Experiment: BHIS with Cephalothin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4749 and BT4750 are separated by 111 nucleotides BT4750 and BT4751 are separated by 13 nucleotides BT4751 and BT4752 are separated by 111 nucleotides
BT4749: BT4749 - putative DNA methylase (NCBI ptt file), at 6,231,192 to 6,235,154
BT4749
BT4750: BT4750 - hypothetical protein (NCBI ptt file), at 6,235,266 to 6,235,463
BT4750
BT4751: BT4751 - hypothetical protein (NCBI ptt file), at 6,235,477 to 6,235,920
BT4751
BT4752: BT4752 - conserved hypothetical protein (NCBI ptt file), at 6,236,032 to 6,237,327
BT4752
Position (kb)
6235
6236 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6234.491 kb on + strand, within BT4749 at 6234.492 kb on - strand, within BT4749 at 6234.529 kb on + strand, within BT4749 at 6234.560 kb on + strand, within BT4749 at 6234.560 kb on + strand, within BT4749 at 6234.561 kb on - strand, within BT4749 at 6234.575 kb on + strand, within BT4749 at 6234.776 kb on - strand at 6234.826 kb on + strand at 6234.831 kb on - strand at 6234.917 kb on + strand at 6234.945 kb on - strand at 6234.947 kb on + strand at 6234.948 kb on - strand at 6234.950 kb on - strand at 6234.952 kb on + strand at 6234.953 kb on - strand at 6234.960 kb on + strand at 6234.978 kb on + strand at 6235.059 kb on + strand at 6235.180 kb on + strand at 6235.249 kb on + strand at 6235.479 kb on - strand at 6235.573 kb on - strand, within BT4751 at 6235.589 kb on - strand, within BT4751 at 6235.609 kb on + strand, within BT4751 at 6235.766 kb on - strand, within BT4751 at 6235.778 kb on + strand, within BT4751 at 6235.912 kb on - strand at 6235.931 kb on + strand at 6235.944 kb on + strand at 6235.968 kb on - strand at 6235.998 kb on + strand at 6236.000 kb on + strand at 6236.033 kb on + strand at 6236.078 kb on - strand at 6236.167 kb on + strand, within BT4752 at 6236.200 kb on + strand, within BT4752 at 6236.419 kb on + strand, within BT4752 at 6236.419 kb on + strand, within BT4752 at 6236.494 kb on - strand, within BT4752 at 6236.495 kb on + strand, within BT4752 at 6236.564 kb on + strand, within BT4752 at 6236.565 kb on - strand, within BT4752 at 6236.565 kb on - strand, within BT4752 at 6236.565 kb on - strand, within BT4752 at 6236.659 kb on - strand, within BT4752 at 6236.673 kb on - strand, within BT4752 at 6236.805 kb on + strand, within BT4752
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.1 mM remove 6,234,491 + BT4749 0.83 +0.0 6,234,492 - BT4749 0.83 -2.8 6,234,529 + BT4749 0.84 -2.3 6,234,560 + BT4749 0.85 +1.3 6,234,560 + BT4749 0.85 -1.0 6,234,561 - BT4749 0.85 +0.0 6,234,575 + BT4749 0.85 -2.3 6,234,776 - -2.4 6,234,826 + -1.7 6,234,831 - -0.7 6,234,917 + +0.0 6,234,945 - +0.0 6,234,947 + +0.0 6,234,948 - +2.1 6,234,950 - -2.3 6,234,952 + -1.3 6,234,953 - +0.0 6,234,960 + +0.0 6,234,978 + +0.0 6,235,059 + -1.4 6,235,180 + -1.0 6,235,249 + -2.6 6,235,479 - -1.2 6,235,573 - BT4751 0.22 -1.4 6,235,589 - BT4751 0.25 -2.3 6,235,609 + BT4751 0.30 -3.6 6,235,766 - BT4751 0.65 -3.8 6,235,778 + BT4751 0.68 -1.5 6,235,912 - -1.0 6,235,931 + +3.0 6,235,944 + -1.3 6,235,968 - -1.6 6,235,998 + +0.0 6,236,000 + -1.6 6,236,033 + -1.6 6,236,078 - +0.0 6,236,167 + BT4752 0.10 -1.0 6,236,200 + BT4752 0.13 -0.9 6,236,419 + BT4752 0.30 +1.0 6,236,419 + BT4752 0.30 -1.3 6,236,494 - BT4752 0.36 -2.1 6,236,495 + BT4752 0.36 -1.1 6,236,564 + BT4752 0.41 -0.3 6,236,565 - BT4752 0.41 +0.5 6,236,565 - BT4752 0.41 -1.6 6,236,565 - BT4752 0.41 +1.5 6,236,659 - BT4752 0.48 +1.2 6,236,673 - BT4752 0.49 +2.3 6,236,805 + BT4752 0.60 -1.8
Or see this region's nucleotide sequence