Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0475 and Psyr_0476 are separated by 25 nucleotides Psyr_0476 and Psyr_0477 are separated by 21 nucleotides Psyr_0477 and Psyr_0478 are separated by 55 nucleotides
Psyr_0475: Psyr_0475 - Protein of unknown function YGGT, at 514,265 to 514,855
_0475
Psyr_0476: Psyr_0476 - pyrroline-5-carboxylate reductase, at 514,881 to 515,699
_0476
Psyr_0477: Psyr_0477 - Protein of unknown function UPF0001, at 515,721 to 516,407
_0477
Psyr_0478: Psyr_0478 - pilus retraction ATPase PilT, at 516,463 to 517,497
_0478
Position (kb)
515
516
517 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.788 kb on - strand, within Psyr_0475 at 514.800 kb on + strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 515.167 kb on + strand, within Psyr_0476 at 515.496 kb on + strand, within Psyr_0476 at 515.734 kb on + strand at 515.996 kb on + strand, within Psyr_0477 at 516.117 kb on + strand, within Psyr_0477 at 516.117 kb on + strand, within Psyr_0477 at 516.118 kb on - strand, within Psyr_0477 at 516.236 kb on - strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.302 kb on + strand, within Psyr_0477 at 516.444 kb on + strand at 516.444 kb on + strand at 516.444 kb on + strand at 516.444 kb on + strand at 516.444 kb on + strand at 516.444 kb on + strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.462 kb on + strand at 516.463 kb on - strand at 516.463 kb on - strand at 516.469 kb on - strand at 516.570 kb on + strand, within Psyr_0478 at 516.616 kb on + strand, within Psyr_0478 at 516.616 kb on + strand, within Psyr_0478 at 516.616 kb on + strand, within Psyr_0478 at 516.616 kb on + strand, within Psyr_0478 at 516.617 kb on - strand, within Psyr_0478 at 516.617 kb on - strand, within Psyr_0478 at 516.617 kb on - strand, within Psyr_0478 at 516.617 kb on - strand, within Psyr_0478 at 516.817 kb on - strand, within Psyr_0478 at 516.898 kb on + strand, within Psyr_0478 at 516.899 kb on - strand, within Psyr_0478 at 516.899 kb on - strand, within Psyr_0478 at 516.906 kb on + strand, within Psyr_0478 at 516.912 kb on + strand, within Psyr_0478 at 516.912 kb on + strand, within Psyr_0478 at 516.912 kb on + strand, within Psyr_0478 at 516.912 kb on + strand, within Psyr_0478 at 516.913 kb on - strand, within Psyr_0478 at 516.924 kb on + strand, within Psyr_0478 at 516.924 kb on + strand, within Psyr_0478 at 516.924 kb on + strand, within Psyr_0478 at 516.925 kb on - strand, within Psyr_0478 at 516.925 kb on - strand, within Psyr_0478 at 516.925 kb on - strand, within Psyr_0478 at 516.925 kb on - strand, within Psyr_0478 at 517.133 kb on + strand, within Psyr_0478 at 517.366 kb on + strand, within Psyr_0478 at 517.366 kb on + strand, within Psyr_0478 at 517.366 kb on + strand, within Psyr_0478 at 517.366 kb on + strand, within Psyr_0478 at 517.366 kb on + strand, within Psyr_0478 at 517.367 kb on - strand, within Psyr_0478 at 517.367 kb on - strand, within Psyr_0478 at 517.367 kb on - strand, within Psyr_0478 at 517.367 kb on - strand, within Psyr_0478 at 517.367 kb on - strand, within Psyr_0478 at 517.367 kb on - strand, within Psyr_0478
