1,563,129 - Psyr_1378 0.61 -1.4 1,563,281 + Psyr_1378 0.75 -1.0 1,563,281 + Psyr_1378 0.75 -2.3 1,563,282 - Psyr_1378 0.75 -2.0 1,563,282 - Psyr_1378 0.75 +0.1 1,563,353 + Psyr_1378 0.82 -0.6 1,563,517 + -1.1 1,563,517 + -0.0 1,563,517 + -1.7 1,563,518 - -1.3 1,563,518 - +1.5 1,563,518 - +1.2 1,563,518 - -2.0 1,563,518 - -0.7 1,563,518 - -1.6 1,563,643 - Psyr_1379 0.20 -1.3 1,563,784 - Psyr_1379 0.50 -0.1 1,563,874 + Psyr_1379 0.69 -1.0 1,563,956 - Psyr_1379 0.87 -0.1 1,564,018 + +0.5 1,564,018 + +1.6 1,564,018 + +0.1 1,564,018 + -0.7 1,564,018 + -2.3 1,564,018 + +0.5 1,564,018 + -0.9 1,564,018 + -1.3 1,564,019 - -0.0 1,564,019 - +0.9 1,564,019 - -0.3 1,564,019 - +1.4 1,564,019 - -0.2 1,564,019 - -0.8 1,564,021 - -0.1 1,564,032 + +0.3 1,564,032 + +0.6 1,564,104 + -1.4 1,564,104 + -0.1 1,564,104 + -0.2 1,564,104 + -0.7 1,564,104 + +0.8 1,564,105 - -0.3 1,564,105 - -0.8 1,564,105 - +0.0 1,564,105 - -0.4 1,564,105 - +0.2 1,564,105 - -0.3 1,564,105 - +0.1 1,564,105 - +1.5 1,564,207 - Psyr_1380 0.16 -0.8 1,564,298 - Psyr_1380 0.24 +0.3 1,564,347 + Psyr_1380 0.28 +0.4 1,564,348 - +0.2 1,564,425 + Psyr_1380 0.35 -1.1 1,564,425 + Psyr_1380 0.35 -1.8 1,564,425 + Psyr_1380 0.35 -0.4 1,564,426 - Psyr_1380 0.35 +1.1 1,564,426 - Psyr_1380 0.35 -3.0 1,564,426 - Psyr_1380 0.35 +0.9 1,564,426 - Psyr_1380 0.35 -0.1 1,564,502 + Psyr_1380 0.42 +1.8 1,564,503 - Psyr_1380 0.42 -3.6 1,564,535 + Psyr_1380 0.45 -1.3 1,564,535 + Psyr_1380 0.45 +2.0 1,564,536 - Psyr_1380 0.45 +0.2 1,564,536 - Psyr_1380 0.45 -1.6 1,564,674 - Psyr_1380 0.58 +0.8 1,564,861 + Psyr_1380 0.75 -0.2 1,564,910 + Psyr_1380 0.79 +1.4 1,564,910 + Psyr_1380 0.79 -1.2 1,564,910 + Psyr_1380 0.79 +0.0 1,564,910 + Psyr_1380 0.79 +0.2 1,564,911 - Psyr_1380 0.79 -2.9 1,564,956 - Psyr_1380 0.83 -1.0
Or see this region's nucleotide sequence