Experiment: BHIS with Cephalothin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3750 and BT3751 are separated by 61 nucleotides BT3751 and BT3752 are separated by 13 nucleotides
BT3750: BT3750 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 4,867,933 to 4,870,533
BT3750
BT3751: BT3751 - outer membrane protein (NCBI ptt file), at 4,870,595 to 4,871,266
BT3751
BT3752: BT3752 - hypothetical protein (NCBI ptt file), at 4,871,280 to 4,872,845
BT3752
Position (kb)
4870
4871
4872 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4869.614 kb on - strand, within BT3750 at 4869.660 kb on + strand, within BT3750 at 4869.664 kb on + strand, within BT3750 at 4869.678 kb on + strand, within BT3750 at 4869.683 kb on - strand, within BT3750 at 4869.685 kb on - strand, within BT3750 at 4869.692 kb on - strand, within BT3750 at 4869.734 kb on - strand, within BT3750 at 4869.748 kb on - strand, within BT3750 at 4869.748 kb on - strand, within BT3750 at 4869.767 kb on - strand, within BT3750 at 4869.854 kb on - strand, within BT3750 at 4870.007 kb on - strand, within BT3750 at 4870.067 kb on - strand, within BT3750 at 4870.123 kb on - strand, within BT3750 at 4870.165 kb on + strand, within BT3750 at 4870.166 kb on - strand, within BT3750 at 4870.175 kb on + strand, within BT3750 at 4870.176 kb on - strand, within BT3750 at 4870.176 kb on - strand, within BT3750 at 4870.214 kb on - strand, within BT3750 at 4870.214 kb on - strand, within BT3750 at 4870.214 kb on - strand, within BT3750 at 4870.304 kb on + strand at 4870.341 kb on + strand at 4870.352 kb on - strand at 4870.365 kb on + strand at 4870.385 kb on - strand at 4870.415 kb on - strand at 4870.416 kb on + strand at 4870.417 kb on - strand at 4870.475 kb on - strand at 4870.532 kb on - strand at 4870.565 kb on + strand at 4870.566 kb on - strand at 4870.616 kb on - strand at 4870.619 kb on + strand at 4870.628 kb on - strand at 4870.675 kb on - strand, within BT3751 at 4870.675 kb on - strand, within BT3751 at 4870.677 kb on - strand, within BT3751 at 4870.768 kb on - strand, within BT3751 at 4870.818 kb on - strand, within BT3751 at 4870.820 kb on - strand, within BT3751 at 4870.983 kb on - strand, within BT3751 at 4871.012 kb on + strand, within BT3751 at 4871.065 kb on + strand, within BT3751 at 4871.066 kb on - strand, within BT3751 at 4871.092 kb on + strand, within BT3751 at 4871.214 kb on - strand at 4871.214 kb on - strand at 4871.214 kb on - strand at 4871.216 kb on - strand at 4871.216 kb on - strand at 4871.216 kb on - strand at 4871.252 kb on - strand at 4871.272 kb on - strand at 4871.272 kb on - strand at 4871.276 kb on - strand at 4871.276 kb on - strand at 4871.321 kb on - strand at 4871.325 kb on - strand at 4871.340 kb on - strand at 4871.404 kb on + strand at 4871.428 kb on - strand at 4871.528 kb on + strand, within BT3752 at 4871.587 kb on - strand, within BT3752 at 4871.592 kb on + strand, within BT3752 at 4871.594 kb on + strand, within BT3752 at 4871.595 kb on - strand, within BT3752 at 4871.598 kb on + strand, within BT3752 at 4871.598 kb on + strand, within BT3752 at 4871.599 kb on - strand, within BT3752 at 4871.718 kb on + strand, within BT3752 at 4871.755 kb on - strand, within BT3752 at 4871.773 kb on + strand, within BT3752 at 4871.773 