Experiment: KB with Terpene 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2224 and Psyr_2225 overlap by 19 nucleotides Psyr_2225 and Psyr_2226 overlap by 4 nucleotides Psyr_2226 and Psyr_2227 are separated by 297 nucleotides
Psyr_2224: Psyr_2224 - FAD dependent oxidoreductase, at 2,585,630 to 2,586,871
_2224
Psyr_2225: Psyr_2225 - methenyltetrahydrofolate cyclohydrolase / 5,10-methylenetetrahydrofolate dehydrogenase (NADP+), at 2,586,853 to 2,587,755
_2225
Psyr_2226: Psyr_2226 - formyltetrahydrofolate deformylase, at 2,587,752 to 2,588,618
_2226
Psyr_2227: Psyr_2227 - conserved hypothetical protein, at 2,588,916 to 2,589,371
_2227
Position (kb)
2587
2588
2589 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2586.857 kb on + strand at 2586.948 kb on + strand, within Psyr_2225 at 2587.349 kb on - strand, within Psyr_2225 at 2587.607 kb on - strand, within Psyr_2225 at 2587.607 kb on - strand, within Psyr_2225 at 2587.610 kb on + strand, within Psyr_2225 at 2587.610 kb on + strand, within Psyr_2225 at 2587.610 kb on + strand, within Psyr_2225 at 2587.807 kb on + strand at 2587.807 kb on + strand at 2587.807 kb on + strand at 2587.808 kb on - strand at 2587.808 kb on - strand at 2587.830 kb on + strand at 2587.942 kb on + strand at 2587.943 kb on - strand, within Psyr_2226 at 2588.188 kb on + strand, within Psyr_2226 at 2588.188 kb on + strand, within Psyr_2226 at 2588.189 kb on - strand, within Psyr_2226 at 2588.485 kb on + strand, within Psyr_2226 at 2588.486 kb on - strand at 2588.486 kb on - strand, within Psyr_2226 at 2588.518 kb on + strand, within Psyr_2226 at 2588.519 kb on - strand, within Psyr_2226 at 2588.519 kb on - strand, within Psyr_2226 at 2588.736 kb on + strand at 2588.736 kb on + strand at 2588.736 kb on + strand at 2588.736 kb on + strand at 2588.737 kb on - strand at 2588.737 kb on - strand at 2588.737 kb on - strand at 2588.889 kb on - strand at 2588.889 kb on - strand at 2588.918 kb on - strand at 2588.918 kb on - strand at 2588.918 kb on - strand at 2588.967 kb on - strand, within Psyr_2227 at 2588.987 kb on + strand, within Psyr_2227 at 2589.282 kb on + strand, within Psyr_2227 at 2589.332 kb on + strand at 2589.332 kb on + strand at 2589.409 kb on + strand at 2589.410 kb on - strand at 2589.418 kb on + strand at 2589.443 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.454 kb on - strand at 2589.454 kb on - strand at 2589.457 kb on + strand at 2589.457 kb on + strand at 2589.474 kb on + strand at 2589.477 kb on - strand at 2589.521 kb on + strand at 2589.521 kb on + strand at 2589.522 kb on - strand at 2589.522 kb on - strand at 2589.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Terpene 10 mM remove 2,586,857 + +1.4 2,586,948 + Psyr_2225 0.11 +0.6 2,587,349 - Psyr_2225 0.55 -0.2 2,587,607 - Psyr_2225 0.83 +0.8 2,587,607 - Psyr_2225 0.83 +1.6 2,587,610 + Psyr_2225 0.84 -1.0 2,587,610 + Psyr_2225 0.84 +1.2 2,587,610 + Psyr_2225 0.84 +0.5 2,587,807 + -0.9 2,587,807 + -0.4 2,587,807 + -0.6 2,587,808 - -0.7 2,587,808 - +0.2 2,587,830 + +1.2 2,587,942 + +0.2 2,587,943 - Psyr_2226 0.22 -1.4 2,588,188 + Psyr_2226 0.50 -0.6 2,588,188 + Psyr_2226 0.50 -0.3 2,588,189 - Psyr_2226 0.50 -0.0 2,588,485 + Psyr_2226 0.85 -0.6 2,588,486 - -1.4 2,588,486 - Psyr_2226 0.85 -0.5 2,588,518 + Psyr_2226 0.88 -0.6 2,588,519 - Psyr_2226 0.88 -0.3 2,588,519 - Psyr_2226 0.88 +0.4 2,588,736 + +0.7 2,588,736 + -2.8 2,588,736 + +1.2 2,588,736 + +0.7 2,588,737 - -0.5 2,588,737 - +0.7 2,588,737 - -1.1 2,588,889 - -0.7 2,588,889 - -0.2 2,588,918 - -0.5 2,588,918 - +0.6 2,588,918 - -1.3 2,588,967 - Psyr_2227 0.11 +0.3 2,588,987 + Psyr_2227 0.16 -2.4 2,589,282 + Psyr_2227 0.80 +0.6 2,589,332 + -1.9 2,589,332 + +0.4 2,589,409 + -0.2 2,589,410 - -1.6 2,589,418 + -0.2 2,589,443 + +0.1 2,589,453 + +0.2 2,589,453 + +0.2 2,589,453 + +0.5 2,589,453 + -2.4 2,589,454 - +0.8 2,589,454 - -1.3 2,589,457 + -0.4 2,589,457 + -0.1 2,589,474 + +0.2 2,589,477 - -2.1 2,589,521 + +0.2 2,589,521 + -0.6 2,589,522 - +0.6 2,589,522 - +1.5 2,589,522 - -0.2
Or see this region's nucleotide sequence