Experiment: Varel_Bryant_medium_Glucose with Geneticin G418 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1655 and BT1656 are separated by 10 nucleotides BT1656 and BT1657 are separated by 335 nucleotides BT1657 and BT1658 are separated by 172 nucleotides BT1658 and BT1659 are separated by 67 nucleotides
BT1655: BT1655 - conserved hypothetical protein (NCBI ptt file), at 2,047,034 to 2,047,402
BT1655
BT1656: BT1656 - putative transcriptional regulator (NCBI ptt file), at 2,047,413 to 2,047,991
BT1656
BT1657: BT1657 - integrase (NCBI ptt file), at 2,048,327 to 2,049,277
BT1657
BT1658: BT1658 - transaldolase (NCBI ptt file), at 2,049,450 to 2,050,106
BT1658
BT1659: BT1659 - fructose-bisphosphate aldolase class I (NCBI ptt file), at 2,050,174 to 2,051,226
BT1659
Position (kb)
2048
2049
2050 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2047.499 kb on + strand, within BT1656 at 2047.766 kb on + strand, within BT1656 at 2047.766 kb on + strand, within BT1656 at 2048.094 kb on + strand at 2048.156 kb on - strand at 2048.172 kb on + strand at 2048.970 kb on + strand, within BT1657 at 2049.007 kb on + strand, within BT1657 at 2049.090 kb on + strand, within BT1657 at 2049.149 kb on - strand, within BT1657 at 2049.259 kb on + strand at 2049.282 kb on + strand at 2049.357 kb on + strand at 2049.361 kb on - strand at 2049.369 kb on + strand at 2049.369 kb on + strand at 2049.369 kb on + strand at 2049.370 kb on - strand at 2049.383 kb on - strand at 2049.393 kb on + strand at 2049.415 kb on - strand at 2049.417 kb on - strand at 2049.434 kb on + strand at 2049.467 kb on + strand at 2049.615 kb on + strand, within BT1658 at 2049.615 kb on + strand, within BT1658 at 2049.616 kb on - strand, within BT1658 at 2049.647 kb on + strand, within BT1658 at 2049.651 kb on + strand, within BT1658 at 2049.652 kb on - strand, within BT1658 at 2049.652 kb on - strand, within BT1658 at 2049.652 kb on - strand, within BT1658 at 2049.776 kb on + strand, within BT1658 at 2049.809 kb on - strand, within BT1658 at 2049.908 kb on - strand, within BT1658 at 2049.918 kb on + strand, within BT1658 at 2049.919 kb on - strand, within BT1658 at 2049.922 kb on - strand, within BT1658 at 2049.953 kb on + strand, within BT1658 at 2049.954 kb on - strand, within BT1658 at 2049.962 kb on + strand, within BT1658 at 2049.967 kb on + strand, within BT1658 at 2050.044 kb on + strand at 2050.107 kb on - strand at 2050.107 kb on - strand at 2050.131 kb on - strand at 2050.155 kb on - strand at 2050.170 kb on + strand at 2050.203 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Geneticin G418 0.2 mM remove 2,047,499 + BT1656 0.15 -1.8 2,047,766 + BT1656 0.61 +0.1 2,047,766 + BT1656 0.61 +0.1 2,048,094 + -0.7 2,048,156 - -2.9 2,048,172 + -1.0 2,048,970 + BT1657 0.68 -1.7 2,049,007 + BT1657 0.72 -1.6 2,049,090 + BT1657 0.80 -3.4 2,049,149 - BT1657 0.86 +1.5 2,049,259 + +0.3 2,049,282 + -1.0 2,049,357 + +0.6 2,049,361 - +2.9 2,049,369 + +1.5 2,049,369 + +1.7 2,049,369 + -0.3 2,049,370 - -0.9 2,049,383 - +1.1 2,049,393 + +0.1 2,049,415 - +1.7 2,049,417 - +0.1 2,049,434 + -1.4 2,049,467 + -0.9 2,049,615 + BT1658 0.25 +0.7 2,049,615 + BT1658 0.25 +1.1 2,049,616 - BT1658 0.25 -1.6 2,049,647 + BT1658 0.30 -0.6 2,049,651 + BT1658 0.31 +1.7 2,049,652 - BT1658 0.31 +0.1 2,049,652 - BT1658 0.31 -0.1 2,049,652 - BT1658 0.31 +1.7 2,049,776 + BT1658 0.50 -2.5 2,049,809 - BT1658 0.55 -1.2 2,049,908 - BT1658 0.70 -0.5 2,049,918 + BT1658 0.71 -0.9 2,049,919 - BT1658 0.71 -0.4 2,049,922 - BT1658 0.72 -1.5 2,049,953 + BT1658 0.77 -1.2 2,049,954 - BT1658 0.77 +0.1 2,049,962 + BT1658 0.78 -0.8 2,049,967 + BT1658 0.79 -2.1 2,050,044 + +0.1 2,050,107 - -1.5 2,050,107 - -2.5 2,050,131 - -0.5 2,050,155 - +0.9 2,050,170 + -0.5 2,050,203 - -1.2
Or see this region's nucleotide sequence