Experiment: BHIS with Ramoplanin 0.003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0166 and BT0167 are separated by 129 nucleotides BT0167 and BT0168 overlap by 8 nucleotides BT0168 and BT0169 are separated by 217 nucleotides BT0169 and BT0170 are separated by 119 nucleotides BT0170 and BT0171 are separated by 171 nucleotides
BT0166: BT0166 - putative thiol-disulfide isomerase (NCBI ptt file), at 175,872 to 176,429
BT0166
BT0167: BT0167 - conserved hypothetical protein (NCBI ptt file), at 176,559 to 176,897
BT0167
BT0168: BT0168 - conserved hypothetical protein (NCBI ptt file), at 176,890 to 177,189
BT0168
BT0169: BT0169 - conserved hypothetical protein (NCBI ptt file), at 177,407 to 178,462
BT0169
BT0170: BT0170 - conserved hypothetical protein (NCBI ptt file), at 178,582 to 178,935
BT0170
BT0171: BT0171 - conserved hypothetical protein (NCBI ptt file), at 179,107 to 180,390
BT0171
Position (kb) 177
178
179 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 176.497 kb on - strand at 176.571 kb on + strand at 176.591 kb on + strand at 176.609 kb on + strand, within BT0167 at 176.635 kb on - strand, within BT0167 at 176.640 kb on + strand, within BT0167 at 176.640 kb on + strand, within BT0167 at 176.646 kb on - strand, within BT0167 at 176.651 kb on - strand, within BT0167 at 176.664 kb on - strand, within BT0167 at 176.665 kb on + strand, within BT0167 at 176.702 kb on + strand, within BT0167 at 176.703 kb on - strand, within BT0167 at 176.703 kb on - strand, within BT0167 at 176.732 kb on + strand, within BT0167 at 176.732 kb on + strand, within BT0167 at 176.733 kb on - strand, within BT0167 at 176.738 kb on + strand, within BT0167 at 176.757 kb on + strand, within BT0167 at 176.792 kb on + strand, within BT0167 at 176.793 kb on - strand, within BT0167 at 176.809 kb on + strand, within BT0167 at 176.883 kb on + strand at 177.154 kb on + strand, within BT0168 at 177.156 kb on + strand, within BT0168 at 177.217 kb on - strand at 177.217 kb on - strand at 177.289 kb on - strand at 177.365 kb on + strand at 177.365 kb on + strand at 177.366 kb on - strand at 177.366 kb on - strand at 177.369 kb on + strand at 177.370 kb on - strand at 177.370 kb on - strand at 177.401 kb on - strand at 177.427 kb on + strand at 177.488 kb on - strand at 177.509 kb on + strand at 177.543 kb on - strand, within BT0169 at 177.709 kb on + strand, within BT0169 at 177.784 kb on + strand, within BT0169 at 177.793 kb on + strand, within BT0169 at 177.908 kb on - strand, within BT0169 at 178.010 kb on - strand, within BT0169 at 178.010 kb on - strand, within BT0169 at 178.025 kb on - strand, within BT0169 at 178.027 kb on - strand, within BT0169 at 178.348 kb on + strand, within BT0169 at 178.349 kb on - strand, within BT0169 at 178.440 kb on - strand at 178.448 kb on + strand at 178.475 kb on - strand at 178.513 kb on + strand at 178.515 kb on + strand at 178.523 kb on - strand at 178.535 kb on + strand at 178.621 kb on + strand, within BT0170 at 178.675 kb on + strand, within BT0170 at 178.706 kb on - strand, within BT0170 at 178.718 kb on - strand, within BT0170 at 178.873 kb on + strand, within BT0170 at 178.912 kb on + strand at 178.931 kb on - strand at 178.957 kb on - strand at 178.957 kb on - strand at 178.962 kb on - strand at 178.962 kb on - strand at 178.992 kb on + strand at 179.002 kb on + strand at 179.078 kb on + strand at 179.104 kb on - strand at 179.119 kb on - strand at 179.162 kb on - strand at 179.198 kb on + strand at 179.307 kb on + strand, within BT0171 at 179.336 kb on + strand, within BT0171 at 179.337 kb on - strand, within BT0171 at 179.341 kb on - strand, within BT0171 at 179.391 kb on - strand, within BT0171 at 179.424 kb on + strand, within BT0171
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Ramoplanin 0.003125 mM remove 176,497 - -0.0 176,571 + -1.0 176,591 + -0.6 176,609 + BT0167 0.15 -0.6 176,635 - BT0167 0.22 -1.6 176,640 + BT0167 0.24 -2.4 176,640 + BT0167 0.24 -0.6 176,646 - BT0167 0.26 -0.6 176,651 - BT0167 0.27 -0.3 176,664 - BT0167 0.31 +1.4 176,665 + BT0167 0.31 -0.5 176,702 + BT0167 0.42 +0.4 176,703 - BT0167 0.42 +0.3 176,703 - BT0167 0.42 +0.2 176,732 + BT0167 0.51 -1.1 176,732 + BT0167 0.51 +0.9 176,733 - BT0167 0.51 -0.8 176,738 + BT0167 0.53 -1.1 176,757 + BT0167 0.58 +0.4 176,792 + BT0167 0.69 -1.9 176,793 - BT0167 0.69 +0.4 176,809 + BT0167 0.74 -0.6 176,883 + -0.6 177,154 + BT0168 0.88 -0.6 177,156 + BT0168 0.89 -0.6 177,217 - -2.6 177,217 - +0.4 177,289 - -1.6 177,365 + +0.4 177,365 + -2.1 177,366 - -1.1 177,366 - -0.4 177,369 + -1.1 177,370 - -1.1 177,370 - -1.2 177,401 - -2.4 177,427 + -0.6 177,488 - +0.0 177,509 + -2.6 177,543 - BT0169 0.13 +0.4 177,709 + BT0169 0.29 +0.5 177,784 + BT0169 0.36 +0.4 177,793 + BT0169 0.37 -0.1 177,908 - BT0169 0.47 +0.1 178,010 - BT0169 0.57 +0.3 178,010 - BT0169 0.57 -1.9 178,025 - BT0169 0.59 -1.6 178,027 - BT0169 0.59 -0.6 178,348 + BT0169 0.89 +0.5 178,349 - BT0169 0.89 +0.5 178,440 - +0.5 178,448 + -1.0 178,475 - -3.1 178,513 + +2.0 178,515 + -1.3 178,523 - -0.6 178,535 + -1.1 178,621 + BT0170 0.11 -3.0 178,675 + BT0170 0.26 -3.0 178,706 - BT0170 0.35 +1.5 178,718 - BT0170 0.38 -1.1 178,873 + BT0170 0.82 -1.1 178,912 + -0.5 178,931 - -0.3 178,957 - -0.8 178,957 - -2.7 178,962 - +0.5 178,962 - +1.5 178,992 + -0.8 179,002 + +1.5 179,078 + -1.3 179,104 - -0.5 179,119 - -0.5 179,162 - -1.5 179,198 + -1.1 179,307 + BT0171 0.16 -0.5 179,336 + BT0171 0.18 +0.5 179,337 - BT0171 0.18 +0.5 179,341 - BT0171 0.18 -1.1 179,391 - BT0171 0.22 -0.8 179,424 + BT0171 0.25 -1.1
Or see this region's nucleotide sequence