Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0295 and Psyr_0296 overlap by 14 nucleotides Psyr_0296 and Psyr_0297 overlap by 1 nucleotides Psyr_0297 and Psyr_0298 are separated by 31 nucleotides
Psyr_0295: Psyr_0295 - amino acid ABC transporter ATP-binding protein, PAAT family, at 315,811 to 316,545
_0295
Psyr_0296: Psyr_0296 - amino acid ABC transporter membrane protein 2, PAAT family, at 316,532 to 317,179
_0296
Psyr_0297: Psyr_0297 - amino acid ABC transporter membrane protein 1, PAAT family, at 317,179 to 317,844
_0297
Psyr_0298: Psyr_0298 - amino acid ABC transporter substrate-binding protein, PAAT family, at 317,876 to 318,661
_0298
Position (kb)
317
318 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 316.349 kb on + strand, within Psyr_0295 at 316.349 kb on + strand, within Psyr_0295 at 316.474 kb on + strand at 316.474 kb on + strand at 316.508 kb on + strand at 316.508 kb on + strand at 316.509 kb on - strand at 316.509 kb on - strand at 316.511 kb on + strand at 316.511 kb on + strand at 316.511 kb on + strand at 316.511 kb on + strand at 316.511 kb on + strand at 316.511 kb on + strand at 316.511 kb on + strand at 316.511 kb on + strand at 316.511 kb on + strand at 316.511 kb on + strand at 316.511 kb on + strand at 316.512 kb on - strand at 316.512 kb on - strand at 316.512 kb on - strand at 316.512 kb on - strand at 316.512 kb on - strand at 316.512 kb on - strand at 316.512 kb on - strand at 316.515 kb on + strand at 316.515 kb on + strand at 316.515 kb on + strand at 316.515 kb on + strand at 316.516 kb on - strand at 316.516 kb on - strand at 316.516 kb on - strand at 316.516 kb on - strand at 316.516 kb on - strand at 316.516 kb on - strand at 316.516 kb on - strand at 316.516 kb on - strand at 316.516 kb on - strand at 316.516 kb on - strand at 316.516 kb on - strand at 316.587 kb on + strand at 316.588 kb on - strand at 316.602 kb on + strand, within Psyr_0296 at 316.602 kb on + strand, within Psyr_0296 at 316.623 kb on + strand, within Psyr_0296 at 316.624 kb on - strand, within Psyr_0296 at 316.624 kb on - strand, within Psyr_0296 at 316.785 kb on + strand, within Psyr_0296 at 316.786 kb on - strand, within Psyr_0296 at 316.885 kb on - strand, within Psyr_0296 at 316.885 kb on - strand, within Psyr_0296 at 316.885 kb on - strand, within Psyr_0296 at 316.895 kb on - strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.012 kb on + strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.013 kb on - strand, within Psyr_0296 at 317.014 kb on + strand, within Psyr_0296 at 317.015 kb on - strand, within Psyr_0296 at 317.015 kb on - strand, within Psyr_0296 at 317.015 kb on - strand, within Psyr_0296 at 317.015 kb on - strand, within Psyr_0296 at 317.016 kb on + strand, within Psyr_0296 at 317.016 kb on + strand, within Psyr_0296 at 317.016 kb on + strand, within Psyr_0296 at 317.016 kb on + strand, within Psyr_0296 at 