Experiment: KB with Conessine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2852 and Psyr_2853 are separated by 147 nucleotides Psyr_2853 and Psyr_2854 are separated by 311 nucleotides Psyr_2854 and Psyr_2855 are separated by 23 nucleotides
Psyr_2852: Psyr_2852 - conserved hypothetical protein, at 3,424,261 to 3,424,794
_2852
Psyr_2853: Psyr_2853 - CDS, at 3,424,942 to 3,425,439
_2853
Psyr_2854: Psyr_2854 - conserved hypothetical protein, at 3,425,751 to 3,426,734
_2854
Psyr_2855: Psyr_2855 - methionine synthase (B12-independent), at 3,426,758 to 3,427,789
_2855
Position (kb)
3425
3426
3427 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3424.774 kb on + strand at 3424.774 kb on + strand at 3424.860 kb on + strand at 3424.860 kb on + strand at 3424.861 kb on - strand at 3424.861 kb on - strand at 3424.861 kb on - strand at 3424.916 kb on - strand at 3424.916 kb on - strand at 3424.943 kb on + strand at 3424.943 kb on + strand at 3424.944 kb on - strand at 3425.058 kb on + strand, within Psyr_2853 at 3425.058 kb on + strand, within Psyr_2853 at 3425.062 kb on + strand, within Psyr_2853 at 3425.062 kb on + strand, within Psyr_2853 at 3425.062 kb on + strand, within Psyr_2853 at 3425.063 kb on - strand, within Psyr_2853 at 3425.063 kb on - strand, within Psyr_2853 at 3425.063 kb on - strand, within Psyr_2853 at 3425.120 kb on - strand, within Psyr_2853 at 3425.133 kb on + strand, within Psyr_2853 at 3425.134 kb on - strand, within Psyr_2853 at 3425.134 kb on - strand, within Psyr_2853 at 3425.171 kb on + strand, within Psyr_2853 at 3425.171 kb on + strand, within Psyr_2853 at 3425.171 kb on + strand, within Psyr_2853 at 3425.171 kb on + strand, within Psyr_2853 at 3425.172 kb on - strand, within Psyr_2853 at 3425.172 kb on - strand, within Psyr_2853 at 3425.176 kb on - strand, within Psyr_2853 at 3425.176 kb on - strand, within Psyr_2853 at 3425.196 kb on + strand, within Psyr_2853 at 3425.197 kb on - strand, within Psyr_2853 at 3425.197 kb on - strand, within Psyr_2853 at 3425.271 kb on - strand, within Psyr_2853 at 3425.341 kb on + strand, within Psyr_2853 at 3425.341 kb on + strand, within Psyr_2853 at 3425.342 kb on - strand, within Psyr_2853 at 3425.407 kb on + strand at 3425.407 kb on + strand at 3425.407 kb on + strand at 3425.407 kb on + strand at 3425.408 kb on - strand at 3425.408 kb on - strand at 3425.456 kb on + strand at 3425.456 kb on + strand at 3425.457 kb on - strand at 3425.457 kb on - strand at 3425.458 kb on + strand at 3425.510 kb on - strand at 3425.551 kb on + strand at 3425.551 kb on + strand at 3425.552 kb on - strand at 3425.608 kb on + strand at 3425.608 kb on + strand at 3425.639 kb on + strand at 3425.693 kb on + strand at 3425.802 kb on + strand at 3425.883 kb on - strand, within Psyr_2854 at 3425.885 kb on + strand, within Psyr_2854 at 3425.954 kb on + strand, within Psyr_2854 at 3425.996 kb on + strand, within Psyr_2854 at 3426.109 kb on - strand, within Psyr_2854 at 3426.238 kb on - strand, within Psyr_2854 at 3426.297 kb on + strand, within Psyr_2854 at 3426.297 kb on + strand, within Psyr_2854 at 3426.298 kb on - strand, within Psyr_2854 at 3426.328 kb on + strand, within Psyr_2854 at 3426.373 kb on - strand, within Psyr_2854 at 3426.403 kb on - strand, within Psyr_2854 at 3426.468 kb on + strand, within Psyr_2854 at 3426.469 kb on - strand, within Psyr_2854 at 3426.486 kb on - strand, within Psyr_2854 at 3426.540 kb on + strand, within Psyr_2854 at 3426.540 kb on + strand, within Psyr_2854 at 3426.540 kb on + strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.541 kb on - strand, within Psyr_2854 