kb on + strand, within BT3752 at 4871.875 kb on - strand, within BT3752 at 4871.912 kb on + strand, within BT3752 at 4871.950 kb on + strand, within BT3752 at 4871.961 kb on + strand, within BT3752 at 4871.962 kb on - strand, within BT3752 at 4871.990 kb on - strand, within BT3752 at 4872.028 kb on - strand, within BT3752 at 4872.069 kb on - strand, within BT3752 at 4872.069 kb on - strand, within BT3752 at 4872.069 kb on - strand, within BT3752 at 4872.117 kb on + strand, within BT3752 at 4872.118 kb on - strand, within BT3752 at 4872.193 kb on + strand, within BT3752 at 4872.193 kb on + strand, within BT3752 at 4872.193 kb on + strand, within BT3752 at 4872.193 kb on + strand, within BT3752 at 4872.194 kb on - strand, within BT3752 at 4872.230 kb on + strand, within BT3752
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.1 mM remove 4,869,614 - BT3750 0.65 -0.8 4,869,660 + BT3750 0.66 +0.7 4,869,664 + BT3750 0.67 -0.5 4,869,678 + BT3750 0.67 -1.3 4,869,683 - BT3750 0.67 -0.0 4,869,685 - BT3750 0.67 -1.3 4,869,692 - BT3750 0.68 -3.2 4,869,734 - BT3750 0.69 -1.0 4,869,748 - BT3750 0.70 -2.3 4,869,748 - BT3750 0.70 -2.5 4,869,767 - BT3750 0.71 -1.4 4,869,854 - BT3750 0.74 -0.6 4,870,007 - BT3750 0.80 -3.4 4,870,067 - BT3750 0.82 -0.2 4,870,123 - BT3750 0.84 -0.2 4,870,165 + BT3750 0.86 +1.3 4,870,166 - BT3750 0.86 -2.3 4,870,175 + BT3750 0.86 -2.6 4,870,176 - BT3750 0.86 -2.7 4,870,176 - BT3750 0.86 -0.2 4,870,214 - BT3750 0.88 +0.6 4,870,214 - BT3750 0.88 +2.6 4,870,214 - BT3750 0.88 -1.9 4,870,304 + -2.7 4,870,341 + -0.0 4,870,352 - -1.0 4,870,365 + +1.6 4,870,385 - -1.3 4,870,415 - -1.1 4,870,416 + +0.3 4,870,417 - -1.3 4,870,475 - -0.8 4,870,532 - -0.9 4,870,565 + -1.2 4,870,566 - +1.2 4,870,616 - -0.0 4,870,619 + -2.1 4,870,628 - -2.8 4,870,675 - BT3751 0.12 -2.2 4,870,675 - BT3751 0.12 -1.0 4,870,677 - BT3751 0.12 -0.6 4,870,768 - BT3751 0.26 -2.8 4,870,818 - BT3751 0.33 -1.7 4,870,820 - BT3751 0.33 -1.9 4,870,983 - BT3751 0.58 -2.3 4,871,012 + BT3751 0.62 -3.9 4,871,065 + BT3751 0.70 -0.4 4,871,066 - BT3751 0.70 -1.6 4,871,092 + BT3751 0.74 -2.9 4,871,214 - -1.1 4,871,214 - -1.0 4,871,214 - -3.3 4,871,216 - -1.6 4,871,216 - +2.3 4,871,216 - -2.0 4,871,252 - -2.3 4,871,272 - -1.0 4,871,272 - -1.9 4,871,276 - -1.3 4,871,276 - -0.0 4,871,321 - -0.1 4,871,325 - -2.3 4,871,340 - -1.4 4,871,404 + -2.0 4,871,428 - -2.8 4,871,528 + BT3752 0.16 -1.0 4,871,587 - BT3752 0.20 -0.0 4,871,592 + BT3752 0.20 -1.6 4,871,594 + BT3752 0.20 -1.6 4,871,595 - BT3752 0.20 +0.6 4,871,598 + BT3752 0.20 -3.1 4,871,598 + BT3752 0.20 -2.0 4,871,599 - BT3752 0.20 -0.6 4,871,718 + BT3752 0.28 +0.6 4,871,755 - BT3752 0.30 -1.3 4,871,773 + BT3752 0.31 -3.2 4,871,773 + BT3752 0.31 -0.0 4,871,875 - BT3752 0.38 -0.6 4,871,912 + BT3752 0.40 -1.0 4,871,950 + BT3752 0.43 +1.0 4,871,961 + BT3752 0.43 -1.8 4,871,962 - BT3752 0.44 -2.5 4,871,990 - BT3752 0.45 -0.2 4,872,028 - BT3752 0.48 -1.0 4,872,069 - BT3752 0.50 -1.2 4,872,069 - BT3752 0.50 -1.6 4,872,069 - BT3752 0.50 -0.0 4,872,117 + BT3752 0.53 -3.0 4,872,118 - BT3752 0.54 -2.3 4,872,193 + BT3752 0.58 -3.2 4,872,193 + BT3752 0.58 -0.0 4,872,193 + BT3752 0.58 -0.3 4,872,193 + BT3752 0.58 -2.0 4,872,194 - BT3752 0.58 -0.7 4,872,230 + BT3752 0.61 +0.4
Or see this region's nucleotide sequence