Experiment: BHIS with Cephalothin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3480 and BT3481 are separated by 431 nucleotides BT3481 and BT3482 are separated by 17 nucleotides BT3482 and BT3483 are separated by 11 nucleotides
BT3480: BT3480 - conserved hypothetical protein (NCBI ptt file), at 4,485,652 to 4,486,035
BT3480
BT3481: BT3481 - putative glutaminase A (NCBI ptt file), at 4,486,467 to 4,486,991
BT3481
BT3482: BT3482 - conserved hypothetical protein (NCBI ptt file), at 4,487,009 to 4,488,550
BT3482
BT3483: BT3483 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 4,488,562 to 4,491,702
BT3483
Position (kb)
4487
4488
4489 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4486.077 kb on + strand at 4486.099 kb on + strand at 4486.100 kb on - strand at 4486.129 kb on - strand at 4486.148 kb on + strand at 4486.148 kb on + strand at 4486.148 kb on + strand at 4486.161 kb on + strand at 4486.179 kb on - strand at 4486.235 kb on - strand at 4486.246 kb on - strand at 4486.261 kb on + strand at 4486.295 kb on + strand at 4486.298 kb on - strand at 4486.315 kb on + strand at 4486.316 kb on - strand at 4486.832 kb on - strand, within BT3481 at 4486.902 kb on - strand, within BT3481 at 4486.940 kb on - strand at 4487.006 kb on - strand at 4487.050 kb on - strand at 4487.054 kb on + strand at 4487.055 kb on - strand at 4487.112 kb on + strand at 4487.112 kb on + strand at 4487.129 kb on - strand at 4487.177 kb on - strand, within BT3482 at 4487.186 kb on - strand, within BT3482 at 4487.220 kb on - strand, within BT3482 at 4487.307 kb on - strand, within BT3482 at 4487.307 kb on - strand, within BT3482 at 4487.308 kb on + strand, within BT3482 at 4487.309 kb on - strand, within BT3482 at 4487.362 kb on - strand, within BT3482 at 4487.531 kb on - strand, within BT3482 at 4487.562 kb on - strand, within BT3482 at 4487.590 kb on + strand, within BT3482 at 4487.659 kb on - strand, within BT3482 at 4487.663 kb on - strand, within BT3482 at 4487.663 kb on - strand, within BT3482 at 4487.666 kb on - strand, within BT3482 at 4487.747 kb on - strand, within BT3482 at 4487.792 kb on + strand, within BT3482 at 4487.868 kb on - strand, within BT3482 at 4487.913 kb on + strand, within BT3482 at 4487.945 kb on + strand, within BT3482 at 4488.026 kb on + strand, within BT3482 at 4488.027 kb on - strand, within BT3482 at 4488.027 kb on - strand, within BT3482 at 4488.029 kb on - strand, within BT3482 at 4488.029 kb on - strand, within BT3482 at 4488.047 kb on - strand, within BT3482 at 4488.062 kb on - strand, within BT3482 at 4488.067 kb on - strand, within BT3482 at 4488.109 kb on + strand, within BT3482 at 4488.139 kb on + strand, within BT3482 at 4488.266 kb on - strand, within BT3482 at 4488.407 kb on + strand at 4488.408 kb on - strand at 4488.489 kb on - strand