317.016 kb on + strand, within Psyr_0296 at 317.016 kb on + strand, within Psyr_0296 at 317.016 kb on + strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.017 kb on - strand, within Psyr_0296 at 317.198 kb on + strand at 317.198 kb on + strand at 317.198 kb on + strand at 317.198 kb on + strand at 317.199 kb on - strand at 317.199 kb on - strand at 317.199 kb on - strand at 317.199 kb on - strand at 317.252 kb on + strand, within Psyr_0297 at 317.253 kb on - strand, within Psyr_0297 at 317.253 kb on - strand, within Psyr_0297 at 317.253 kb on - strand, within Psyr_0297 at 317.273 kb on + strand, within Psyr_0297 at 317.273 kb on + strand, within Psyr_0297 at 317.273 kb on + strand, within Psyr_0297 at 317.273 kb on + strand, within Psyr_0297 at 317.273 kb on + strand, within Psyr_0297 at 317.273 kb on + strand, within Psyr_0297 at 317.274 kb on - strand, within Psyr_0297 at 317.274 kb on - strand, within Psyr_0297 at 317.274 kb on - strand, within Psyr_0297 at 317.274 kb on - strand, within Psyr_0297 at 317.274 kb on - strand, within Psyr_0297 at 317.535 kb on - strand, within Psyr_0297 at 317.646 kb on + strand, within Psyr_0297 at 317.646 kb on + strand, within Psyr_0297 at 317.646 kb on + strand, within Psyr_0297 at 317.646 kb on + strand, within Psyr_0297 at 317.647 kb on - strand, within Psyr_0297 at 317.647 kb on - strand, within Psyr_0297 at 317.647 kb on - strand, within Psyr_0297 at 317.647 kb on - strand, within Psyr_0297 at 317.666 kb on + strand, within Psyr_0297 at 317.666 kb on + strand, within Psyr_0297 at 317.666 kb on + strand, within Psyr_0297 at 317.666 kb on + strand, within Psyr_0297 at 317.666 kb on + strand, within Psyr_0297 at 317.666 kb on + strand, within Psyr_0297 at 317.666 kb on + strand, within Psyr_0297 at 317.666 kb on + strand, within Psyr_0297 at 317.666 kb on + strand, within Psyr_0297 at 317.666 kb on + strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.667 kb on - strand, within Psyr_0297 at 317.755 kb on + strand, within Psyr_0297 at 317.755 kb on + strand, within Psyr_0297 at 317.755 kb on + strand, within Psyr_0297 at 317.756 kb on - strand, within Psyr_0297 at 317.757 kb on + strand, within Psyr_0297 at 317.933 kb on - strand at 318.009 kb on + strand, within Psyr_0298 at 318.009 kb on + strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.126 kb on + strand, within Psyr_0298 at 318.232 kb on + strand, within Psyr_0298 at 318.232 kb on + strand, within Psyr_0298 at 318.232 kb on + strand, within Psyr_0298 at 318.233 kb on - strand, within Psyr_0298 at 318.233 kb on - strand, within Psyr_0298 at 318.297 kb on + strand, within Psyr_0298 at 318.298 kb on - strand, within Psyr_0298 at 318.298 kb on - strand, within Psyr_0298 at 318.298 kb on - strand, within Psyr_0298 at 318.298 kb on - strand, within Psyr_0298 at 318.309 kb on + strand, within Psyr_0298 at 318.472 kb on + strand, within Psyr_0298 at 318.635 