Experiment: KB with Conessine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3818 and Psyr_3819 are separated by 56 nucleotides Psyr_3819 and Psyr_3820 are separated by 443 nucleotides
Psyr_3818: Psyr_3818 - conserved hypothetical protein, at 4,543,818 to 4,544,201
_3818
Psyr_3819: Psyr_3819 - Protein of unknown function DUF1349, at 4,544,258 to 4,544,833
_3819
Psyr_3820: Psyr_3820 - conserved domain protein, at 4,545,277 to 4,545,822
_3820
Position (kb)
4544
4545 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4543.335 kb on - strand at 4543.452 kb on + strand at 4543.453 kb on - strand at 4543.506 kb on + strand at 4543.507 kb on - strand at 4543.528 kb on + strand at 4543.537 kb on + strand at 4543.538 kb on - strand at 4543.545 kb on - strand at 4543.545 kb on - strand at 4543.604 kb on - strand at 4543.606 kb on + strand at 4543.643 kb on + strand at 4543.643 kb on + strand at 4543.643 kb on + strand at 4543.644 kb on - strand at 4543.644 kb on - strand at 4543.647 kb on + strand at 4543.648 kb on - strand at 4543.648 kb on - strand at 4543.653 kb on + strand at 4543.665 kb on + strand at 4543.719 kb on + strand at 4543.720 kb on - strand at 4543.725 kb on + strand at 4543.727 kb on + strand at 4543.727 kb on + strand at 4543.728 kb on - strand at 4543.728 kb on - strand at 4543.730 kb on - strand at 4543.730 kb on - strand at 4543.730 kb on - strand at 4543.730 kb on - strand at 4543.730 kb on - strand at 4543.743 kb on + strand at 4543.760 kb on + strand at 4543.796 kb on + strand at 4543.796 kb on + strand at 4543.796 kb on + strand at 4543.796 kb on + strand at 4543.796 kb on + strand at 4543.796 kb on + strand at 4543.796 kb on + strand at 4543.796 kb on + strand at 4543.797 kb on - strand at 4544.025 kb on + strand, within Psyr_3818 at 4544.025 kb on + strand, within Psyr_3818 at 4544.025 kb on + strand, within Psyr_3818 at 4544.025 kb on + strand, within Psyr_3818 at 4544.025 kb on + strand, within Psyr_3818 at 4544.026 kb on - strand, within Psyr_3818 at 4544.026 kb on - strand, within Psyr_3818 at 4544.026 kb on - strand, within Psyr_3818 at 4544.090 kb on + strand, within Psyr_3818 at 4544.090 kb on + strand, within Psyr_3818 at 4544.092 kb on + strand, within Psyr_3818 at 4544.093 kb on - strand, within Psyr_3818 at 4544.146 kb on - strand, within Psyr_3818 at 4544.146 kb on - strand, within Psyr_3818 at 4544.209 kb on + strand at 4544.210 kb on - strand at 4544.254 kb on + strand at 4544.255 kb on - strand at 4544.255 kb on - strand at 4544.255 kb on - strand at 4544.255 kb on - strand at 4544.360 kb on + strand, within Psyr_3819 at 4544.360 kb on + strand, within Psyr_3819 at 4544.360 kb on + strand, within Psyr_3819 at 4544.361 kb on - strand, within Psyr_3819 at 4544.366 kb on - strand, within Psyr_3819 at 4544.366 kb on - strand, within Psyr_3819 at 4544.367 kb on + strand, within Psyr_3819 at 4544.367 kb on + strand, within Psyr_3819 at 4544.368 kb on - strand, within Psyr_3819 at 4544.445 kb on - strand, within Psyr_3819 at 4544.498 kb on + strand, within Psyr_3819 at 4544.499 kb on - strand, within Psyr_3819 at 4544.499 kb on - strand, within Psyr_3819 at 4544.499 kb on - strand, within Psyr_3819 at 4544.499 kb on - strand, within Psyr_3819 at 4544.629 kb on - strand, within Psyr_3819 at 4544.725 kb on - strand, within Psyr_3819 at 4544.877 kb on + strand at 4544.877 kb on + strand at 4544.877 kb on + strand at 4544.877 kb on + strand at 4544.877 kb on + strand