Experiment: BHIS with Cephalothin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0960 and BT0961 are separated by 73 nucleotides BT0961 and BT0962 overlap by 1 nucleotides BT0962 and BT0963 are separated by 19 nucleotides
BT0960: BT0960 - putative TonB-dependent outer membrane receptor (NCBI ptt file), at 1,173,791 to 1,175,632
BT0960
BT0961: BT0961 - conserved hypothetical protein (NCBI ptt file), at 1,175,706 to 1,176,140
BT0961
BT0962: BT0962 - aldo/keto reductase (NCBI ptt file), at 1,176,140 to 1,177,543
BT0962
BT0963: BT0963 - putative ferredoxin-type protein (NCBI ptt file), at 1,177,563 to 1,179,071
BT0963
Position (kb) 1176
1177
1178 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1175.183 kb on + strand, within BT0960 at 1175.192 kb on + strand, within BT0960 at 1175.218 kb on - strand, within BT0960 at 1175.274 kb on + strand, within BT0960 at 1175.416 kb on + strand, within BT0960 at 1175.419 kb on + strand, within BT0960 at 1175.510 kb on + strand at 1175.521 kb on + strand at 1175.843 kb on + strand, within BT0961 at 1175.924 kb on + strand, within BT0961 at 1175.979 kb on + strand, within BT0961 at 1176.069 kb on - strand, within BT0961 at 1176.072 kb on + strand, within BT0961 at 1176.072 kb on + strand, within BT0961 at 1176.073 kb on - strand, within BT0961 at 1176.073 kb on - strand, within BT0961 at 1176.117 kb on + strand at 1176.141 kb on + strand at 1176.200 kb on + strand at 1176.203 kb on - strand at 1176.206 kb on + strand at 1176.375 kb on + strand, within BT0962 at 1176.456 kb on + strand, within BT0962 at 1176.529 kb on - strand, within BT0962 at 1176.560 kb on - strand, within BT0962 at 1176.561 kb on + strand, within BT0962 at 1176.587 kb on + strand, within BT0962 at 1176.587 kb on + strand, within BT0962 at 1176.630 kb on - strand, within BT0962 at 1176.739 kb on + strand, within BT0962 at 1176.739 kb on + strand, within BT0962 at 1176.744 kb on - strand, within BT0962 at 1176.817 kb on + strand, within BT0962 at 1176.897 kb on + strand, within BT0962 at 1177.024 kb on + strand, within BT0962 at 1177.026 kb on + strand, within BT0962 at 1177.377 kb on + strand, within BT0962 at 1177.475 kb on - strand at 1177.486 kb on + strand at 1177.872 kb on + strand, within BT0963 at 1177.965 kb on + strand, within BT0963 at 1177.995 kb on + strand, within BT0963 at 1177.996 kb on - strand, within BT0963 at 1178.136 kb on + strand, within BT0963 at 1178.141 kb on + strand, within BT0963 at 1178.141 kb on + strand, within BT0963 at 1178.217 kb on + strand, within BT0963 at 1178.327 kb on + strand, within BT0963 at 1178.369 kb on + strand, within BT0963 at 1178.493 kb on - strand, within BT0963
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.1 mM remove 1,175,183 + BT0960 0.76 +2.0 1,175,192 + BT0960 0.76 +1.2 1,175,218 - BT0960 0.77 -0.0 1,175,274 + BT0960 0.81 -1.6 1,175,416 + BT0960 0.88 -1.0 1,175,419 + BT0960 0.88 -1.4 1,175,510 + +0.5 1,175,521 + -1.2 1,175,843 + BT0961 0.31 -2.2 1,175,924 + BT0961 0.50 -0.0 1,175,979 + BT0961 0.63 -2.0 1,176,069 - BT0961 0.83 -3.5 1,176,072 + BT0961 0.84 -1.0 1,176,072 + BT0961 0.84 -0.0 1,176,073 - BT0961 0.84 -3.6 1,176,073 - BT0961 0.84 -0.2 1,176,117 + -2.4 1,176,141 + +2.5 1,176,200 + -1.0 1,176,203 - -1.6 1,176,206 + -2.0 1,176,375 + BT0962 0.17 -0.0 1,176,456 + BT0962 0.23 -0.3 1,176,529 - BT0962 0.28 -1.0 1,176,560 - BT0962 0.30 -2.9 1,176,561 + BT0962 0.30 -0.3 1,176,587 + BT0962 0.32 -1.0 1,176,587 + BT0962 0.32 +2.3 1,176,630 - BT0962 0.35 +1.4 1,176,739 + BT0962 0.43 -1.0 1,176,739 + BT0962 0.43 -1.6 1,176,744 - BT0962 0.43 -0.0 1,176,817 + BT0962 0.48 +1.5 1,176,897 + BT0962 0.54 +3.1 1,177,024 + BT0962 0.63 +1.5 1,177,026 + BT0962 0.63 +1.3 1,177,377 + BT0962 0.88 -2.9 1,177,475 - -4.0 1,177,486 + -2.4 1,177,872 + BT0963 0.20 -3.2 1,177,965 + BT0963 0.27 -0.0 1,177,995 + BT0963 0.29 -0.0 1,177,996 - BT0963 0.29 -0.0 1,178,136 + BT0963 0.38 +3.5 1,178,141 + BT0963 0.38 -1.5 1,178,141 + BT0963 0.38 +1.5 1,178,217 + BT0963 0.43 +2.0 1,178,327 + BT0963 0.51 -1.6 1,178,369 + BT0963 0.53 -0.0 1,178,493 - BT0963 0.62 -0.6
Or see this region's nucleotide sequence