at 4488.489 kb on - strand at 4488.489 kb on - strand at 4488.504 kb on - strand at 4488.586 kb on - strand at 4488.685 kb on - strand at 4488.685 kb on - strand at 4488.764 kb on - strand at 4488.787 kb on - strand at 4488.837 kb on + strand at 4488.839 kb on + strand at 4488.930 kb on + strand, within BT3483 at 4488.931 kb on - strand, within BT3483 at 4488.944 kb on + strand, within BT3483 at 4488.985 kb on - strand, within BT3483 at 4489.041 kb on + strand, within BT3483 at 4489.093 kb on - strand, within BT3483 at 4489.294 kb on + strand, within BT3483 at 4489.299 kb on + strand, within BT3483 at 4489.365 kb on + strand, within BT3483 at 4489.378 kb on + strand, within BT3483 at 4489.500 kb on - strand, within BT3483 at 4489.538 kb on - strand, within BT3483
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.1 mM remove 4,486,077 + -1.5 4,486,099 + +2.2 4,486,100 - +0.6 4,486,129 - -1.2 4,486,148 + +1.4 4,486,148 + +0.6 4,486,148 + -2.1 4,486,161 + -1.1 4,486,179 - +0.1 4,486,235 - -2.4 4,486,246 - -0.1 4,486,261 + -2.4 4,486,295 + -1.7 4,486,298 - -0.1 4,486,315 + -2.7 4,486,316 - -1.3 4,486,832 - BT3481 0.70 -1.1 4,486,902 - BT3481 0.83 -3.6 4,486,940 - -0.8 4,487,006 - -2.1 4,487,050 - +1.8 4,487,054 + -0.9 4,487,055 - -0.5 4,487,112 + -2.1 4,487,112 + -0.1 4,487,129 - -1.6 4,487,177 - BT3482 0.11 -0.7 4,487,186 - BT3482 0.11 -0.1 4,487,220 - BT3482 0.14 -1.3 4,487,307 - BT3482 0.19 -2.1 4,487,307 - BT3482 0.19 -1.1 4,487,308 + BT3482 0.19 -3.4 4,487,309 - BT3482 0.19 -1.1 4,487,362 - BT3482 0.23 -1.0 4,487,531 - BT3482 0.34 -1.3 4,487,562 - BT3482 0.36 -2.1 4,487,590 + BT3482 0.38 -0.1 4,487,659 - BT3482 0.42 -2.1 4,487,663 - BT3482 0.42 -4.6 4,487,663 - BT3482 0.42 -2.1 4,487,666 - BT3482 0.43 -2.7 4,487,747 - BT3482 0.48 -2.7 4,487,792 + BT3482 0.51 -0.1 4,487,868 - BT3482 0.56 -2.3 4,487,913 + BT3482 0.59 +0.9 4,487,945 + BT3482 0.61 -0.6 4,488,026 + BT3482 0.66 -2.4 4,488,027 - BT3482 0.66 -1.9 4,488,027 - BT3482 0.66 -2.7 4,488,029 - BT3482 0.66 -0.7 4,488,029 - BT3482 0.66 -2.9 4,488,047 - BT3482 0.67 -3.1 4,488,062 - BT3482 0.68 -0.1 4,488,067 - BT3482 0.69 -0.1 4,488,109 + BT3482 0.71 -1.1 4,488,139 + BT3482 0.73 -2.9 4,488,266 - BT3482 0.82 -1.1 4,488,407 + -1.7 4,488,408 - -1.1 4,488,489 - -3.3 4,488,489 - -2.0 4,488,489 - -3.3 4,488,504 - -0.1 4,488,586 - -0.1 4,488,685 - -1.3 4,488,685 - -0.7 4,488,764 - -1.1 4,488,787 - +0.4 4,488,837 + -0.1 4,488,839 + -0.1 4,488,930 + BT3483 0.12 +1.1 4,488,931 - BT3483 0.12 -2.9 4,488,944 + BT3483 0.12 -0.3 4,488,985 - BT3483 0.13 -0.1 4,489,041 + BT3483 0.15 -0.1 4,489,093 - BT3483 0.17 -2.8 4,489,294 + BT3483 0.23 -2.9 4,489,299 + BT3483 0.23 -0.7 4,489,365 + BT3483 0.26 -0.1 4,489,378 + BT3483 0.26 -2.9 4,489,500 - BT3483 0.30 -4.5 4,489,538 - BT3483 0.31 -1.9
Or see this region's nucleotide sequence