Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0346 and Psyr_0347 are separated by 59 nucleotides Psyr_0347 and Psyr_0348 are separated by 137 nucleotides Psyr_0348 and Psyr_0349 are separated by 56 nucleotides Psyr_0349 and Psyr_0350 overlap by 11 nucleotides
Psyr_0346: Psyr_0346 - Acriflavin resistance protein, at 368,978 to 372,079
_0346
Psyr_0347: Psyr_0347 - hypothetical protein, at 372,139 to 372,636
_0347
Psyr_0348: Psyr_0348 - conserved hypothetical protein, at 372,774 to 373,400
_0348
Psyr_0349: Psyr_0349 - Binding-protein-dependent transport systems inner membrane component, at 373,457 to 374,101
_0349
Psyr_0350: Psyr_0350 - ABC transporter, at 374,091 to 375,221
_0350
Position (kb) 372
373
374 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 371.780 kb on + strand at 371.780 kb on + strand at 371.781 kb on - strand at 371.781 kb on - strand at 371.781 kb on - strand at 371.781 kb on - strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.985 kb on - strand at 371.985 kb on - strand at 371.985 kb on - strand at 372.199 kb on + strand, within Psyr_0347 at 372.247 kb on - strand, within Psyr_0347 at 372.386 kb on + strand, within Psyr_0347 at 372.400 kb on + strand, within Psyr_0347 at 372.400 kb on + strand, within Psyr_0347 at 372.402 kb on + strand, within Psyr_0347 at 372.457 kb on - strand, within Psyr_0347 at 372.519 kb on + strand, within Psyr_0347 at 372.623 kb on + strand at 372.623 kb on + strand at 372.623 kb on + strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.681 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.690 kb on - strand at 372.690 kb on - strand at 372.690 kb on - strand at 372.690 kb on - strand at 372.708 kb on + strand at 372.708 kb on + strand at 372.708 kb on + strand at 372.709 kb on - strand at 372.709 kb on - strand at 372.749 kb on + strand at 372.749 kb on + strand at 372.750 kb on - strand at 372.750 kb on - strand at 372.750 kb on - strand at 372.750 kb on - strand at 372.838 kb on + strand, within Psyr_0348 at 372.839 kb on - strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.905 kb on - strand, within Psyr_0348 at 373.287 kb on - strand, within Psyr_0348 at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.432 kb on + strand at 373.432 kb on + strand at 373.432 kb on + strand at 373.432 kb on + strand at 373.434 kb on + strand at 373.435 kb on - strand at 373.852 kb on - strand, within Psyr_0349 at 373.971 kb on + strand, within Psyr_0349 at 373.971 kb on + strand, within Psyr_0349 at 373.971 kb on + strand, within Psyr_0349 at 373.972 kb on - strand, within Psyr_0349 at 373.972 kb on - strand, within Psyr_0349 at 374.101 kb on + strand at 374.102 kb on - strand at 374.102 kb on - strand at 374.110 kb on - strand at 374.148 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 371,780 + +1.4 371,780 + -0.1 371,781 - -0.9 371,781 - +1.6 371,781 - -2.7 371,781 - -0.1 371,984 + -0.9 371,984 + +1.0 371,984 + -0.7 371,984 + +1.1 371,984 + +0.6 371,984 + -0.2 371,985 - -1.9 371,985 - -0.2 371,985 - +0.3 372,199 + Psyr_0347 0.12 -1.1 372,247 - Psyr_0347 0.22 -0.4 372,386 + Psyr_0347 0.50 -0.3 372,400 + Psyr_0347 0.52 -0.7 372,400 + Psyr_0347 0.52 +0.2 372,402 + Psyr_0347 0.53 +0.3 372,457 - Psyr_0347 0.64 +0.5 372,519 + Psyr_0347 0.76 -0.2 372,623 + +2.0 372,623 + +0.0 372,623 + -0.4 372,624 - +0.4 372,624 - +0.8 372,624 - -1.8 372,624 - +0.7 372,624 - -2.1 372,624 - -1.4 372,624 - -0.3 372,624 - -0.6 372,624 - +0.9 372,624 - -0.3 372,624 - -1.4 372,624 - -0.5 372,624 - -1.0 372,624 - -0.2 372,624 - +0.4 372,681 + -0.1 372,689 + +0.9 372,689 + +0.9 372,689 + -0.6 372,689 + +0.9 372,689 + -0.3 372,689 + -0.7 372,689 + +0.6 372,689 + +0.4 372,689 + +0.2 372,690 - -1.2 372,690 - +1.4 372,690 - +0.5 372,690 - -0.6 372,708 + -0.7 372,708 + -1.5 372,708 + +0.4 372,709 - +0.8 372,709 - +2.0 372,749 + +0.8 372,749 + -1.9 372,750 - +2.0 372,750 - +0.2 372,750 - +2.5 372,750 - -0.5 372,838 + Psyr_0348 0.10 +0.0 372,839 - Psyr_0348 0.10 +1.1 372,841 + Psyr_0348 0.11 -0.4 372,841 + Psyr_0348 0.11 +0.4 372,841 + Psyr_0348 0.11 +1.1 372,841 + Psyr_0348 0.11 +1.4 372,842 - Psyr_0348 0.11 +1.5 372,842 - Psyr_0348 0.11 +0.5 372,842 - Psyr_0348 0.11 +0.3 372,842 - Psyr_0348 0.11 +0.6 372,905 - Psyr_0348 0.21 +1.0 373,287 - Psyr_0348 0.82 -0.6 373,345 + -0.6 373,345 + +1.2 373,345 + +0.7 373,345 + -0.2 373,345 + +0.1 373,345 + +0.6 373,346 - +3.4 373,346 - -0.3 373,346 - -1.0 373,346 - -0.0 373,346 - -2.2 373,346 - -1.4 373,432 + -0.8 373,432 + +0.1 373,432 + +1.3 373,432 + +1.2 373,434 + -0.4 373,435 - -0.2 373,852 - Psyr_0349 0.61 -1.2 373,971 + Psyr_0349 0.80 -0.1 373,971 + Psyr_0349 0.80 -0.2 373,971 + Psyr_0349 0.80 +0.3 373,972 - Psyr_0349 0.80 +0.6 373,972 - Psyr_0349 0.80 -1.7 374,101 + +2.1 374,102 - -0.3 374,102 - -0.5 374,110 - -0.3 374,148 + +1.1
Or see this region's nucleotide sequence