Experiment: KB with Conessine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2849 and Psyr_2850 are separated by 82 nucleotides Psyr_2850 and Psyr_2851 are separated by 81 nucleotides Psyr_2851 and Psyr_2852 are separated by 298 nucleotides Psyr_2852 and Psyr_2853 are separated by 147 nucleotides
Psyr_2849: Psyr_2849 - CDS, at 3,422,438 to 3,423,085
_2849
Psyr_2850: Psyr_2850 - hypothetical protein, at 3,423,168 to 3,423,527
_2850
Psyr_2851: Psyr_2851 - conserved hypothetical protein, at 3,423,609 to 3,423,962
_2851
Psyr_2852: Psyr_2852 - conserved hypothetical protein, at 3,424,261 to 3,424,794
_2852
Psyr_2853: Psyr_2853 - CDS, at 3,424,942 to 3,425,439
_2853
Position (kb)
3423
3424 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3423.114 kb on + strand at 3423.146 kb on + strand at 3423.146 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.189 kb on - strand at 3423.189 kb on - strand at 3423.236 kb on - strand, within Psyr_2850 at 3423.337 kb on - strand, within Psyr_2850 at 3423.337 kb on - strand, within Psyr_2850 at 3423.337 kb on - strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.361 kb on - strand, within Psyr_2850 at 3423.361 kb on - strand, within Psyr_2850 at 3423.492 kb on + strand at 3423.594 kb on + strand at 3423.607 kb on + strand at 3423.826 kb on - strand, within Psyr_2851 at 3423.845 kb on - strand, within Psyr_2851 at 3423.873 kb on + strand, within Psyr_2851 at 3423.874 kb on - strand, within Psyr_2851 at 3423.993 kb on + strand at 3423.993 kb on + strand at 3424.083 kb on - strand at 3424.083 kb on - strand at 3424.083 kb on - strand at 3424.129 kb on + strand at 3424.130 kb on - strand at 3424.130 kb on - strand at 3424.130 kb on - strand at 3424.130 kb on - strand at 3424.130 kb on - strand at 3424.155 kb on + strand at 3424.156 kb on - strand at 3424.156 kb on - strand at 3424.192 kb on + strand at 3424.193 kb on - strand at 3424.193 kb on - strand at 3424.253 kb on + strand at 3424.268 kb on + strand at 3424.268 kb on + strand at 3424.268 kb on + strand at 3424.269 kb on - strand at 3424.269 kb on - strand at 3424.269 kb on - strand at 3424.276 kb on - strand at 3424.396 kb on + strand at 3424.397 kb on - strand, within Psyr_2852 at 3424.397 kb on - strand, within Psyr_2852 at 3424.397 kb on - strand, within Psyr_2852 at 3424.477 kb on + strand, within Psyr_2852 at 3424.477 kb on + strand, within Psyr_2852 at 3424.478 kb on - strand, within Psyr_2852 at 3424.478 kb on - strand, within Psyr_2852 at 3424.478 kb on - strand, within Psyr_2852 at 3424.478 kb on - strand, within Psyr_2852 at 3424.533 kb on + strand, within Psyr_2852 at 3424.559 kb on + strand, within Psyr_2852 at 3424.613 kb on - strand, within Psyr_2852 at 3424.774 kb on + strand at 3424.774 kb on + strand at 3424.860 kb on + strand at 3424.860 kb on + strand at 3424.861 kb on - strand at 3424.861 kb on - strand at 3424.861 kb on - strand at 3424.916 kb on - strand at 3424.916 kb on - strand at 3424.943 kb on + strand at 3424.943 kb on + strand at 3424.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Conessine 10 mM remove 3,423,114 + +0.6 3,423,146 + +2.2 3,423,146 + -0.8 3,423,184 + +0.5 3,423,184 + +1.2 3,423,184 + +1.2 3,423,184 + -1.8 3,423,184 + -0.6 3,423,184 + -1.4 3,423,184 + -1.8 3,423,185 - -0.1 3,423,185 - -2.6 3,423,185 - +0.6 3,423,185 - +2.5 3,423,185 - +0.1 3,423,185 - -1.3 3,423,189 - +0.3 3,423,189 - +1.1 3,423,236 - Psyr_2850 0.19 -0.4 3,423,337 - Psyr_2850 0.47 -1.7 3,423,337 - Psyr_2850 0.47 +1.7 3,423,337 - Psyr_2850 0.47 -0.4 3,423,360 + Psyr_2850 0.53 -0.8 3,423,360 + Psyr_2850 0.53 +0.7 3,423,360 + Psyr_2850 0.53 -0.3 3,423,360 + Psyr_2850 0.53 -0.6 3,423,361 - Psyr_2850 0.54 -0.2 3,423,361 - Psyr_2850 0.54 -0.8 3,423,492 + +2.2 3,423,594 + +0.9 3,423,607 + -0.1 3,423,826 - Psyr_2851 0.61 -1.1 3,423,845 - Psyr_2851 0.67 +0.7 3,423,873 + Psyr_2851 0.75 +0.5 3,423,874 - Psyr_2851 0.75 -0.1 3,423,993 + +0.6 3,423,993 + -2.2 3,424,083 - -0.6 3,424,083 - -1.4 3,424,083 - +2.1 3,424,129 + -0.4 3,424,130 - +0.3 3,424,130 - +0.2 3,424,130 - -0.4 3,424,130 - -1.4 3,424,130 - +3.5 3,424,155 + +0.2 3,424,156 - -0.4 3,424,156 - -1.6 3,424,192 + +1.1 3,424,193 - -1.6 3,424,193 - +1.3 3,424,253 + -0.0 3,424,268 + -1.4 3,424,268 + -0.4 3,424,268 + -1.0 3,424,269 - -1.4 3,424,269 - -1.5 3,424,269 - +1.6 3,424,276 - -2.2 3,424,396 + -1.9 3,424,397 - Psyr_2852 0.25 -1.6 3,424,397 - Psyr_2852 0.25 +0.6 3,424,397 - Psyr_2852 0.25 -0.9 3,424,477 + Psyr_2852 0.40 -0.1 3,424,477 + Psyr_2852 0.40 -0.2 3,424,478 - Psyr_2852 0.41 +0.5 3,424,478 - Psyr_2852 0.41 -0.4 3,424,478 - Psyr_2852 0.41 -0.7 3,424,478 - Psyr_2852 0.41 -0.5 3,424,533 + Psyr_2852 0.51 -0.7 3,424,559 + Psyr_2852 0.56 +0.2 3,424,613 - Psyr_2852 0.66 -1.7 3,424,774 + -0.9 3,424,774 + +2.1 3,424,860 + +1.2 3,424,860 + +0.0 3,424,861 - -2.2 3,424,861 - +0.6 3,424,861 - -0.6 3,424,916 - -0.3 3,424,916 - -0.5 3,424,943 + -3.9 3,424,943 + -0.7 3,424,944 - -0.9
Or see this region's nucleotide sequence