Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02130 and HSERO_RS02135 are separated by 2 nucleotides HSERO_RS02135 and HSERO_RS02140 are separated by 1 nucleotides HSERO_RS02140 and HSERO_RS02145 are separated by 188 nucleotides HSERO_RS02145 and HSERO_RS02150 are separated by 236 nucleotides
HSERO_RS02130: HSERO_RS02130 - thiazole synthase, at 464,921 to 465,730
_RS02130
HSERO_RS02135: HSERO_RS02135 - phosphomethylpyrimidine kinase, at 465,733 to 466,599
_RS02135
HSERO_RS02140: HSERO_RS02140 - thiamine-phosphate synthase, at 466,601 to 467,233
_RS02140
HSERO_RS02145: HSERO_RS02145 - ferritin, at 467,422 to 467,916
_RS02145
HSERO_RS02150: HSERO_RS02150 - MFS transporter, at 468,153 to 469,328
_RS02150
Position (kb) 466
467
468 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 465.821 kb on - strand, within HSERO_RS02135 at 465.869 kb on - strand, within HSERO_RS02135 at 465.885 kb on - strand, within HSERO_RS02135 at 465.895 kb on - strand, within HSERO_RS02135 at 465.925 kb on - strand, within HSERO_RS02135 at 465.953 kb on - strand, within HSERO_RS02135 at 466.006 kb on - strand, within HSERO_RS02135 at 466.197 kb on + strand, within HSERO_RS02135 at 466.263 kb on + strand, within HSERO_RS02135 at 466.474 kb on - strand, within HSERO_RS02135 at 466.485 kb on - strand at 466.487 kb on + strand, within HSERO_RS02135 at 466.567 kb on + strand at 466.682 kb on + strand, within HSERO_RS02140 at 466.803 kb on - strand at 467.172 kb on + strand at 467.210 kb on - strand at 467.264 kb on + strand at 467.264 kb on + strand at 467.264 kb on + strand at 467.348 kb on - strand at 467.808 kb on + strand, within HSERO_RS02145 at 467.923 kb on + strand at 467.931 kb on - strand at 467.953 kb on + strand at 467.955 kb on + strand at 467.961 kb on - strand at 467.963 kb on - strand at 467.963 kb on - strand at 467.963 kb on - strand at 468.104 kb on + strand at 468.151 kb on + strand at 468.170 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 465,821 - HSERO_RS02135 0.10 +1.0 465,869 - HSERO_RS02135 0.16 +0.3 465,885 - HSERO_RS02135 0.18 -2.0 465,895 - HSERO_RS02135 0.19 +0.2 465,925 - HSERO_RS02135 0.22 +0.3 465,953 - HSERO_RS02135 0.25 -0.8 466,006 - HSERO_RS02135 0.31 +0.1 466,197 + HSERO_RS02135 0.54 +0.1 466,263 + HSERO_RS02135 0.61 +0.4 466,474 - HSERO_RS02135 0.85 +1.7 466,485 - +2.0 466,487 + HSERO_RS02135 0.87 +0.0 466,567 + +0.1 466,682 + HSERO_RS02140 0.13 -0.3 466,803 - +0.0 467,172 + +1.2 467,210 - +0.3 467,264 + +0.1 467,264 + +1.2 467,264 + +0.7 467,348 - -0.4 467,808 + HSERO_RS02145 0.78 -4.2 467,923 + -4.7 467,931 - -3.9 467,953 + -2.1 467,955 + -1.7 467,961 - -1.0 467,963 - -0.5 467,963 - +0.4 467,963 - -0.4 468,104 + +1.0 468,151 + -0.9 468,170 + +0.7
Or see this region's nucleotide sequence