Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1235 and Psyr_1236 are separated by 159 nucleotides Psyr_1236 and Psyr_1237 are separated by 44 nucleotides Psyr_1237 and Psyr_1238 are separated by 81 nucleotides Psyr_1238 and Psyr_1239 are separated by 71 nucleotides Psyr_1239 and Psyr_1240 overlap by 23 nucleotides
Psyr_1235: Psyr_1235 - serine O-acetyltransferase, at 1,393,300 to 1,394,076
_1235
Psyr_1236: Psyr_1236 - transcriptional regulator, BadM/Rrf2 family, at 1,394,236 to 1,394,727
_1236
Psyr_1237: Psyr_1237 - cysteine desulfurase IscS, at 1,394,772 to 1,395,986
_1237
Psyr_1238: Psyr_1238 - FeS cluster assembly scaffold IscU, at 1,396,068 to 1,396,454
_1238
Psyr_1239: Psyr_1239 - HesB/YadR/YfhF:Iron-sulfur cluster assembly protein IscA, at 1,396,526 to 1,396,849
_1239
Psyr_1240: Psyr_1240 - Co-chaperone Hsc20, at 1,396,827 to 1,397,381
_1240
Position (kb)
1394
1395
1396 Strain fitness (log2 ratio)
-2
-1
0
1 at 1393.887 kb on + strand, within Psyr_1235 at 1394.105 kb on + strand at 1394.105 kb on + strand at 1394.106 kb on - strand at 1394.121 kb on + strand at 1394.122 kb on - strand at 1394.200 kb on + strand at 1394.200 kb on + strand at 1394.200 kb on + strand at 1394.247 kb on + strand at 1394.247 kb on + strand at 1394.343 kb on + strand, within Psyr_1236 at 1394.343 kb on + strand, within Psyr_1236 at 1394.356 kb on + strand, within Psyr_1236 at 1394.639 kb on + strand, within Psyr_1236 at 1394.700 kb on + strand at 1395.984 kb on + strand at 1395.989 kb on + strand at 1396.004 kb on + strand at 1396.004 kb on + strand at 1396.856 kb on + strand at 1396.856 kb on + strand at 1396.856 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 1,393,887 + Psyr_1235 0.76 +0.5 1,394,105 + -1.2 1,394,105 + +0.1 1,394,106 - -0.5 1,394,121 + +0.3 1,394,122 - +1.0 1,394,200 + +1.3 1,394,200 + -0.8 1,394,200 + +0.4 1,394,247 + +0.0 1,394,247 + +0.3 1,394,343 + Psyr_1236 0.22 -0.8 1,394,343 + Psyr_1236 0.22 -0.4 1,394,356 + Psyr_1236 0.24 -2.4 1,394,639 + Psyr_1236 0.82 -0.8 1,394,700 + +0.3 1,395,984 + -2.5 1,395,989 + +1.4 1,396,004 + -0.2 1,396,004 + +0.2 1,396,856 + -0.4 1,396,856 + -0.1 1,396,856 + +1.2
Or see this region's nucleotide sequence