Experiment: BHIS with Ramoplanin 0.003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2028 and BT2029 are separated by 1 nucleotides BT2029 and BT2030 are separated by 365 nucleotides BT2030 and BT2031 are separated by 57 nucleotides BT2031 and BT2032 are separated by 441 nucleotides
BT2028: BT2028 - conserved hypothetical protein (NCBI ptt file), at 2,545,159 to 2,546,166
BT2028
BT2029: BT2029 - conserved hypothetical protein (NCBI ptt file), at 2,546,168 to 2,546,686
BT2029
BT2030: BT2030 - conserved hypothetical protein (NCBI ptt file), at 2,547,052 to 2,547,966
BT2030
BT2031: BT2031 - hypothetical protein (NCBI ptt file), at 2,548,024 to 2,548,413
BT2031
BT2032: BT2032 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 2,548,855 to 2,551,722
BT2032
Position (kb)
2547
2548 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2546.160 kb on - strand at 2546.164 kb on + strand at 2546.164 kb on + strand at 2546.183 kb on + strand at 2546.189 kb on + strand at 2546.203 kb on - strand at 2546.235 kb on - strand, within BT2029 at 2546.841 kb on + strand at 2546.842 kb on - strand at 2546.926 kb on - strand at 2546.949 kb on - strand at 2546.961 kb on + strand at 2547.022 kb on + strand at 2547.064 kb on - strand at 2547.328 kb on + strand, within BT2030 at 2547.398 kb on - strand, within BT2030 at 2547.437 kb on + strand, within BT2030 at 2547.456 kb on + strand, within BT2030 at 2547.578 kb on - strand, within BT2030 at 2547.578 kb on - strand, within BT2030 at 2547.579 kb on + strand, within BT2030 at 2547.584 kb on + strand, within BT2030 at 2547.584 kb on + strand, within BT2030 at 2547.588 kb on + strand, within BT2030 at 2547.672 kb on + strand, within BT2030 at 2547.673 kb on - strand, within BT2030 at 2547.695 kb on + strand, within BT2030 at 2547.695 kb on + strand, within BT2030 at 2547.696 kb on - strand, within BT2030 at 2547.703 kb on - strand, within BT2030 at 2547.970 kb on + strand at 2548.009 kb on - strand at 2548.062 kb on + strand at 2548.068 kb on - strand, within BT2031 at 2548.127 kb on + strand, within BT2031 at 2548.156 kb on + strand, within BT2031 at 2548.158 kb on + strand, within BT2031 at 2548.203 kb on + strand, within BT2031 at 2548.203 kb on + strand, within BT2031 at 2548.230 kb on + strand, within BT2031 at 2548.267 kb on + strand, within BT2031 at 2548.339 kb on + strand, within BT2031 at 2548.363 kb on - strand, within BT2031 at 2548.387 kb on - strand at 2548.399 kb on - strand at 2548.409 kb on + strand at 2548.553 kb on + strand at 2548.757 kb on + strand at 2548.758 kb on - strand at 2548.758 kb on - strand at 2548.844 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Ramoplanin 0.003125 mM remove 2,546,160 - -0.6 2,546,164 + -1.7 2,546,164 + -2.2 2,546,183 + -0.7 2,546,189 + -0.6 2,546,203 - -0.7 2,546,235 - BT2029 0.13 -1.6 2,546,841 + +0.4 2,546,842 - -1.8 2,546,926 - -0.6 2,546,949 - -1.6 2,546,961 + -1.1 2,547,022 + -0.8 2,547,064 - -2.0 2,547,328 + BT2030 0.30 -0.1 2,547,398 - BT2030 0.38 +2.2 2,547,437 + BT2030 0.42 -1.3 2,547,456 + BT2030 0.44 +1.4 2,547,578 - BT2030 0.57 +0.4 2,547,578 - BT2030 0.57 -1.3 2,547,579 + BT2030 0.58 -1.2 2,547,584 + BT2030 0.58 -0.6 2,547,584 + BT2030 0.58 -0.1 2,547,588 + BT2030 0.59 -1.6 2,547,672 + BT2030 0.68 -0.6 2,547,673 - BT2030 0.68 -2.2 2,547,695 + BT2030 0.70 -0.2 2,547,695 + BT2030 0.70 +0.2 2,547,696 - BT2030 0.70 +0.4 2,547,703 - BT2030 0.71 -3.8 2,547,970 + +2.0 2,548,009 - -0.6 2,548,062 + -1.4 2,548,068 - BT2031 0.11 -1.4 2,548,127 + BT2031 0.26 +0.8 2,548,156 + BT2031 0.34 +0.7 2,548,158 + BT2031 0.34 -2.0 2,548,203 + BT2031 0.46 -2.0 2,548,203 + BT2031 0.46 -4.1 2,548,230 + BT2031 0.53 -0.1 2,548,267 + BT2031 0.62 -1.2 2,548,339 + BT2031 0.81 +0.4 2,548,363 - BT2031 0.87 +0.4 2,548,387 - -2.0 2,548,399 - +0.0 2,548,409 + -1.6 2,548,553 + -0.5 2,548,757 + -1.4 2,548,758 - -1.2 2,548,758 - -2.0 2,548,844 + -1.2
Or see this region's nucleotide sequence