Experiment: KB with Conessine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0402 and Psyr_0403 are separated by 206 nucleotides Psyr_0403 and Psyr_0404 overlap by 1 nucleotides Psyr_0404 and Psyr_0405 overlap by 4 nucleotides
Psyr_0402: Psyr_0402 - Peptidoglycan glycosyltransferase, at 430,973 to 433,417
_0402
Psyr_0403: Psyr_0403 - Type IV pilus assembly protein PilM, at 433,624 to 434,688
_0403
Psyr_0404: Psyr_0404 - Fimbrial assembly, at 434,688 to 435,263
_0404
Psyr_0405: Psyr_0405 - Pilus assembly protein, PilO, at 435,260 to 435,883
_0405
Position (kb)
433
434
435 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 432.627 kb on + strand, within Psyr_0402 at 432.627 kb on + strand, within Psyr_0402 at 432.627 kb on + strand, within Psyr_0402 at 432.628 kb on - strand, within Psyr_0402 at 432.660 kb on + strand, within Psyr_0402 at 432.660 kb on + strand, within Psyr_0402 at 432.660 kb on + strand, within Psyr_0402 at 432.660 kb on + strand, within Psyr_0402 at 432.661 kb on - strand, within Psyr_0402 at 432.726 kb on + strand, within Psyr_0402 at 432.726 kb on + strand, within Psyr_0402 at 432.726 kb on + strand, within Psyr_0402 at 432.726 kb on + strand, within Psyr_0402 at 432.726 kb on + strand, within Psyr_0402 at 432.726 kb on + strand, within Psyr_0402 at 432.727 kb on - strand, within Psyr_0402 at 432.727 kb on - strand, within Psyr_0402 at 432.727 kb on - strand, within Psyr_0402 at 432.727 kb on - strand, within Psyr_0402 at 432.727 kb on - strand, within Psyr_0402 at 432.842 kb on + strand, within Psyr_0402 at 432.842 kb on + strand, within Psyr_0402 at 432.842 kb on + strand, within Psyr_0402 at 432.842 kb on + strand, within Psyr_0402 at 432.842 kb on + strand, within Psyr_0402 at 432.842 kb on + strand, within Psyr_0402 at 432.842 kb on + strand, within Psyr_0402 at 432.843 kb on - strand, within Psyr_0402 at 432.843 kb on - strand, within Psyr_0402 at 432.843 kb on - strand, within Psyr_0402 at 432.864 kb on + strand, within Psyr_0402 at 432.864 kb on + strand, within Psyr_0402 at 432.864 kb on + strand, within Psyr_0402 at 432.864 kb on + strand, within Psyr_0402 at 432.865 kb on - strand, within Psyr_0402 at 432.865 kb on - strand, within Psyr_0402 at 432.867 kb on + strand, within Psyr_0402 at 432.867 kb on + strand, within Psyr_0402 at 432.867 kb on + strand, within Psyr_0402 at 432.867 kb on + strand, within Psyr_0402 at 432.868 kb on - strand, within Psyr_0402 at 432.868 kb on - strand, within Psyr_0402 at 432.868 kb on - strand, within Psyr_0402 at 432.868 kb on - strand, within Psyr_0402 at 432.868 kb on - strand, within Psyr_0402 at 432.868 kb on - strand, within Psyr_0402 at 432.868 kb on - strand, within Psyr_0402 at 432.868 kb on - strand, within Psyr_0402 at 432.913 kb on - strand, within Psyr_0402 at 432.927 kb on + strand, within Psyr_0402 at 432.928 kb on - strand, within Psyr_0402 at 432.928 kb on - strand, within Psyr_0402 at 432.928 kb on - strand, within Psyr_0402 at 432.952 kb on + strand, within Psyr_0402 at 432.953 kb on - strand, within Psyr_0402 at 432.953 kb on - strand, within Psyr_0402 at 432.953 kb on - strand, within Psyr_0402 at 433.058 kb on - strand, within Psyr_0402 at 433.442 kb on - strand at 433.464 kb on - strand at 433.492 kb on - strand at 433.515 kb on - strand at 433.515 kb on - strand at 433.515 kb on - strand at 433.516 kb on + strand at 433.517 kb on - strand at 433.517 kb on - strand at 433.517 kb on - strand at 433.586 kb on + strand at 433.586 kb on + strand at 433.594 kb on + strand at 433.594 kb on + strand at 433.611 kb on + strand at 433.612 kb on - strand at 433.642 kb on - strand at 433.642 kb on - strand at 433.661 kb on + strand at 433.662 kb on - strand at 433.674 kb on + strand at 433.674 kb on + strand at 433.674 kb on + strand at 433.674 kb on + strand at 433.674 kb on + strand at 433.674 kb on + strand at 433.675 kb on - strand at 433.723 kb on + strand at 433.745 kb on + strand, within Psyr_0403 at 433.746 kb on - strand, within Psyr_0403 at 433.746 kb on - strand, within Psyr_0403 at 433.963 kb on + strand, within Psyr_0403 at 433.963 kb on + strand, within Psyr_0403 at 433.963 kb on + strand, within Psyr_0403 at 433.963 kb on + strand, within Psyr_0403 at 433.963 kb on + strand, within Psyr_0403 at 433.963 kb on + strand, within Psyr_0403 at 433.963 kb on + strand, within Psyr_0403 at 433.963 kb on + strand, within Psyr_0403 at 433.963 kb on + strand, within Psyr_0403 at 433.963 kb on + strand, within Psyr_0403 at 433.964 kb on - strand, within Psyr_0403 at 433.964 kb on - strand, within Psyr_0403 at 433.964 kb on - strand, within Psyr_0403 at 433.964 kb on - strand, within Psyr_0403 at 433.964 kb on - strand, within Psyr_0403 at 433.964 kb on - strand, within Psyr_0403 at 434.272 kb on + strand, within Psyr_0403 at 434.272 kb on + strand, within Psyr_0403 at 434.272 kb on + strand, within Psyr_0403 at 434.272 kb on + strand, within Psyr_0403 at 434.272 kb on + strand, within Psyr_0403 at 434.272 kb on + strand, within Psyr_0403 at 434.272 kb on + strand, within Psyr_0403 at 434.273 kb on - strand, within Psyr_0403 at 434.273 kb on - strand, within Psyr_0403 at 434.273 kb on - strand, within Psyr_0403 at 434.389 kb on + strand, within Psyr_0403 at 434.389 kb on + strand, within Psyr_0403 at 434.389 kb on + strand, within Psyr_0403 at 434.390 kb on - strand, within Psyr_0403 at 434.390 kb on - strand, within Psyr_0403 at 434.390 kb on - strand, within Psyr_0403 at 434.390 kb on - strand, within Psyr_0403 at 434.476 kb on + strand, within Psyr_0403 at 434.476 kb on + strand, within Psyr_0403 at 434.477 kb on - strand, within Psyr_0403 