Experiment: BHIS with Ramoplanin 0.003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1003 and BT1004 are separated by 135 nucleotides BT1004 and BT1005 are separated by 413 nucleotides BT1005 and BT1006 are separated by 26 nucleotides
BT1003: BT1003 - conserved hypothetical protein (NCBI ptt file), at 1,249,524 to 1,251,620
BT1003
BT1004: BT1004 - conserved hypothetical protein (NCBI ptt file), at 1,251,756 to 1,252,529
BT1004
BT1005: BT1005 - hypothetical protein (NCBI ptt file), at 1,252,943 to 1,253,200
BT1005
BT1006: BT1006 - putative nitroreductase (NCBI ptt file), at 1,253,227 to 1,253,955
BT1006
Position (kb) 1251
1252
1253 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1250.781 kb on - strand, within BT1003 at 1250.866 kb on + strand, within BT1003 at 1250.888 kb on - strand, within BT1003 at 1250.903 kb on + strand, within BT1003 at 1250.961 kb on - strand, within BT1003 at 1251.162 kb on - strand, within BT1003 at 1251.192 kb on + strand, within BT1003 at 1251.192 kb on + strand, within BT1003 at 1251.193 kb on - strand, within BT1003 at 1251.310 kb on + strand, within BT1003 at 1251.311 kb on - strand, within BT1003 at 1251.450 kb on + strand at 1251.490 kb on - strand at 1251.626 kb on - strand at 1251.682 kb on - strand at 1251.695 kb on + strand at 1251.772 kb on + strand at 1251.925 kb on + strand, within BT1004 at 1252.176 kb on + strand, within BT1004 at 1252.188 kb on - strand, within BT1004 at 1252.188 kb on - strand, within BT1004 at 1252.290 kb on - strand, within BT1004 at 1252.291 kb on + strand, within BT1004 at 1252.316 kb on + strand, within BT1004 at 1252.317 kb on - strand, within BT1004 at 1252.319 kb on - strand, within BT1004 at 1252.413 kb on + strand, within BT1004 at 1252.413 kb on + strand, within BT1004 at 1252.417 kb on + strand, within BT1004 at 1252.417 kb on + strand, within BT1004 at 1252.461 kb on + strand at 1252.512 kb on - strand at 1252.519 kb on - strand at 1252.575 kb on - strand at 1252.636 kb on + strand at 1252.780 kb on + strand at 1252.780 kb on + strand at 1252.781 kb on - strand at 1252.783 kb on + strand at 1252.856 kb on + strand at 1252.857 kb on - strand at 1252.943 kb on - strand at 1252.973 kb on + strand, within BT1005 at 1252.991 kb on + strand, within BT1005 at 1252.996 kb on - strand, within BT1005 at 1252.998 kb on + strand, within BT1005 at 1253.024 kb on - strand, within BT1005 at 1253.195 kb on - strand at 1253.195 kb on - strand at 1253.347 kb on - strand, within BT1006 at 1253.424 kb on + strand, within BT1006 at 1253.424 kb on + strand, within BT1006 at 1253.461 kb on + strand, within BT1006 at 1253.462 kb on - strand, within BT1006 at 1253.499 kb on + strand, within BT1006
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Ramoplanin 0.003125 mM remove 1,250,781 - BT1003 0.60 +0.4 1,250,866 + BT1003 0.64 +0.4 1,250,888 - BT1003 0.65 -1.1 1,250,903 + BT1003 0.66 -0.3 1,250,961 - BT1003 0.69 -0.1 1,251,162 - BT1003 0.78 +0.4 1,251,192 + BT1003 0.80 -3.9 1,251,192 + BT1003 0.80 -1.9 1,251,193 - BT1003 0.80 +0.4 1,251,310 + BT1003 0.85 -1.1 1,251,311 - BT1003 0.85 -1.0 1,251,450 + -0.6 1,251,490 - -1.3 1,251,626 - -0.6 1,251,682 - -1.1 1,251,695 + -0.9 1,251,772 + -1.6 1,251,925 + BT1004 0.22 +0.4 1,252,176 + BT1004 0.54 -1.4 1,252,188 - BT1004 0.56 +0.4 1,252,188 - BT1004 0.56 -1.6 1,252,290 - BT1004 0.69 -1.1 1,252,291 + BT1004 0.69 -1.1 1,252,316 + BT1004 0.72 -0.6 1,252,317 - BT1004 0.72 -1.0 1,252,319 - BT1004 0.73 -3.0 1,252,413 + BT1004 0.85 +0.4 1,252,413 + BT1004 0.85 -1.7 1,252,417 + BT1004 0.85 -1.6 1,252,417 + BT1004 0.85 -1.7 1,252,461 + -1.6 1,252,512 - -0.1 1,252,519 - +0.4 1,252,575 - -0.6 1,252,636 + +0.4 1,252,780 + -0.3 1,252,780 + -1.2 1,252,781 - +0.4 1,252,783 + -0.6 1,252,856 + +0.4 1,252,857 - -0.6 1,252,943 - -2.9 1,252,973 + BT1005 0.12 -1.1 1,252,991 + BT1005 0.19 +0.4 1,252,996 - BT1005 0.21 -0.1 1,252,998 + BT1005 0.21 -1.6 1,253,024 - BT1005 0.31 -0.5 1,253,195 - -0.6 1,253,195 - -2.1 1,253,347 - BT1006 0.16 -2.4 1,253,424 + BT1006 0.27 -1.4 1,253,424 + BT1006 0.27 -1.1 1,253,461 + BT1006 0.32 -1.1 1,253,462 - BT1006 0.32 -0.1 1,253,499 + BT1006 0.37 -1.1
Or see this region's nucleotide sequence