kb on - strand at 318.635 kb on - strand at 318.661 kb on + strand at 318.661 kb on + strand at 318.662 kb on - strand at 318.662 kb on - strand at 318.662 kb on - strand at 318.662 kb on - strand at 318.762 kb on + strand at 318.763 kb on - strand at 318.818 kb on + strand at 318.819 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 316,349 + Psyr_0295 0.73 +0.4 316,349 + Psyr_0295 0.73 +0.2 316,474 + -0.6 316,474 + +0.2 316,508 + -0.6 316,508 + +0.3 316,509 - +0.6 316,509 - -0.3 316,511 + +0.1 316,511 + +0.1 316,511 + +2.4 316,511 + -1.5 316,511 + +0.9 316,511 + -0.3 316,511 + -0.3 316,511 + +0.4 316,511 + +0.1 316,511 + +0.9 316,511 + +1.9 316,512 - -0.8 316,512 - +2.5 316,512 - +1.0 316,512 - +1.2 316,512 - -0.7 316,512 - +0.1 316,512 - -1.1 316,515 + -1.2 316,515 + -4.0 316,515 + +0.4 316,515 + +2.7 316,516 - -0.3 316,516 - -1.6 316,516 - -0.4 316,516 - -1.3 316,516 - +0.0 316,516 - -0.4 316,516 - -0.4 316,516 - -1.9 316,516 - -0.9 316,516 - -0.5 316,516 - -1.3 316,587 + -1.8 316,588 - -1.0 316,602 + Psyr_0296 0.11 +1.4 316,602 + Psyr_0296 0.11 -1.4 316,623 + Psyr_0296 0.14 -0.9 316,624 - Psyr_0296 0.14 +0.6 316,624 - Psyr_0296 0.14 -0.2 316,785 + Psyr_0296 0.39 -0.7 316,786 - Psyr_0296 0.39 -0.7 316,885 - Psyr_0296 0.54 +0.3 316,885 - Psyr_0296 0.54 -1.3 316,885 - Psyr_0296 0.54 -1.1 316,895 - Psyr_0296 0.56 -0.1 317,012 + Psyr_0296 0.74 -0.6 317,012 + Psyr_0296 0.74 +0.8 317,012 + Psyr_0296 0.74 +0.5 317,012 + Psyr_0296 0.74 -0.1 317,012 + Psyr_0296 0.74 -1.2 317,012 + Psyr_0296 0.74 -2.2 317,012 + Psyr_0296 0.74 +0.8 317,012 + Psyr_0296 0.74 -3.1 317,012 + Psyr_0296 0.74 -0.8 317,012 + Psyr_0296 0.74 -0.6 317,012 + Psyr_0296 0.74 -0.6 317,012 + Psyr_0296 0.74 -0.0 317,012 + Psyr_0296 0.74 +0.5 317,012 + Psyr_0296 0.74 +0.2 317,012 + Psyr_0296 0.74 +0.6 317,012 + Psyr_0296 0.74 +1.3 317,012 + Psyr_0296 0.74 -1.4 317,012 + Psyr_0296 0.74 -0.7 317,013 - Psyr_0296 0.74 +0.8 317,013 - Psyr_0296 0.74 +2.2 317,013 - Psyr_0296 0.74 +0.8 317,013 - Psyr_0296 0.74 -0.4 317,013 - Psyr_0296 0.74 +0.8 317,013 - Psyr_0296 0.74 -1.3 317,013 - Psyr_0296 0.74 +0.6 317,013 - Psyr_0296 0.74 +1.3 317,013 - Psyr_0296 0.74 -1.9 317,013 - Psyr_0296 0.74 +1.4 317,013 - Psyr_0296 0.74 -1.2 317,013 - Psyr_0296 0.74 -1.6 317,013 - Psyr_0296 0.74 +0.9 317,013 - Psyr_0296 0.74 -0.9 317,014 + Psyr_0296 0.74 -0.6 317,015 - Psyr_0296 0.75 -0.7 317,015 - Psyr_0296 0.75 -0.2 317,015 - Psyr_0296 0.75 -0.0 317,015 - Psyr_0296 0.75 +0.1 317,016 + Psyr_0296 0.75 +1.4 317,016 + Psyr_0296 0.75 -1.6 317,016 + Psyr_0296 0.75 +1.4 317,016 + Psyr_0296 0.75 -0.4 317,016 + Psyr_0296 0.75 -2.4 317,016 + Psyr_0296 0.75 +1.1 317,016 + Psyr_0296 0.75 -2.3 317,017 - Psyr_0296 0.75 +0.4 317,017 - Psyr_0296 0.75 +0.1 317,017 - Psyr_0296 0.75 -0.2 317,017 - Psyr_0296 0.75 -1.5 317,017 - Psyr_0296 0.75 -1.6 317,017 - Psyr_0296 0.75 +1.4 317,017 - Psyr_0296 0.75 +0.7 317,017 - Psyr_0296 0.75 -0.3 