Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0381 and Psyr_0382 overlap by 4 nucleotides Psyr_0382 and Psyr_0383 overlap by 4 nucleotides Psyr_0383 and Psyr_0384 are separated by 4 nucleotides Psyr_0384 and Psyr_0385 are separated by 44 nucleotides Psyr_0385 and Psyr_0386 are separated by 2 nucleotides
Psyr_0381: Psyr_0381 - Conserved hypothetical protein 46, at 412,251 to 412,958
_0381
Psyr_0382: Psyr_0382 - Sec-independent periplasmic protein translocase, at 412,955 to 413,755
_0382
Psyr_0383: Psyr_0383 - Twin-arginine translocation protein TatB, at 413,752 to 414,228
_0383
Psyr_0384: Psyr_0384 - Twin-arginine translocation protein TatA/E, at 414,233 to 414,508
_0384
Psyr_0385: Psyr_0385 - phosphoribosyl-ATP pyrophosphatase, at 414,553 to 414,885
_0385
Psyr_0386: Psyr_0386 - phosphoribosyl-AMP cyclohydrolase, at 414,888 to 415,280
_0386
Position (kb) 413
414
415 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 413.158 kb on - strand, within Psyr_0382 at 413.617 kb on - strand, within Psyr_0382 at 413.635 kb on - strand, within Psyr_0382 at 414.028 kb on - strand, within Psyr_0383 at 414.028 kb on - strand, within Psyr_0383 at 414.028 kb on - strand, within Psyr_0383 at 414.128 kb on - strand, within Psyr_0383 at 414.459 kb on - strand, within Psyr_0384 at 414.459 kb on - strand, within Psyr_0384 at 414.693 kb on - strand, within Psyr_0385 at 414.815 kb on + strand, within Psyr_0385 at 414.815 kb on + strand, within Psyr_0385 at 414.815 kb on + strand, within Psyr_0385 at 414.816 kb on - strand, within Psyr_0385 at 414.816 kb on - strand, within Psyr_0385 at 414.816 kb on - strand, within Psyr_0385 at 414.816 kb on - strand, within Psyr_0385 at 414.816 kb on - strand, within Psyr_0385 at 414.877 kb on - strand at 414.877 kb on - strand at 414.877 kb on - strand at 414.877 kb on - strand at 414.877 kb on - strand at 414.905 kb on - strand at 414.905 kb on - strand at 414.905 kb on - strand at 414.909 kb on - strand at 414.909 kb on - strand at 414.909 kb on - strand at 414.909 kb on - strand at 414.909 kb on - strand at 414.909 kb on - strand at 414.909 kb on - strand at 414.909 kb on - strand at 414.937 kb on - strand, within Psyr_0386 at 415.132 kb on + strand, within Psyr_0386 at 415.133 kb on - strand, within Psyr_0386 at 415.133 kb on - strand, within Psyr_0386 at 415.133 kb on - strand, within Psyr_0386 at 415.133 kb on - strand, within Psyr_0386 at 415.133 kb on - strand, within Psyr_0386 at 415.133 kb on - strand, within Psyr_0386 at 415.133 kb on - strand, within Psyr_0386 at 415.133 kb on - strand, within Psyr_0386 at 415.133 kb on - strand, within Psyr_0386
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 413,158 - Psyr_0382 0.25 +1.4 413,617 - Psyr_0382 0.83 -1.6 413,635 - Psyr_0382 0.85 -0.2 414,028 - Psyr_0383 0.58 +1.4 414,028 - Psyr_0383 0.58 -1.2 414,028 - Psyr_0383 0.58 +3.0 414,128 - Psyr_0383 0.79 -1.2 414,459 - Psyr_0384 0.82 -0.6 414,459 - Psyr_0384 0.82 -0.6 414,693 - Psyr_0385 0.42 +2.0 414,815 + Psyr_0385 0.79 -0.2 414,815 + Psyr_0385 0.79 -0.1 414,815 + Psyr_0385 0.79 +4.0 414,816 - Psyr_0385 0.79 -0.6 414,816 - Psyr_0385 0.79 -0.5 414,816 - Psyr_0385 0.79 -0.7 414,816 - Psyr_0385 0.79 -0.2 414,816 - Psyr_0385 0.79 -2.8 414,877 - +0.3 414,877 - -0.3 414,877 - -1.4 414,877 - +0.5 414,877 - +0.3 414,905 - +1.2 414,905 - +1.4 414,905 - -1.4 414,909 - +0.5 414,909 - +0.3 414,909 - -0.9 414,909 - +1.1 414,909 - +1.5 414,909 - +0.8 414,909 - -0.6 414,909 - +2.0 414,937 - Psyr_0386 0.12 +0.7 415,132 + Psyr_0386 0.62 +1.4 415,133 - Psyr_0386 0.62 +0.0 415,133 - Psyr_0386 0.62 -2.8 415,133 - Psyr_0386 0.62 +2.0 415,133 - Psyr_0386 0.62 +3.5 415,133 - Psyr_0386 0.62 -1.2 415,133 - Psyr_0386 0.62 +1.4 415,133 - Psyr_0386 0.62 -2.1 415,133 - Psyr_0386 0.62 -0.9 415,133 - Psyr_0386 0.62 +1.7
Or see this region's nucleotide sequence