Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS14530

Experiment: Heptanoic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS14520 and ABZR87_RS14525 are separated by 82 nucleotidesABZR87_RS14525 and ABZR87_RS14530 are separated by 80 nucleotidesABZR87_RS14530 and ABZR87_RS14535 are separated by 29 nucleotidesABZR87_RS14535 and ABZR87_RS14540 are separated by 23 nucleotides ABZR87_RS14520: ABZR87_RS14520 - aspartate-semialdehyde dehydrogenase, at 3,068,278 to 3,069,381 _RS14520 ABZR87_RS14525: ABZR87_RS14525 - hypothetical protein, at 3,069,464 to 3,069,640 _RS14525 ABZR87_RS14530: ABZR87_RS14530 - 3-isopropylmalate dehydrogenase, at 3,069,721 to 3,070,791 _RS14530 ABZR87_RS14535: ABZR87_RS14535 - 3-isopropylmalate dehydratase small subunit, at 3,070,821 to 3,071,471 _RS14535 ABZR87_RS14540: ABZR87_RS14540 - 3-isopropylmalate dehydratase large subunit, at 3,071,495 to 3,072,904 _RS14540 Position (kb) 3069 3070 3071Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3069.465 kb on + strandat 3069.582 kb on - strand, within ABZR87_RS14525at 3069.620 kb on + strand, within ABZR87_RS14525at 3069.620 kb on + strand, within ABZR87_RS14525at 3069.804 kb on - strandat 3069.965 kb on + strand, within ABZR87_RS14530at 3069.965 kb on + strand, within ABZR87_RS14530at 3069.965 kb on + strand, within ABZR87_RS14530at 3069.966 kb on - strand, within ABZR87_RS14530at 3069.966 kb on - strand, within ABZR87_RS14530at 3069.966 kb on - strand, within ABZR87_RS14530at 3069.966 kb on - strand, within ABZR87_RS14530at 3069.966 kb on - strand, within ABZR87_RS14530at 3069.966 kb on - strand, within ABZR87_RS14530at 3069.966 kb on - strand, within ABZR87_RS14530at 3069.966 kb on - strand, within ABZR87_RS14530at 3070.130 kb on + strand, within ABZR87_RS14530at 3070.130 kb on + strand, within ABZR87_RS14530at 3070.130 kb on + strand, within ABZR87_RS14530at 3070.130 kb on + strand, within ABZR87_RS14530at 3070.131 kb on - strand, within ABZR87_RS14530at 3070.131 kb on - strand, within ABZR87_RS14530at 3070.131 kb on - strand, within ABZR87_RS14530at 3070.131 kb on - strand, within ABZR87_RS14530at 3070.305 kb on - strand, within ABZR87_RS14530at 3070.385 kb on + strand, within ABZR87_RS14530at 3070.385 kb on + strand, within ABZR87_RS14530at 3070.385 kb on + strand, within ABZR87_RS14530at 3070.386 kb on - strand, within ABZR87_RS14530at 3070.386 kb on - strand, within ABZR87_RS14530at 3070.386 kb on - strand, within ABZR87_RS14530at 3070.386 kb on - strand, within ABZR87_RS14530at 3070.386 kb on - strand, within ABZR87_RS14530at 3070.475 kb on + strand, within ABZR87_RS14530at 3070.571 kb on + strand, within ABZR87_RS14530at 3070.571 kb on + strand, within ABZR87_RS14530at 3070.876 kb on + strandat 3070.876 kb on + strandat 3070.876 kb on + strandat 3070.876 kb on + strandat 3070.877 kb on - strandat 3070.877 kb on - strandat 3070.877 kb on - strandat 3070.897 kb on + strand, within ABZR87_RS14535at 3070.898 kb