Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0432 and Psyr_0433 overlap by 4 nucleotides Psyr_0433 and Psyr_0434 overlap by 8 nucleotides Psyr_0434 and Psyr_0435 are separated by 1 nucleotides Psyr_0435 and Psyr_0436 overlap by 4 nucleotides Psyr_0436 and Psyr_0437 are separated by 9 nucleotides
Psyr_0432: Psyr_0432 - conserved hypothetical protein, at 471,117 to 472,064
_0432
Psyr_0433: Psyr_0433 - Glycosyl transferase, family 2, at 472,061 to 472,795
_0433
Psyr_0434: Psyr_0434 - AMP-dependent synthetase and ligase, at 472,788 to 474,479
_0434
Psyr_0435: Psyr_0435 - membrane protein, putative, at 474,481 to 475,026
_0435
Psyr_0436: Psyr_0436 - Phosphopantetheine-binding protein, at 475,023 to 475,277
_0436
Psyr_0437: Psyr_0437 - acyl carrier protein, putative, at 475,287 to 475,547
_0437
Position (kb) 472
473
474
475 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 471.878 kb on - strand, within Psyr_0432 at 471.923 kb on - strand, within Psyr_0432 at 471.923 kb on - strand, within Psyr_0432 at 471.923 kb on - strand, within Psyr_0432 at 471.937 kb on + strand, within Psyr_0432 at 472.192 kb on - strand, within Psyr_0433 at 472.399 kb on - strand, within Psyr_0433 at 472.407 kb on + strand, within Psyr_0433 at 472.408 kb on - strand, within Psyr_0433 at 472.629 kb on + strand, within Psyr_0433 at 472.629 kb on + strand, within Psyr_0433 at 472.630 kb on - strand, within Psyr_0433 at 472.630 kb on - strand, within Psyr_0433 at 472.630 kb on - strand, within Psyr_0433 at 472.630 kb on - strand, within Psyr_0433 at 472.630 kb on - strand, within Psyr_0433 at 472.634 kb on - strand, within Psyr_0433 at 472.658 kb on + strand, within Psyr_0433 at 472.789 kb on + strand at 472.789 kb on + strand at 472.789 kb on + strand at 472.789 kb on + strand at 472.789 kb on + strand at 472.789 kb on + strand at 472.789 kb on + strand at 472.790 kb on - strand at 472.790 kb on - strand at 472.790 kb on - strand at 472.790 kb on - strand at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.952 kb on - strand, within Psyr_0434 at 473.952 kb on - strand, within Psyr_0434 at 473.952 kb on - strand, within Psyr_0434 at 474.661 kb on - strand, within Psyr_0435 at 474.669 kb on - strand, within Psyr_0435 at 475.049 kb on - strand, within Psyr_0436 at 475.049 kb on - strand, within Psyr_0436 at 475.059 kb on + strand, within Psyr_0436 at 475.060 kb on - strand, within Psyr_0436 at 475.460 kb on + strand, within Psyr_0437 at 475.461 kb on - strand, within Psyr_0437
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 471,878 - Psyr_0432 0.80 +1.2 471,923 - Psyr_0432 0.85 -0.9 471,923 - Psyr_0432 0.85 +1.0 471,923 - Psyr_0432 0.85 +1.6 471,937 + Psyr_0432 0.86 -0.5 472,192 - Psyr_0433 0.18 +1.8 472,399 - Psyr_0433 0.46 -1.3 472,407 + Psyr_0433 0.47 +0.0 472,408 - Psyr_0433 0.47 +0.7 472,629 + Psyr_0433 0.77 +0.7 472,629 + Psyr_0433 0.77 -0.4 472,630 - Psyr_0433 0.77 -1.0 472,630 - Psyr_0433 0.77 -1.1 472,630 - Psyr_0433 0.77 -0.3 472,630 - Psyr_0433 0.77 -1.0 472,630 - Psyr_0433 0.77 +1.4 472,634 - Psyr_0433 0.78 -1.9 472,658 + Psyr_0433 0.81 +0.4 472,789 + -0.9 472,789 + -1.6 472,789 + -0.4 472,789 + +0.0 472,789 + +0.1 472,789 + -0.0 472,789 + +0.9 472,790 - +0.2 472,790 - +0.0 472,790 - +0.6 472,790 - -0.5 473,951 + Psyr_0434 0.69 -0.1 473,951 + Psyr_0434 0.69 -0.8 473,951 + Psyr_0434 0.69 -1.4 473,951 + Psyr_0434 0.69 +1.5 473,951 + Psyr_0434 0.69 -1.0 473,951 + Psyr_0434 0.69 +1.1 473,951 + Psyr_0434 0.69 +0.1 473,951 + Psyr_0434 0.69 +0.9 473,952 - Psyr_0434 0.69 +3.9 473,952 - Psyr_0434 0.69 +1.5 473,952 - Psyr_0434 0.69 -1.8 474,661 - Psyr_0435 0.33 +0.9 474,669 - Psyr_0435 0.34 +1.0 475,049 - Psyr_0436 0.10 +0.2 475,049 - Psyr_0436 0.10 +1.2 475,059 + Psyr_0436 0.14 +0.9 475,060 - Psyr_0436 0.15 -0.3 475,460 + Psyr_0437 0.66 -0.6 475,461 - Psyr_0437 0.67 -0.5
Or see this region's nucleotide sequence