at 4544.878 kb on - strand at 4544.878 kb on - strand at 4544.878 kb on - strand at 4544.878 kb on - strand at 4544.878 kb on - strand at 4544.878 kb on - strand at 4544.885 kb on - strand at 4544.894 kb on + strand at 4544.894 kb on + strand at 4544.895 kb on - strand at 4544.908 kb on + strand at 4544.909 kb on - strand at 4544.962 kb on + strand at 4544.990 kb on - strand at 4545.092 kb on - strand at 4545.129 kb on + strand at 4545.140 kb on + strand at 4545.140 kb on + strand at 4545.140 kb on + strand at 4545.140 kb on + strand at 4545.141 kb on - strand at 4545.147 kb on + strand at 4545.147 kb on + strand at 4545.148 kb on - strand at 4545.553 kb on + strand, within Psyr_3820 at 4545.553 kb on + strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Conessine 10 mM remove 4,543,335 - +0.4 4,543,452 + +3.2 4,543,453 - -0.5 4,543,506 + +0.1 4,543,507 - -1.7 4,543,528 + +0.4 4,543,537 + +0.8 4,543,538 - +0.2 4,543,545 - +0.6 4,543,545 - -0.0 4,543,604 - +0.3 4,543,606 + +1.2 4,543,643 + +0.2 4,543,643 + -1.0 4,543,643 + +0.3 4,543,644 - -1.4 4,543,644 - -0.1 4,543,647 + +0.8 4,543,648 - +1.1 4,543,648 - +0.9 4,543,653 + -1.8 4,543,665 + +0.2 4,543,719 + -0.5 4,543,720 - -2.9 4,543,725 + -0.0 4,543,727 + +2.2 4,543,727 + -0.5 4,543,728 - +0.2 4,543,728 - +0.6 4,543,730 - +1.4 4,543,730 - -2.3 4,543,730 - +1.4 4,543,730 - +0.2 4,543,730 - -1.6 4,543,743 + -1.7 4,543,760 + +2.8 4,543,796 + -0.0 4,543,796 + +0.2 4,543,796 + +0.5 4,543,796 + -1.5 4,543,796 + +3.2 4,543,796 + +0.9 4,543,796 + -0.5 4,543,796 + +1.2 4,543,797 - +0.1 4,544,025 + Psyr_3818 0.54 +0.4 4,544,025 + Psyr_3818 0.54 -2.2 4,544,025 + Psyr_3818 0.54 +0.4 4,544,025 + Psyr_3818 0.54 +0.6 4,544,025 + Psyr_3818 0.54 +0.5 4,544,026 - Psyr_3818 0.54 -0.2 4,544,026 - Psyr_3818 0.54 -2.0 4,544,026 - Psyr_3818 0.54 -1.4 4,544,090 + Psyr_3818 0.71 -0.6 4,544,090 + Psyr_3818 0.71 +0.6 4,544,092 + Psyr_3818 0.71 -0.9 4,544,093 - Psyr_3818 0.72 +0.7 4,544,146 - Psyr_3818 0.85 -0.8 4,544,146 - Psyr_3818 0.85 -0.0 4,544,209 + -0.1 4,544,210 - -0.4 4,544,254 + -2.2 4,544,255 - +0.4 4,544,255 - +0.2 4,544,255 - -0.9 4,544,255 - -0.3 4,544,360 + Psyr_3819 0.18 +3.2 4,544,360 + Psyr_3819 0.18 -0.4 4,544,360 + Psyr_3819 0.18 +0.2 4,544,361 - Psyr_3819 0.18 -0.0 4,544,366 - Psyr_3819 0.19 -0.8 4,544,366 - Psyr_3819 0.19 -0.2 4,544,367 + Psyr_3819 0.19 +1.8 4,544,367 + Psyr_3819 0.19 +0.1 4,544,368 - Psyr_3819 0.19 +0.4 4,544,445 - Psyr_3819 0.32 -0.2 4,544,498 + Psyr_3819 0.42 +1.0 4,544,499 - Psyr_3819 0.42 -2.3 4,544,499 - Psyr_3819 0.42 +0.6 4,544,499 - Psyr_3819 0.42 -0.4 4,544,499 - Psyr_3819 0.42 +3.8 4,544,629 - Psyr_3819 0.64 +2.2 4,544,725 - Psyr_3819 0.81 +0.3 4,544,877 + -0.5 4,544,877 + -1.8 4,544,877 + -0.9 4,544,877 + +1.2 4,544,877 + +0.7 4,544,878 - +0.2 4,544,878 - -2.1 4,544,878 - -0.5 4,544,878 - +2.3 4,544,878 - -0.7 4,544,878 - -0.1 4,544,885 - +1.4 4,544,894 + -3.4 4,544,894 + -1.2 4,544,895 - +1.1 4,544,908 + -0.8 4,544,909 - -0.2 4,544,962 + -0.8 4,544,990 - +0.5 4,545,092 - +1.5 4,545,129 + -1.2 4,545,140 + +0.0 4,545,140 + +0.0 4,545,140 + +1.0 4,545,140 + -0.8 4,545,141 - +0.9 4,545,147 + +0.1 4,545,147 + -0.2 4,545,148 - -0.4 4,545,553 + Psyr_3820 0.51 +0.8 4,545,553 + Psyr_3820 0.51 +1.7 4,545,554 - Psyr_3820 0.51 +1.2 4,545,554 - Psyr_3820 0.51 -0.9 4,545,554 - Psyr_3820 0.51 -0.2 4,545,554 - Psyr_3820 0.51 +0.3 4,545,554 - Psyr_3820 0.51 -2.5 4,545,554 - Psyr_3820 0.51 -1.7
Or see this region's nucleotide sequence