Experiment: BHIS with Cephalothin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2430 and BT2431 are separated by 258 nucleotides BT2431 and BT2432 are separated by 62 nucleotides
BT2430: BT2430 - hexokinase type III (NCBI ptt file), at 3,040,198 to 3,041,406
BT2430
BT2431: BT2431 - conserved hypothetical protein (NCBI ptt file), at 3,041,665 to 3,043,089
BT2431
BT2432: BT2432 - beta-mannosidase precursor (NCBI ptt file), at 3,043,152 to 3,046,085
BT2432
Position (kb)
3041
3042
3043
3044 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3040.702 kb on - strand, within BT2430 at 3040.735 kb on - strand, within BT2430 at 3040.767 kb on + strand, within BT2430 at 3040.772 kb on - strand, within BT2430 at 3040.781 kb on - strand, within BT2430 at 3040.781 kb on - strand, within BT2430 at 3040.998 kb on + strand, within BT2430 at 3040.999 kb on - strand, within BT2430 at 3041.002 kb on + strand, within BT2430 at 3041.002 kb on + strand, within BT2430 at 3041.111 kb on + strand, within BT2430 at 3041.113 kb on + strand, within BT2430 at 3041.114 kb on - strand, within BT2430 at 3041.114 kb on - strand, within BT2430 at 3041.118 kb on - strand, within BT2430 at 3041.123 kb on + strand, within BT2430 at 3041.124 kb on - strand, within BT2430 at 3041.138 kb on - strand, within BT2430 at 3041.214 kb on - strand, within BT2430 at 3041.405 kb on - strand at 3041.407 kb on + strand at 3041.407 kb on + strand at 3041.441 kb on + strand at 3041.468 kb on - strand at 3041.486 kb on + strand at 3041.505 kb on + strand at 3041.514 kb on - strand at 3041.517 kb on + strand at 3041.547 kb on + strand at 3041.548 kb on - strand at 3041.560 kb on - strand at 3041.594 kb on + strand at 3041.596 kb on + strand at 3041.596 kb on + strand at 3041.597 kb on - strand at 3041.626 kb on + strand at 3041.643 kb on + strand at 3041.664 kb on + strand at 3041.671 kb on + strand at 3041.770 kb on - strand at 3041.770 kb on - strand at 3041.776 kb on - strand at 3041.779 kb on + strand at 3041.779 kb on + strand at 3041.779 kb on + strand at 3041.779 kb on + strand at 3041.779 kb on + strand at 3041.780 kb on - strand at 3041.780 kb on - strand at 3041.800 kb on - strand at 3041.821 kb on + strand, within BT2431 at 3041.888 kb on - strand, within BT2431 at 3041.981 kb on + strand, within BT2431 at 3042.025 kb on - strand, within BT2431 at 3042.025 kb on - strand, within BT2431 at 3042.049 kb on + strand, within BT2431 at 3042.050 kb on - strand, within BT2431 at 3042.079 kb on + strand, within BT2431 at 3042.096 kb on + strand, within BT2431 at 3042.103 kb on + strand, within BT2431 at 3042.103 kb on + strand, within BT2431 at 3042.115 kb on - strand, within BT2431 at 3042.115 kb on - strand, within BT2431 at 3042.195 kb on + strand, within BT2431 at 3042.196 kb on - strand, within BT2431 at 3042.196 kb on - strand, within BT2431 at 3042.204 kb on - strand, within BT2431 at 3042.218 kb on + strand, within BT2431 at 3042.308 kb on - strand, within BT2431 at 3042.454 kb on - strand, within BT2431 at 3042.461 kb on - strand, within BT2431 at 3042.463 kb on + strand, within BT2431 at 3042.503 kb on + strand, within BT2431 at 3042.667 kb on - strand, within BT2431 at 3042.667 kb on - strand, within BT2431 at 3042.847 kb on + strand, within BT2431 at 3042.924 kb on + strand, within BT2431 at 3042.925 kb on - strand, within BT2431 at 3042.995 kb on + strand at 3042.996 kb on - strand at 3043.101 kb on - strand at 3043.102 kb on + strand at 3043.102 kb on + strand at 3043.103 kb on - strand at 3043.109 kb on - strand at 3043.153 kb on + strand at 3043.176 kb on + strand at 3043.184 kb on - strand at 3043.188 kb on - strand at 3043.233 kb on - strand at 3043.262 kb on + strand at 3043.284 kb on + strand at 3043.287 kb on - strand at 3043.367 kb on + strand at 3043.386 kb on - strand at 3043.433 kb on + strand at 3043.434 kb on - strand at 3043.434 