317,017 - Psyr_0296 0.75 -0.6 317,017 - Psyr_0296 0.75 -0.6 317,017 - Psyr_0296 0.75 -0.1 317,017 - Psyr_0296 0.75 -0.2 317,017 - Psyr_0296 0.75 +0.5 317,017 - Psyr_0296 0.75 +0.0 317,017 - Psyr_0296 0.75 -0.8 317,198 + +1.4 317,198 + +0.0 317,198 + -0.3 317,198 + -0.2 317,199 - -0.1 317,199 - -0.4 317,199 - -0.7 317,199 - -1.9 317,252 + Psyr_0297 0.11 +1.2 317,253 - Psyr_0297 0.11 -0.8 317,253 - Psyr_0297 0.11 +0.2 317,253 - Psyr_0297 0.11 -0.8 317,273 + Psyr_0297 0.14 -0.2 317,273 + Psyr_0297 0.14 -0.4 317,273 + Psyr_0297 0.14 +1.4 317,273 + Psyr_0297 0.14 -1.3 317,273 + Psyr_0297 0.14 +0.3 317,273 + Psyr_0297 0.14 +0.8 317,274 - Psyr_0297 0.14 -0.6 317,274 - Psyr_0297 0.14 +0.7 317,274 - Psyr_0297 0.14 -0.2 317,274 - Psyr_0297 0.14 +1.0 317,274 - Psyr_0297 0.14 -1.6 317,535 - Psyr_0297 0.53 -0.5 317,646 + Psyr_0297 0.70 +0.7 317,646 + Psyr_0297 0.70 +0.3 317,646 + Psyr_0297 0.70 -0.4 317,646 + Psyr_0297 0.70 -2.7 317,647 - Psyr_0297 0.70 +1.0 317,647 - Psyr_0297 0.70 -0.3 317,647 - Psyr_0297 0.70 +1.5 317,647 - Psyr_0297 0.70 -0.1 317,666 + Psyr_0297 0.73 +0.8 317,666 + Psyr_0297 0.73 +0.5 317,666 + Psyr_0297 0.73 +0.6 317,666 + Psyr_0297 0.73 +0.5 317,666 + Psyr_0297 0.73 +1.9 317,666 + Psyr_0297 0.73 -1.8 317,666 + Psyr_0297 0.73 -0.1 317,666 + Psyr_0297 0.73 +0.0 317,666 + Psyr_0297 0.73 +0.8 317,666 + Psyr_0297 0.73 -0.2 317,667 - Psyr_0297 0.73 +0.9 317,667 - Psyr_0297 0.73 -1.4 317,667 - Psyr_0297 0.73 +1.1 317,667 - Psyr_0297 0.73 +0.6 317,667 - Psyr_0297 0.73 +0.4 317,667 - Psyr_0297 0.73 +0.7 317,667 - Psyr_0297 0.73 -0.9 317,667 - Psyr_0297 0.73 +0.5 317,667 - Psyr_0297 0.73 -0.6 317,667 - Psyr_0297 0.73 -1.4 317,667 - Psyr_0297 0.73 +0.3 317,667 - Psyr_0297 0.73 +0.7 317,667 - Psyr_0297 0.73 -1.6 317,667 - Psyr_0297 0.73 +0.1 317,667 - Psyr_0297 0.73 -0.2 317,667 - Psyr_0297 0.73 -0.1 317,667 - Psyr_0297 0.73 +0.8 317,755 + Psyr_0297 0.86 -0.2 317,755 + Psyr_0297 0.86 +2.3 317,755 + Psyr_0297 0.86 -0.3 317,756 - Psyr_0297 0.87 +0.4 317,757 + Psyr_0297 0.87 -1.5 317,933 - -0.9 318,009 + Psyr_0298 0.17 +1.8 318,009 + Psyr_0298 0.17 -0.8 318,010 - Psyr_0298 0.17 -0.2 318,010 - Psyr_0298 0.17 +0.0 318,010 - Psyr_0298 0.17 -1.9 318,010 - Psyr_0298 0.17 -1.3 318,010 - Psyr_0298 0.17 -2.2 318,010 - Psyr_0298 0.17 +2.1 318,010 - Psyr_0298 0.17 -0.6 318,126 + Psyr_0298 0.32 -3.0 318,232 + Psyr_0298 0.45 -1.4 318,232 + Psyr_0298 0.45 +1.6 318,232 + Psyr_0298 0.45 -0.8 318,233 - Psyr_0298 0.45 -0.9 318,233 - Psyr_0298 0.45 -0.3 318,297 + Psyr_0298 0.54 +0.7 318,298 - Psyr_0298 0.54 -1.9 318,298 - Psyr_0298 0.54 +0.9 318,298 - Psyr_0298 0.54 -2.5 318,298 - Psyr_0298 0.54 -1.0 318,309 + Psyr_0298 0.55 +0.6 318,472 + Psyr_0298 0.76 -0.2 318,635 - -0.2 318,635 - +0.0 318,661 + +0.7 318,661 + -0.1 318,662 - +0.6 318,662 - -0.2 318,662 - -0.2 318,662 - +0.3 318,762 + -2.5 318,763 - -1.6 318,818 + +0.2 318,819 - +1.4
Or see this region's nucleotide sequence