at 434.477 kb on - strand, within Psyr_0403 at 434.477 kb on - strand, within Psyr_0403 at 434.704 kb on + strand at 434.704 kb on + strand at 434.704 kb on + strand at 434.704 kb on + strand at 434.704 kb on + strand at 434.704 kb on + strand at 434.705 kb on - strand at 434.705 kb on - strand at 434.705 kb on - strand at 434.705 kb on - strand at 434.705 kb on - strand at 434.705 kb on - strand at 434.811 kb on + strand, within Psyr_0404 at 434.812 kb on - strand, within Psyr_0404 at 435.027 kb on + strand, within Psyr_0404 at 435.028 kb on - strand, within Psyr_0404 at 435.028 kb on - strand, within Psyr_0404 at 435.028 kb on - strand, within Psyr_0404 at 435.030 kb on + strand, within Psyr_0404 at 435.214 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.398 kb on + strand, within Psyr_0405 at 435.398 kb on + strand, within Psyr_0405 at 435.398 kb on + strand, within Psyr_0405 at 435.399 kb on - strand, within Psyr_0405 at 435.512 kb on + strand, within Psyr_0405 at 435.513 kb on - strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Conessine 10 mM remove 432,627 + Psyr_0402 0.68 +0.8 432,627 + Psyr_0402 0.68 -2.3 432,627 + Psyr_0402 0.68 -1.5 432,628 - Psyr_0402 0.68 +0.4 432,660 + Psyr_0402 0.69 +0.4 432,660 + Psyr_0402 0.69 -1.5 432,660 + Psyr_0402 0.69 -0.5 432,660 + Psyr_0402 0.69 -1.6 432,661 - Psyr_0402 0.69 -0.8 432,726 + Psyr_0402 0.72 +1.2 432,726 + Psyr_0402 0.72 +0.0 432,726 + Psyr_0402 0.72 -1.2 432,726 + Psyr_0402 0.72 -1.4 432,726 + Psyr_0402 0.72 -1.2 432,726 + Psyr_0402 0.72 -0.9 432,727 - Psyr_0402 0.72 -0.0 432,727 - Psyr_0402 0.72 +0.1 432,727 - Psyr_0402 0.72 +0.6 432,727 - Psyr_0402 0.72 -1.5 432,727 - Psyr_0402 0.72 +1.0 432,842 + Psyr_0402 0.76 -0.6 432,842 + Psyr_0402 0.76 -0.4 432,842 + Psyr_0402 0.76 -0.7 432,842 + Psyr_0402 0.76 -0.7 432,842 + Psyr_0402 0.76 -0.9 432,842 + Psyr_0402 0.76 +0.6 432,842 + Psyr_0402 0.76 -1.1 432,843 - Psyr_0402 0.76 -0.7 432,843 - Psyr_0402 0.76 -1.4 432,843 - Psyr_0402 0.76 -0.2 432,864 + Psyr_0402 0.77 -0.3 432,864 + Psyr_0402 0.77 -1.0 432,864 + Psyr_0402 0.77 +0.0 432,864 + Psyr_0402 0.77 -2.1 432,865 - Psyr_0402 0.77 -1.5 432,865 - Psyr_0402 0.77 +2.0 432,867 + Psyr_0402 0.77 +1.4 432,867 + Psyr_0402 0.77 +1.0 432,867 + Psyr_0402 0.77 -0.7 432,867 + Psyr_0402 0.77 -2.9 432,868 - Psyr_0402 0.78 +0.9 432,868 - Psyr_0402 0.78 -0.0 432,868 - Psyr_0402 0.78 +0.3 432,868 - Psyr_0402 0.78 -3.3 432,868 - Psyr_0402 0.78 -0.9 432,868 - Psyr_0402 0.78 +1.7 432,868 - Psyr_0402 0.78 -2.5 432,868 - Psyr_0402 0.78 -0.8 432,913 - Psyr_0402 0.79 -2.1 432,927 + Psyr_0402 0.80 -0.9 432,928 - Psyr_0402 0.80 -1.0 432,928 - Psyr_0402 0.80 -0.7 432,928 - Psyr_0402 0.80 +0.2 432,952 + Psyr_0402 0.81 -0.8 432,953 - Psyr_0402 0.81 +0.7 432,953 - Psyr_0402 0.81 -1.6 432,953 - Psyr_0402 0.81 -2.6 433,058 - Psyr_0402 