kb on - strand at 3043.445 kb on + strand at 3043.460 kb on + strand, within BT2432 at 3043.573 kb on - strand, within BT2432 at 3043.604 kb on + strand, within BT2432 at 3043.604 kb on + strand, within BT2432 at 3043.604 kb on + strand, within BT2432 at 3043.604 kb on + strand, within BT2432 at 3043.604 kb on + strand, within BT2432 at 3043.604 kb on + strand, within BT2432 at 3043.604 kb on + strand, within BT2432 at 3043.605 kb on - strand, within BT2432 at 3043.636 kb on + strand, within BT2432 at 3043.682 kb on + strand, within BT2432 at 3043.716 kb on + strand, within BT2432 at 3043.789 kb on - strand, within BT2432 at 3043.816 kb on + strand, within BT2432 at 3043.816 kb on + strand, within BT2432 at 3043.817 kb on - strand, within BT2432 at 3043.973 kb on - strand, within BT2432 at 3043.982 kb on + strand, within BT2432 at 3044.054 kb on + strand, within BT2432 at 3044.067 kb on - strand, within BT2432
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.1 mM remove 3,040,702 - BT2430 0.42 -0.6 3,040,735 - BT2430 0.44 -0.1 3,040,767 + BT2430 0.47 +1.5 3,040,772 - BT2430 0.47 -1.6 3,040,781 - BT2430 0.48 +0.8 3,040,781 - BT2430 0.48 -2.4 3,040,998 + BT2430 0.66 -1.6 3,040,999 - BT2430 0.66 +1.5 3,041,002 + BT2430 0.67 -2.9 3,041,002 + BT2430 0.67 -0.1 3,041,111 + BT2430 0.76 -0.1 3,041,113 + BT2430 0.76 +2.3 3,041,114 - BT2430 0.76 +0.9 3,041,114 - BT2430 0.76 -1.1 3,041,118 - BT2430 0.76 +1.3 3,041,123 + BT2430 0.77 -2.2 3,041,124 - BT2430 0.77 -2.6 3,041,138 - BT2430 0.78 +2.3 3,041,214 - BT2430 0.84 -1.6 3,041,405 - +1.1 3,041,407 + -0.6 3,041,407 + -2.1 3,041,441 + -0.4 3,041,468 - +2.3 3,041,486 + -0.1 3,041,505 + -2.1 3,041,514 - -2.1 3,041,517 + +0.3 3,041,547 + -0.8 3,041,548 - -0.7 3,041,560 - -1.1 3,041,594 + -1.3 3,041,596 + +0.4 3,041,596 + -0.1 3,041,597 - -2.1 3,041,626 + -0.5 3,041,643 + -1.8 3,041,664 + -1.1 3,041,671 + -1.1 3,041,770 - -2.1 3,041,770 - -4.4 3,041,776 - -0.2 3,041,779 + -3.3 3,041,779 + +0.2 3,041,779 + -2.6 3,041,779 + -1.1 3,041,779 + -1.6 3,041,780 - +1.3 3,041,780 - +1.5 3,041,800 - -1.6 3,041,821 + BT2431 0.11 -1.7 3,041,888 - BT2431 0.16 -1.1 3,041,981 + BT2431 0.22 -0.7 3,042,025 - BT2431 0.25 -1.1 3,042,025 - BT2431 0.25 -1.6 3,042,049 + BT2431 0.27 -2.1 3,042,050 - BT2431 0.27 -1.5 3,042,079 + BT2431 0.29 -2.4 3,042,096 + BT2431 0.30 -2.1 3,042,103 + BT2431 0.31 -2.4 3,042,103 + BT2431 0.31 -1.4 3,042,115 - BT2431 0.32 -2.4 3,042,115 - BT2431 0.32 +0.9 3,042,195 + BT2431 0.37 -1.0 3,042,196 - BT2431 0.37 -0.5 3,042,196 - BT2431 0.37 +0.4 3,042,204 - BT2431 0.38 -3.0 3,042,218 + BT2431 0.39 -1.8 3,042,308 - BT2431 0.45 -0.1 3,042,454 - BT2431 0.55 -1.1 3,042,461 - BT2431 0.56 -2.4 3,042,463 + BT2431 0.56 -1.1 3,042,503 + BT2431 0.59 -2.8 3,042,667 - BT2431 0.70 -0.4 3,042,667 - BT2431 0.70 +2.5 3,042,847 + BT2431 0.83 +1.5 3,042,924 + BT2431 0.88 -3.7 3,042,925 - BT2431 0.88 -0.8 3,042,995 + -1.7 3,042,996 - -1.6 3,043,101 - -0.7 3,043,102 + +0.5 3,043,102 + -2.1 3,043,103 - -1.7 3,043,109 - +0.4 3,043,153 + -1.0 3,043,176 + -0.6 3,043,184 - -0.5 3,043,188 - -1.0 3,043,233 - -2.6 3,043,262 + -0.1 3,043,284 + -1.6 3,043,287 - +2.1 3,043,367 + -1.2 3,043,386 - -2.4 3,043,433 + -0.7 3,043,434 - -0.1 3,043,434 - -0.3 3,043,445 + -1.1 3,043,460 + BT2432 0.10 +1.6 3,043,573 - BT2432 0.14 -0.1 3,043,604 + BT2432 0.15 -2.6 3,043,604 + BT2432 0.15 -0.9 3,043,604 + BT2432 0.15 -1.5 3,043,604 + BT2432 0.15 -3.0 3,043,604 + BT2432 0.15 -1.6 3,043,604 + BT2432 0.15 -1.6 3,043,604 + BT2432 0.15 -1.1 3,043,605 - BT2432 0.15 +3.5 3,043,636 + BT2432 0.16 -3.1 3,043,682 + BT2432 0.18 -1.0 3,043,716 + BT2432 0.19 -1.1 3,043,789 - BT2432 0.22 -2.1 3,043,816 + BT2432 0.23 +0.5 3,043,816 + BT2432 0.23 -3.1 3,043,817 - BT2432 0.23 -2.4 3,043,973 - BT2432 0.28 +2.3 3,043,982 + BT2432 0.28 +2.9 3,044,054 + BT2432 0.31 -2.6 3,044,067 - BT2432 0.31 +0.8
Or see this region's nucleotide sequence