Experiment: BHIS with Ramoplanin 0.003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2642 and BT2643 are separated by 46 nucleotides BT2643 and BT2644 are separated by 94 nucleotides BT2644 and BT2645 are separated by 745 nucleotides
BT2642: BT2642 - conserved hypothetical protein (NCBI ptt file), at 3,293,837 to 3,295,642
BT2642
BT2643: BT2643 - conserved hypothetical protein (NCBI ptt file), at 3,295,689 to 3,295,964
BT2643
BT2644: BT2644 - DNA topoisomerase I (NCBI ptt file), at 3,296,059 to 3,297,819
BT2644
BT2645: BT2645 - hypothetical protein (NCBI ptt file), at 3,298,565 to 3,299,647
BT2645
Position (kb)
3296
3297
3298 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3295.143 kb on - strand, within BT2642 at 3295.176 kb on - strand, within BT2642 at 3295.176 kb on - strand, within BT2642 at 3295.402 kb on - strand, within BT2642 at 3295.402 kb on - strand, within BT2642 at 3295.447 kb on - strand, within BT2642 at 3295.641 kb on - strand at 3295.862 kb on - strand, within BT2643 at 3295.963 kb on - strand at 3295.994 kb on + strand at 3296.103 kb on - strand at 3296.103 kb on - strand at 3296.419 kb on + strand, within BT2644 at 3296.463 kb on - strand, within BT2644 at 3296.478 kb on + strand, within BT2644 at 3296.530 kb on + strand, within BT2644 at 3296.673 kb on - strand, within BT2644 at 3296.813 kb on - strand, within BT2644 at 3296.876 kb on + strand, within BT2644 at 3296.877 kb on - strand, within BT2644 at 3296.894 kb on - strand, within BT2644 at 3296.894 kb on - strand, within BT2644 at 3296.929 kb on + strand, within BT2644 at 3296.929 kb on + strand, within BT2644 at 3296.930 kb on - strand, within BT2644 at 3296.930 kb on - strand, within BT2644 at 3297.038 kb on - strand, within BT2644 at 3297.141 kb on - strand, within BT2644 at 3297.189 kb on + strand, within BT2644 at 3297.194 kb on - strand, within BT2644 at 3297.230 kb on - strand, within BT2644 at 3297.230 kb on - strand, within BT2644 at 3297.230 kb on - strand, within BT2644 at 3297.273 kb on - strand, within BT2644 at 3297.286 kb on + strand, within BT2644 at 3297.287 kb on - strand, within BT2644 at 3297.306 kb on - strand, within BT2644 at 3297.500 kb on - strand, within BT2644 at 3297.502 kb on - strand, within BT2644 at 3297.595 kb on + strand, within BT2644 at 3297.595 kb on + strand, within BT2644 at 3297.595 kb on + strand, within BT2644 at 3297.596 kb on - strand, within BT2644 at 3297.599 kb on + strand, within BT2644 at 3297.600 kb on - strand, within BT2644 at 3297.600 kb on - strand, within BT2644 at 3297.604 kb on + strand, within BT2644 at 3297.616 kb on + strand, within BT2644 at 3297.616 kb on + strand, within BT2644 at 3297.616 kb on + strand, within BT2644 at 3297.643 kb on + strand, within BT2644 at 3297.644 kb on - strand at 3297.647 kb on + strand at 3297.647 kb on + strand at 3297.647 kb on + strand at 3297.647 kb on + strand at 3297.648 kb on - strand at 3297.649 kb on + strand at 3297.649 kb on + strand at 3297.649 kb on + strand at 3297.649 kb on + strand at 3297.650 kb on - strand at 3297.650 kb on - strand at 3297.650 kb on - strand at 3297.786 kb on - strand at 3297.825 kb on - strand at 3297.825 kb on - strand at 3297.827 kb on - strand at 3297.827 kb on - strand at 3297.829 kb on - strand at 3297.829 kb on - strand at 3297.831 kb on - strand at 3297.831 kb on - strand at 3297.831 kb on - strand at 3297.831 kb on - strand at 3297.831 kb on - strand at 3297.873 kb on - strand at 3298.081 kb on - strand at 3298.081 kb on - strand at 3298.081 kb on - strand at 3298.081 kb on - strand at 3298.169 kb on - strand at 3298.169 kb on - strand at 3298.169 kb on - strand at 3298.206 kb on - strand at 3298.206 kb on - strand at 3298.226 kb on - strand at 3298.238 kb on - strand at 3298.313 kb on - strand at 3298.433 kb on - strand at 3298.464 kb on - strand at 3298.524 kb on - strand at 3298.543 kb on - strand at 3298.545 kb on - strand at 3298.660 kb on - strand at 3298.670 kb on - strand at 3298.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Ramoplanin 0.003125 mM remove 3,295,143 - BT2642 0.72 +1.2 3,295,176 - BT2642 0.74 -0.8 3,295,176 - BT2642 0.74 +1.2 3,295,402 - BT2642 0.87 -0.4 3,295,402 - BT2642 0.87 -0.5 3,295,447 - BT2642 0.89 -0.8 3,295,641 - -0.2 3,295,862 - BT2643 0.63 -1.4 3,295,963 - -1.5 3,295,994 + -0.4 3,296,103 - -0.6 3,296,103 - +0.8 3,296,419 + BT2644 0.20 -2.6 3,296,463 - BT2644 0.23 -1.3 3,296,478 + BT2644 0.24 +0.2 3,296,530 + BT2644 0.27 -1.4 3,296,673 - BT2644 0.35 -0.7 3,296,813 - BT2644 0.43 -0.9 3,296,876 + BT2644 0.46 -2.6 3,296,877 - BT2644 0.46 -1.4 3,296,894 - BT2644 0.47 +2.5 3,296,894 - BT2644 0.47 -2.8 3,296,929 + BT2644 0.49 -1.4 3,296,929 + BT2644 0.49 +0.8 3,296,930 - BT2644 0.49 +0.2 3,296,930 - BT2644 0.49 -0.9 3,297,038 - BT2644 0.56 -1.9 3,297,141 - BT2644 0.61 -2.0 3,297,189 + BT2644 0.64 -2.8 3,297,194 - BT2644 0.64 -0.8 3,297,230 - BT2644 0.66 -2.3 3,297,230 - BT2644 0.66 -1.5 3,297,230 - BT2644 0.66 -0.8 3,297,273 - BT2644 0.69 -0.7 3,297,286 + BT2644 0.70 -1.6 3,297,287 - BT2644 0.70 -0.8 3,297,306 - BT2644 0.71 -2.6 3,297,500 - BT2644 0.82 -0.8 3,297,502 - BT2644 0.82 -0.6 3,297,595 + BT2644 0.87 -1.8 3,297,595 + BT2644 0.87 -1.6 3,297,595 + BT2644 0.87 -2.1 3,297,596 - BT2644 0.87 +0.2 3,297,599 + BT2644 0.87 -1.4 3,297,600 - BT2644 0.88 -2.9 3,297,600 - BT2644 0.88 -0.9 3,297,604 + BT2644 0.88 -3.0 3,297,616 + BT2644 0.88 -1.8 3,297,616 + BT2644 0.88 -4.6 3,297,616 + BT2644 0.88 -1.9 3,297,643 + BT2644 0.90 -0.8 3,297,644 - -1.4 3,297,647 + -0.9 3,297,647 + -1.6 3,297,647 + -1.4 3,297,647 + +1.2 3,297,648 - +0.2 3,297,649 + -0.9 3,297,649 + +1.2 3,297,649 + -1.3 3,297,649 + -1.4 3,297,650 - -1.8 3,297,650 - -0.9 3,297,650 - -1.8 3,297,786 - +0.2 3,297,825 - -1.4 3,297,825 - -1.8 3,297,827 - -0.9 3,297,827 - -0.5 3,297,829 - -1.8 3,297,829 - -2.4 3,297,831 - -2.1 3,297,831 - -1.7 3,297,831 - -0.6 3,297,831 - -1.2 3,297,831 - -0.5 3,297,873 - -0.8 3,298,081 - -0.8 3,298,081 - -0.8 3,298,081 - -1.8 3,298,081 - -0.7 3,298,169 - -1.1 3,298,169 - -0.4 3,298,169 - -0.8 3,298,206 - +1.2 3,298,206 - -2.0 3,298,226 - -1.4 3,298,238 - -1.4 3,298,313 - -1.8 3,298,433 - -0.3 3,298,464 - +1.8 3,298,524 - -1.0 3,298,543 - -0.9 3,298,545 - -0.2 3,298,660 - +0.2 3,298,670 - -0.7 3,298,670 - -0.5
Or see this region's nucleotide sequence