0.85 -0.8 433,442 - +0.8 433,464 - +4.0 433,492 - +1.8 433,515 - -0.2 433,515 - +1.4 433,515 - -0.5 433,516 + -1.4 433,517 - +0.5 433,517 - +0.6 433,517 - +0.5 433,586 + -0.7 433,586 + +0.7 433,594 + -0.6 433,594 + -2.1 433,611 + -0.9 433,612 - +0.9 433,642 - -1.7 433,642 - +0.5 433,661 + +1.3 433,662 - -0.6 433,674 + -0.3 433,674 + -2.4 433,674 + +0.5 433,674 + +0.6 433,674 + +1.3 433,674 + -0.0 433,675 - +0.4 433,723 + -1.3 433,745 + Psyr_0403 0.11 +0.2 433,746 - Psyr_0403 0.11 +0.8 433,746 - Psyr_0403 0.11 -0.0 433,963 + Psyr_0403 0.32 -0.4 433,963 + Psyr_0403 0.32 -0.7 433,963 + Psyr_0403 0.32 -1.2 433,963 + Psyr_0403 0.32 -0.7 433,963 + Psyr_0403 0.32 -0.0 433,963 + Psyr_0403 0.32 +0.4 433,963 + Psyr_0403 0.32 +1.4 433,963 + Psyr_0403 0.32 -0.7 433,963 + Psyr_0403 0.32 -0.9 433,963 + Psyr_0403 0.32 +3.0 433,964 - Psyr_0403 0.32 -1.3 433,964 - Psyr_0403 0.32 -0.4 433,964 - Psyr_0403 0.32 +0.6 433,964 - Psyr_0403 0.32 -0.1 433,964 - Psyr_0403 0.32 +1.7 433,964 - Psyr_0403 0.32 +2.4 434,272 + Psyr_0403 0.61 +0.3 434,272 + Psyr_0403 0.61 -0.2 434,272 + Psyr_0403 0.61 -0.6 434,272 + Psyr_0403 0.61 -0.4 434,272 + Psyr_0403 0.61 +0.1 434,272 + Psyr_0403 0.61 -1.6 434,272 + Psyr_0403 0.61 -1.3 434,273 - Psyr_0403 0.61 +2.1 434,273 - Psyr_0403 0.61 +0.0 434,273 - Psyr_0403 0.61 +0.4 434,389 + Psyr_0403 0.72 -0.2 434,389 + Psyr_0403 0.72 +0.1 434,389 + Psyr_0403 0.72 +0.9 434,390 - Psyr_0403 0.72 +0.1 434,390 - Psyr_0403 0.72 -1.3 434,390 - Psyr_0403 0.72 +0.4 434,390 - Psyr_0403 0.72 +0.2 434,476 + Psyr_0403 0.80 +0.0 434,476 + Psyr_0403 0.80 +0.0 434,477 - Psyr_0403 0.80 -0.4 434,477 - Psyr_0403 0.80 +1.0 434,477 - Psyr_0403 0.80 -0.3 434,704 + +1.0 434,704 + +0.4 434,704 + -1.0 434,704 + +0.7 434,704 + +0.6 434,704 + +0.6 434,705 - -1.0 434,705 - +0.4 434,705 - +0.6 434,705 - +0.4 434,705 - -0.1 434,705 - -0.9 434,811 + Psyr_0404 0.21 +0.1 434,812 - Psyr_0404 0.22 -2.1 435,027 + Psyr_0404 0.59 +0.2 435,028 - Psyr_0404 0.59 -0.6 435,028 - Psyr_0404 0.59 -1.6 435,028 - Psyr_0404 0.59 +1.1 435,030 + Psyr_0404 0.59 +1.4 435,214 + -0.0 435,265 + -0.4 435,265 + +0.5 435,265 + -2.2 435,265 + -1.4 435,265 + -0.1 435,265 + -0.3 435,265 + -0.5 435,265 + -1.4 435,265 + +1.0 435,265 + -0.2 435,265 + -0.3 435,265 + +1.4 435,265 + -1.0 435,265 + +2.4 435,265 + +0.4 435,265 + -0.6 435,265 + -1.1 435,266 - -0.0 435,266 - -1.5 435,266 - -0.4 435,266 - -1.5 435,266 - -0.2 435,266 - +0.5 435,266 - +0.9 435,266 - +1.8 435,398 + Psyr_0405 0.22 -0.2 435,398 + Psyr_0405 0.22 +0.1 435,398 + Psyr_0405 0.22 +2.4 435,399 - Psyr_0405 0.22 -2.4 435,512 + Psyr_0405 0.40 -1.6 435,513 - Psyr_0405 0.41 -0.8 435,680 + Psyr_0405 0.67 +1.6 435,680 + Psyr_0405 0.67 -0.4 435,680 + Psyr_0405 0.67 -0.5 435,680 + Psyr_0405 0.67 -0.5 435,681 - Psyr_0405 0.67 -0.4
Or see this region's nucleotide sequence