on - strand, within ABZR87_RS14535at 3070.942 kb on + strand, within ABZR87_RS14535at 3070.943 kb on - strand, within ABZR87_RS14535at 3071.036 kb on + strand, within ABZR87_RS14535at 3071.037 kb on - strand, within ABZR87_RS14535at 3071.167 kb on + strand, within ABZR87_RS14535at 3071.167 kb on + strand, within ABZR87_RS14535at 3071.168 kb on - strand, within ABZR87_RS14535at 3071.168 kb on - strand, within ABZR87_RS14535at 3071.329 kb on + strand, within ABZR87_RS14535at 3071.330 kb on - strand, within ABZR87_RS14535at 3071.373 kb on - strand, within ABZR87_RS14535at 3071.484 kb on + strandat 3071.484 kb on + strandat 3071.484 kb on + strandat 3071.485 kb on - strandat 3071.485 kb on - strandat 3071.562 kb on - strandat 3071.562 kb on - strandat 3071.562 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Heptanoic (C)
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3,069,465 + +0.8
3,069,582 - ABZR87_RS14525 0.67 -1.1
3,069,620 + ABZR87_RS14525 0.88 -0.2
3,069,620 + ABZR87_RS14525 0.88 -1.8
3,069,804 - -3.0
3,069,965 + ABZR87_RS14530 0.23 -3.7
3,069,965 + ABZR87_RS14530 0.23 -3.9
3,069,965 + ABZR87_RS14530 0.23 -2.9
3,069,966 - ABZR87_RS14530 0.23 -3.1
3,069,966 - ABZR87_RS14530 0.23 -4.0
3,069,966 - ABZR87_RS14530 0.23 -4.7
3,069,966 - ABZR87_RS14530 0.23 -2.9
3,069,966 - ABZR87_RS14530 0.23 -4.0
3,069,966 - ABZR87_RS14530 0.23 -2.3
3,069,966 - ABZR87_RS14530 0.23 -3.5
3,069,966 - ABZR87_RS14530 0.23 -1.5
3,070,130 + ABZR87_RS14530 0.38 -3.4
3,070,130 + ABZR87_RS14530 0.38 -4.6
3,070,130 + ABZR87_RS14530 0.38 -3.0
3,070,130 + ABZR87_RS14530 0.38 -4.6
3,070,131 - ABZR87_RS14530 0.38 -0.2
3,070,131 - ABZR87_RS14530 0.38 +0.8
3,070,131 - ABZR87_RS14530 0.38 -3.8
3,070,131 - ABZR87_RS14530 0.38 -2.4
3,070,305 - ABZR87_RS14530 0.55 -3.0
3,070,385 + ABZR87_RS14530 0.62 -2.0
3,070,385 + ABZR87_RS14530 0.62 -4.6
3,070,385 + ABZR87_RS14530 0.62 -3.7
3,070,386 - ABZR87_RS14530 0.62 -2.1
3,070,386 - ABZR87_RS14530 0.62 -4.1
3,070,386 - ABZR87_RS14530 0.62 -4.6
3,070,386 - ABZR87_RS14530 0.62 -3.1
3,070,386 - ABZR87_RS14530 0.62 -2.8
3,070,475 + ABZR87_RS14530 0.70 -3.8
3,070,571 + ABZR87_RS14530 0.79 -1.5
3,070,571 + ABZR87_RS14530 0.79 -0.2
3,070,876 + -4.3
3,070,876 + -2.0
3,070,876 + -4.9
3,070,876 + -3.6
3,070,877 - -3.5
3,070,877 - -2.7
3,070,877 - -4.1
3,070,897 + ABZR87_RS14535 0.12 -4.3
3,070,898 - ABZR87_RS14535 0.12 -4.2
3,070,942 + ABZR87_RS14535 0.19 -3.7
3,070,943 - ABZR87_RS14535 0.19 -4.4
3,071,036 + ABZR87_RS14535 0.33 -2.5
3,071,037 - ABZR87_RS14535 0.33 -4.4
3,071,167 + ABZR87_RS14535 0.53 -3.8
3,071,167 + ABZR87_RS14535 0.53 -1.5
3,071,168 - ABZR87_RS14535 0.53 -3.0
3,071,168 - ABZR87_RS14535 0.53 -3.3
3,071,329 + ABZR87_RS14535 0.78 -3.8
3,071,330 - ABZR87_RS14535 0.78 -4.1
3,071,373 - ABZR87_RS14535 0.85 -3.3
3,071,484 + -2.4
3,071,484 + -2.9
3,071,484 + -0.9
3,071,485 - -0.2
3,071,485 - -0.2
3,071,562 - -4.2
3,071,562 - -2.4
3,071,562 - -4.6

Or see this region's nucleotide sequence