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 514,746 + Psyr_0475 0.81 -0.2 514,746 + Psyr_0475 0.81 -1.0 514,746 + Psyr_0475 0.81 +0.9 514,746 + Psyr_0475 0.81 -2.2 514,746 + Psyr_0475 0.81 -2.4 514,746 + Psyr_0475 0.81 +0.3 514,787 + Psyr_0475 0.88 -1.0 514,787 + Psyr_0475 0.88 -1.3 514,787 + Psyr_0475 0.88 -0.8 514,788 - Psyr_0475 0.88 -1.5 514,800 + -0.2 514,828 - -0.1 514,828 - -1.8 514,828 - -1.8 514,828 - +0.8 514,828 - -0.2 515,167 + Psyr_0476 0.35 +1.2 515,496 + Psyr_0476 0.75 +0.9 515,734 + -1.3 515,996 + Psyr_0477 0.40 +0.8 516,117 + Psyr_0477 0.58 -0.2 516,117 + Psyr_0477 0.58 -0.3 516,118 - Psyr_0477 0.58 +0.2 516,236 - Psyr_0477 0.75 -0.2 516,268 + Psyr_0477 0.80 +0.7 516,268 + Psyr_0477 0.80 +1.8 516,268 + Psyr_0477 0.80 +1.2 516,268 + Psyr_0477 0.80 -0.7 516,268 + Psyr_0477 0.80 +2.7 516,268 + Psyr_0477 0.80 +1.9 516,268 + Psyr_0477 0.80 +0.5 516,269 - Psyr_0477 0.80 +1.3 516,269 - Psyr_0477 0.80 +0.5 516,269 - Psyr_0477 0.80 +1.5 516,269 - Psyr_0477 0.80 -3.1 516,269 - Psyr_0477 0.80 +0.1 516,269 - Psyr_0477 0.80 +0.8 516,269 - Psyr_0477 0.80 +0.9 516,269 - Psyr_0477 0.80 +0.1 516,269 - Psyr_0477 0.80 -1.2 516,269 - Psyr_0477 0.80 -1.8 516,269 - Psyr_0477 0.80 +0.0 516,269 - Psyr_0477 0.80 +0.1 516,302 + Psyr_0477 0.85 +3.2 516,444 + +0.1 516,444 + -0.1 516,444 + -0.9 516,444 + +0.8 516,444 + +0.5 516,444 + -1.2 516,445 - -1.7 516,445 - -0.2 516,445 - +0.3 516,445 - +0.5 516,445 - -2.0 516,445 - -0.7 516,445 - +0.8 516,445 - -0.0 516,445 - -1.1 516,445 - +0.2 516,445 - +0.9 516,462 + -0.7 516,463 - +0.1 516,463 - +0.9 516,469 - +1.2 516,570 + Psyr_0478 0.10 -0.2 516,616 + Psyr_0478 0.15 +0.6 516,616 + Psyr_0478 0.15 +1.1 516,616 + Psyr_0478 0.15 -0.2 516,616 + Psyr_0478 0.15 +2.9 516,617 - Psyr_0478 0.15 +1.0 516,617 - Psyr_0478 0.15 +1.3 516,617 - Psyr_0478 0.15 +0.2 516,617 - Psyr_0478 0.15 -0.4 516,817 - Psyr_0478 0.34 -0.3 516,898 + Psyr_0478 0.42 +0.1 516,899 - Psyr_0478 0.42 +0.9 516,899 - Psyr_0478 0.42 -0.6 516,906 + Psyr_0478 0.43 +0.5 516,912 + Psyr_0478 0.43 +1.3 516,912 + Psyr_0478 0.43 -0.4 516,912 + Psyr_0478 0.43 +0.6 516,912 + Psyr_0478 0.43 +0.1 516,913 - Psyr_0478 0.43 -0.2 516,924 + Psyr_0478 0.45 +0.2 516,924 + Psyr_0478 0.45 -0.0 516,924 + Psyr_0478 0.45 +1.0 516,925 - Psyr_0478 0.45 +1.3 516,925 - Psyr_0478 0.45 -1.2 516,925 - Psyr_0478 0.45 +1.6 516,925 - Psyr_0478 0.45 +0.9 517,133 + Psyr_0478 0.65 +0.1 517,366 + Psyr_0478 0.87 -0.5 517,366 + Psyr_0478 0.87 -0.5 517,366 + Psyr_0478 0.87 +2.6 517,366 + Psyr_0478 0.87 -0.5 517,366 + Psyr_0478 0.87 +0.9 517,367 - Psyr_0478 0.87 +0.1 517,367 - Psyr_0478 0.87 +2.1 517,367 - Psyr_0478 0.87 -0.2 517,367 - Psyr_0478 0.87 +0.5 517,367 - Psyr_0478 0.87 +0.1 517,367 - Psyr_0478 0.87 -1.2
Or see this region's nucleotide sequence