Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS17475

Experiment: Octanoic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS17470 and ABZR87_RS17475 are separated by 19 nucleotidesABZR87_RS17475 and ABZR87_RS17480 overlap by 1 nucleotidesABZR87_RS17480 and ABZR87_RS17485 overlap by 4 nucleotides ABZR87_RS17470: ABZR87_RS17470 - CoA-acylating methylmalonate-semialdehyde dehydrogenase, at 279,020 to 280,567 _RS17470 ABZR87_RS17475: ABZR87_RS17475 - 3-hydroxyisobutyrate dehydrogenase, at 280,587 to 281,480 _RS17475 ABZR87_RS17480: ABZR87_RS17480 - enoyl-CoA hydratase, at 281,480 to 282,277 _RS17480 ABZR87_RS17485: ABZR87_RS17485 - enoyl-CoA hydratase/isomerase family protein, at 282,274 to 283,425 _RS17485 Position (kb) 280 281 282Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 279.773 kb on + strand, within ABZR87_RS17470at 279.773 kb on + strand, within ABZR87_RS17470at 279.773 kb on + strand, within ABZR87_RS17470at 279.774 kb on - strand, within ABZR87_RS17470at 279.774 kb on - strand, within ABZR87_RS17470at 280.200 kb on - strand, within ABZR87_RS17470at 280.205 kb on + strand, within ABZR87_RS17470at 280.205 kb on + strand, within ABZR87_RS17470at 280.205 kb on + strand, within ABZR87_RS17470at 280.205 kb on + strand, within ABZR87_RS17470at 280.206 kb on - strand, within ABZR87_RS17470at 280.206 kb on - strand, within ABZR87_RS17470at 280.252 kb on + strand, within ABZR87_RS17470at 280.406 kb on + strand, within ABZR87_RS17470at 280.481 kb on + strandat 280.547 kb on - strandat 280.683 kb on - strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.795 kb on + strand, within ABZR87_RS17475at 280.796 kb on - strand, within ABZR87_RS17475at 281.239 kb on - strand, within ABZR87_RS17475at 281.239 kb on - strand, within ABZR87_RS17475at 281.239 kb on - strand, within ABZR87_RS17475at 281.286 kb on + strand, within ABZR87_RS17475at 281.370 kb on + strand, within ABZR87_RS17475at 281.370 kb on + strand, within ABZR87_RS17475at 281.458 kb on - strandat 282.366 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Octanoic (C)
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279,773 + ABZR87_RS17470 0.49 +1.4
279,773 + ABZR87_RS17470 0.49 +0.6
279,773 + ABZR87_RS17470 0.49 -2.1
279,774 - ABZR87_RS17470 0.49 +0.8
279,774 - ABZR87_RS17470 0.49 +0.2
280,200 - ABZR87_RS17470 0.76 +2.3
280,205 + ABZR87_RS17470 0.77 -0.9
280,205 + ABZR87_RS17470 0.77 +1.1
280,205 + ABZR87_RS17470 0.77 -1.4
280,205 + ABZR87_RS17470 0.77 +2.7
280,206 - ABZR87_RS17470 0.77 +3.7
280,206 - ABZR87_RS17470 0.77 +0.6
280,252 + ABZR87_RS17470 0.80 +3.2
280,406 + ABZR87_RS17470 0.90 +1.6
280,481 + +2.3
280,547 - +0.4
280,683 - ABZR87_RS17475 0.11 +1.2
280,795 + ABZR87_RS17475 0.23 +3.3
280,795 + ABZR87_RS17475 0.23 +1.5
280,795 + ABZR87_RS17475 0.23 -2.9
280,795 + ABZR87_RS17475 0.23 -0.5
280,795 + ABZR87_RS17475 0.23 +0.9
280,795 + ABZR87_RS17475 0.23 +2.0
280,795 + ABZR87_RS17475 0.23 +0.3
280,796 - ABZR87_RS17475 0.23 -0.6
281,239 - ABZR87_RS17475 0.73 -0.4
281,239 - ABZR87_RS17475 0.73 -0.5
281,239 - ABZR87_RS17475 0.73 -2.0
281,286 + ABZR87_RS17475 0.78 +2.6
281,370 + ABZR87_RS17475 0.88 +3.2
281,370 + ABZR87_RS17475 0.88 -1.4
281,458 - +1.7
282,366 + -1.4

Or see this region's nucleotide sequence