Experiment: KB with Ginkgolide A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0218 and Psyr_0219 are separated by 28 nucleotides
Psyr_0218: Psyr_0218 - N-acetylglutamate kinase, at 231,075 to 231,980
_0218
Psyr_0219: Psyr_0219 - phosphomannomutase, at 232,009 to 233,406
_0219
Position (kb)
232
233
234 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 231.043 kb on - strand at 231.065 kb on + strand at 231.066 kb on - strand at 231.109 kb on - strand at 231.109 kb on - strand at 231.920 kb on - strand at 232.291 kb on - strand, within Psyr_0219 at 232.291 kb on - strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.375 kb on - strand, within Psyr_0219 at 232.375 kb on - strand, within Psyr_0219 at 232.375 kb on - strand, within Psyr_0219 at 232.925 kb on + strand, within Psyr_0219 at 232.926 kb on - strand, within Psyr_0219 at 233.060 kb on + strand, within Psyr_0219 at 233.060 kb on + strand, within Psyr_0219 at 233.060 kb on + strand, within Psyr_0219 at 233.060 kb on + strand, within Psyr_0219 at 233.060 kb on + strand, within Psyr_0219 at 233.060 kb on + strand, within Psyr_0219 at 233.061 kb on - strand, within Psyr_0219 at 233.061 kb on - strand, within Psyr_0219 at 233.061 kb on - strand, within Psyr_0219 at 233.126 kb on + strand, within Psyr_0219 at 233.127 kb on - strand, within Psyr_0219 at 233.127 kb on - strand, within Psyr_0219 at 233.127 kb on - strand, within Psyr_0219 at 233.127 kb on - strand, within Psyr_0219 at 233.127 kb on - strand, within Psyr_0219 at 233.129 kb on + strand, within Psyr_0219 at 233.129 kb on + strand, within Psyr_0219 at 233.130 kb on - strand, within Psyr_0219 at 233.155 kb on + strand, within Psyr_0219 at 233.155 kb on + strand, within Psyr_0219 at 233.155 kb on + strand, within Psyr_0219 at 233.155 kb on + strand, within Psyr_0219 at 233.155 kb on + strand, within Psyr_0219 at 233.156 kb on - strand, within Psyr_0219 at 233.156 kb on - strand, within Psyr_0219 at 233.156 kb on - strand, within Psyr_0219 at 233.303 kb on + strand at 233.365 kb on - strand at 233.365 kb on - strand at 233.365 kb on - strand at 233.365 kb on - strand at 233.416 kb on - strand at 233.547 kb on + strand at 233.602 kb on - strand at 233.733 kb on + strand at 233.734 kb on - strand at 233.734 kb on - strand at 233.734 kb on - strand at 234.045 kb on + strand at 234.046 kb on - strand at 234.092 kb on + strand at 234.092 kb on + strand at 234.092 kb on + strand at 234.092 kb on + strand at 234.093 kb on - strand at 234.093 kb on - strand at 234.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ginkgolide A 10 mM remove 231,043 - +0.7 231,065 + -0.6 231,066 - -0.0 231,109 - -1.9 231,109 - -1.3 231,920 - +0.4 232,291 - Psyr_0219 0.20 -0.8 232,291 - Psyr_0219 0.20 +0.9 232,370 + Psyr_0219 0.26 -1.6 232,370 + Psyr_0219 0.26 -0.1 232,370 + Psyr_0219 0.26 -1.1 232,370 + Psyr_0219 0.26 -0.1 232,371 - Psyr_0219 0.26 -2.2 232,371 - Psyr_0219 0.26 -1.2 232,371 - Psyr_0219 0.26 -0.8 232,371 - Psyr_0219 0.26 -3.2 232,371 - Psyr_0219 0.26 +0.0 232,371 - Psyr_0219 0.26 -2.1 232,371 - Psyr_0219 0.26 -1.6 232,371 - Psyr_0219 0.26 +1.6 232,371 - Psyr_0219 0.26 -0.6 232,375 - Psyr_0219 0.26 -1.0 232,375 - Psyr_0219 0.26 -0.8 232,375 - Psyr_0219 0.26 +0.1 232,925 + Psyr_0219 0.66 -0.7 232,926 - Psyr_0219 0.66 -1.9 233,060 + Psyr_0219 0.75 -1.1 233,060 + Psyr_0219 0.75 -3.4 233,060 + Psyr_0219 0.75 -0.6 233,060 + Psyr_0219 0.75 -2.3 233,060 + Psyr_0219 0.75 -1.6 233,060 + Psyr_0219 0.75 -1.1 233,061 - Psyr_0219 0.75 -0.5 233,061 - Psyr_0219 0.75 -2.3 233,061 - Psyr_0219 0.75 -0.1 233,126 + Psyr_0219 0.80 +1.1 233,127 - Psyr_0219 0.80 -1.6 233,127 - Psyr_0219 0.80 -0.3 233,127 - Psyr_0219 0.80 -0.6 233,127 - Psyr_0219 0.80 -2.1 233,127 - Psyr_0219 0.80 -1.8 233,129 + Psyr_0219 0.80 -1.1 233,129 + Psyr_0219 0.80 -0.9 233,130 - Psyr_0219 0.80 -3.0 233,155 + Psyr_0219 0.82 -3.6 233,155 + Psyr_0219 0.82 -0.5 233,155 + Psyr_0219 0.82 -2.3 233,155 + Psyr_0219 0.82 -1.0 233,155 + Psyr_0219 0.82 -3.8 233,156 - Psyr_0219 0.82 -1.3 233,156 - Psyr_0219 0.82 -2.1 233,156 - Psyr_0219 0.82 +0.1 233,303 + -1.3 233,365 - +0.4 233,365 - -1.5 233,365 - +0.0 233,365 - -0.7 233,416 - -1.5 233,547 + +1.9 233,602 - -0.4 233,733 + +1.2 233,734 - +0.1 233,734 - -0.3 233,734 - +1.8 234,045 + +1.1 234,046 - -0.6 234,092 + +1.1 234,092 + -0.7 234,092 + -0.4 234,092 + -1.9 234,093 - +0.3 234,093 - -0.4 234,093 - +2.0
Or see this region's nucleotide sequence