Experiment: BHIS with Cephalothin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1751 and BT1752 are separated by 604 nucleotides BT1752 and BT1753 overlap by 38 nucleotides BT1753 and BT1754 are separated by 27 nucleotides
BT1751: BT1751 - Glycine betaine transport ATP-binding protein (NCBI ptt file), at 2,161,451 to 2,162,677
BT1751
BT1752: BT1752 - hypothetical protein (NCBI ptt file), at 2,163,282 to 2,164,436
BT1752
BT1753: BT1753 - putative methylated-DNA methyltransferase (NCBI ptt file), at 2,164,399 to 2,164,731
BT1753
BT1754: BT1754 - two-component system sensor histidine kinase/response regulator, hybrid ('one component system') (NCBI ptt file), at 2,164,759 to 2,167,524
BT1754
Position (kb)
2163
2164
2165 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2162.282 kb on + strand, within BT1751 at 2162.306 kb on - strand, within BT1751 at 2162.440 kb on + strand, within BT1751 at 2162.560 kb on + strand at 2162.561 kb on - strand at 2162.667 kb on - strand at 2162.671 kb on + strand at 2162.683 kb on - strand at 2162.752 kb on + strand at 2162.752 kb on + strand at 2162.839 kb on - strand at 2162.903 kb on + strand at 2162.929 kb on - strand at 2162.947 kb on + strand at 2162.948 kb on - strand at 2163.012 kb on + strand at 2163.117 kb on + strand at 2163.119 kb on + strand at 2163.119 kb on + strand at 2163.254 kb on - strand at 2163.295 kb on - strand at 2163.320 kb on - strand at 2163.339 kb on - strand at 2163.493 kb on + strand, within BT1752 at 2163.677 kb on + strand, within BT1752 at 2163.713 kb on + strand, within BT1752 at 2163.967 kb on - strand, within BT1752 at 2164.139 kb on - strand, within BT1752 at 2164.171 kb on - strand, within BT1752 at 2164.171 kb on - strand, within BT1752 at 2164.173 kb on - strand, within BT1752 at 2164.533 kb on + strand, within BT1753 at 2164.624 kb on - strand, within BT1753 at 2164.624 kb on - strand, within BT1753 at 2164.632 kb on - strand, within BT1753 at 2164.635 kb on + strand, within BT1753 at 2164.636 kb on - strand, within BT1753 at 2164.655 kb on - strand, within BT1753 at 2164.655 kb on - strand, within BT1753 at 2164.720 kb on - strand at 2164.722 kb on - strand at 2164.732 kb on - strand at 2164.732 kb on - strand at 2164.734 kb on - strand at 2164.743 kb on + strand at 2164.809 kb on + strand at 2164.810 kb on - strand at 2164.947 kb on + strand at 2165.242 kb on - strand, within BT1754 at 2165.299 kb on + strand, within BT1754 at 2165.389 kb on - strand, within BT1754 at 2165.434 kb on - strand, within BT1754
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.1 mM remove 2,162,282 + BT1751 0.68 -1.0 2,162,306 - BT1751 0.70 +1.1 2,162,440 + BT1751 0.81 -0.2 2,162,560 + +1.1 2,162,561 - -1.2 2,162,667 - -0.5 2,162,671 + +0.1 2,162,683 - -1.5 2,162,752 + -0.9 2,162,752 + -0.5 2,162,839 - +1.1 2,162,903 + +0.1 2,162,929 - -0.9 2,162,947 + -2.9 2,162,948 - -1.5 2,163,012 + -3.0 2,163,117 + -0.5 2,163,119 + +0.1 2,163,119 + -1.3 2,163,254 - +0.1 2,163,295 - -0.9 2,163,320 - +0.1 2,163,339 - -1.5 2,163,493 + BT1752 0.18 -3.5 2,163,677 + BT1752 0.34 +0.1 2,163,713 + BT1752 0.37 -2.2 2,163,967 - BT1752 0.59 -0.9 2,164,139 - BT1752 0.74 +0.1 2,164,171 - BT1752 0.77 -1.9 2,164,171 - BT1752 0.77 +0.1 2,164,173 - BT1752 0.77 -1.5 2,164,533 + BT1753 0.40 +0.6 2,164,624 - BT1753 0.68 -1.7 2,164,624 - BT1753 0.68 -2.1 2,164,632 - BT1753 0.70 -1.9 2,164,635 + BT1753 0.71 -1.6 2,164,636 - BT1753 0.71 -1.7 2,164,655 - BT1753 0.77 +0.1 2,164,655 - BT1753 0.77 -1.9 2,164,720 - +0.1 2,164,722 - -2.5 2,164,732 - +0.1 2,164,732 - -0.5 2,164,734 - -1.3 2,164,743 + -1.1 2,164,809 + -0.9 2,164,810 - -0.9 2,164,947 + +1.9 2,165,242 - BT1754 0.17 -1.3 2,165,299 + BT1754 0.20 +0.1 2,165,389 - BT1754 0.23 -2.1 2,165,434 - BT1754 0.24 +1.1
Or see this region's nucleotide sequence