at 3426.618 kb on + strand, within Psyr_2854 at 3426.619 kb on - strand, within Psyr_2854 at 3426.690 kb on + strand at 3426.690 kb on + strand at 3426.740 kb on + strand at 3426.751 kb on + strand at 3426.751 kb on + strand at 3426.757 kb on + strand at 3426.970 kb on + strand, within Psyr_2855 at 3426.970 kb on + strand, within Psyr_2855 at 3426.976 kb on + strand, within Psyr_2855 at 3426.976 kb on + strand, within Psyr_2855 at 3427.021 kb on + strand, within Psyr_2855 at 3427.194 kb on - strand, within Psyr_2855 at 3427.369 kb on + strand at 3427.390 kb on + strand, within Psyr_2855 at 3427.390 kb on + strand, within Psyr_2855 at 3427.390 kb on + strand, within Psyr_2855 at 3427.391 kb on - strand, within Psyr_2855 at 3427.391 kb on - strand, within Psyr_2855 at 3427.461 kb on - strand, within Psyr_2855
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Conessine 10 mM remove 3,424,774 + -0.9 3,424,774 + +2.1 3,424,860 + +1.2 3,424,860 + +0.0 3,424,861 - -2.2 3,424,861 - +0.6 3,424,861 - -0.6 3,424,916 - -0.3 3,424,916 - -0.5 3,424,943 + -3.9 3,424,943 + -0.7 3,424,944 - -0.9 3,425,058 + Psyr_2853 0.23 -0.9 3,425,058 + Psyr_2853 0.23 +0.1 3,425,062 + Psyr_2853 0.24 +1.0 3,425,062 + Psyr_2853 0.24 -1.4 3,425,062 + Psyr_2853 0.24 -2.3 3,425,063 - Psyr_2853 0.24 -0.8 3,425,063 - Psyr_2853 0.24 -0.2 3,425,063 - Psyr_2853 0.24 -0.7 3,425,120 - Psyr_2853 0.36 -1.9 3,425,133 + Psyr_2853 0.38 -2.2 3,425,134 - Psyr_2853 0.39 +0.8 3,425,134 - Psyr_2853 0.39 -0.2 3,425,171 + Psyr_2853 0.46 -1.9 3,425,171 + Psyr_2853 0.46 -1.2 3,425,171 + Psyr_2853 0.46 -2.1 3,425,171 + Psyr_2853 0.46 +0.2 3,425,172 - Psyr_2853 0.46 +0.5 3,425,172 - Psyr_2853 0.46 -1.4 3,425,176 - Psyr_2853 0.47 -0.2 3,425,176 - Psyr_2853 0.47 -1.2 3,425,196 + Psyr_2853 0.51 +0.1 3,425,197 - Psyr_2853 0.51 -0.9 3,425,197 - Psyr_2853 0.51 -1.6 3,425,271 - Psyr_2853 0.66 +0.9 3,425,341 + Psyr_2853 0.80 -0.7 3,425,341 + Psyr_2853 0.80 -0.8 3,425,342 - Psyr_2853 0.80 +0.1 3,425,407 + -3.7 3,425,407 + -2.5 3,425,407 + +3.2 3,425,407 + -1.8 3,425,408 - -0.6 3,425,408 - +2.5 3,425,456 + +0.8 3,425,456 + +0.4 3,425,457 - +0.7 3,425,457 - +0.5 3,425,458 + +3.8 3,425,510 - -1.1 3,425,551 + -1.6 3,425,551 + +0.5 3,425,552 - -0.4 3,425,608 + +1.9 3,425,608 + +1.2 3,425,639 + +1.2 3,425,693 + +0.2 3,425,802 + -0.9 3,425,883 - Psyr_2854 0.13 -1.9 3,425,885 + Psyr_2854 0.14 -1.1 3,425,954 + Psyr_2854 0.21 -1.3 3,425,996 + Psyr_2854 0.25 -0.6 3,426,109 - Psyr_2854 0.36 -0.4 3,426,238 - Psyr_2854 0.49 -0.4 3,426,297 + Psyr_2854 0.55 -1.4 3,426,297 + Psyr_2854 0.55 +1.8 3,426,298 - Psyr_2854 0.56 -2.2 3,426,328 + Psyr_2854 0.59 -1.8 3,426,373 - Psyr_2854 0.63 -0.9 3,426,403 - Psyr_2854 0.66 -0.2 3,426,468 + Psyr_2854 0.73 -1.8 3,426,469 - Psyr_2854 0.73 -1.9 3,426,486 - Psyr_2854 0.75 -2.5 3,426,540 + Psyr_2854 0.80 -1.6 3,426,540 + Psyr_2854 0.80 -0.7 3,426,540 + Psyr_2854 0.80 +2.2 3,426,541 - Psyr_2854 0.80 -2.5 3,426,541 - Psyr_2854 0.80 -2.1 3,426,541 - Psyr_2854 0.80 +2.2 3,426,541 - Psyr_2854 0.80 -2.8 3,426,618 + Psyr_2854 0.88 -0.4 3,426,619 - Psyr_2854 0.88 -2.7 3,426,690 + -0.5 3,426,690 + +0.0 3,426,740 + +1.0 3,426,751 + -0.2 3,426,751 + +0.2 3,426,757 + +0.5 3,426,970 + Psyr_2855 0.21 +0.5 3,426,970 + Psyr_2855 0.21 +1.2 3,426,976 + Psyr_2855 0.21 -1.6 3,426,976 + Psyr_2855 0.21 +0.9 3,427,021 + Psyr_2855 0.25 +0.5 3,427,194 - Psyr_2855 0.42 -1.0 3,427,369 + +3.5 3,427,390 + Psyr_2855 0.61 +0.7 3,427,390 + Psyr_2855 0.61 -0.4 3,427,390 + Psyr_2855 0.61 -1.6 3,427,391 - Psyr_2855 0.61 -1.2 3,427,391 - Psyr_2855 0.61 +1.2 3,427,461 - Psyr_2855 0.68 -1.4
Or see this region's nucleotide sequence