Experiment: Varel_Bryant_medium_Glucose with Geneticin G418 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1366 and BT1367 are separated by 83 nucleotides BT1367 and BT1368 overlap by 4 nucleotides
BT1366: BT1366 - hypothetical protein (NCBI ptt file), at 1,694,426 to 1,696,003
BT1366
BT1367: BT1367 - hydrolase, metal-dependent (NCBI ptt file), at 1,696,087 to 1,696,845
BT1367
BT1368: BT1368 - UDP-N-acetylenolpyruvoylglucosamine reductase (NCBI ptt file), at 1,696,842 to 1,697,837
BT1368
Position (kb)
1696
1697 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1695.104 kb on - strand, within BT1366 at 1695.241 kb on + strand, within BT1366 at 1695.428 kb on + strand, within BT1366 at 1695.429 kb on - strand, within BT1366 at 1695.429 kb on - strand, within BT1366 at 1695.429 kb on - strand, within BT1366 at 1695.429 kb on - strand, within BT1366 at 1695.429 kb on - strand, within BT1366 at 1695.440 kb on + strand, within BT1366 at 1695.523 kb on + strand, within BT1366 at 1695.523 kb on + strand, within BT1366 at 1695.524 kb on - strand, within BT1366 at 1695.529 kb on + strand, within BT1366 at 1695.542 kb on - strand, within BT1366 at 1695.580 kb on - strand, within BT1366 at 1695.778 kb on - strand, within BT1366 at 1695.877 kb on + strand at 1695.878 kb on - strand at 1695.879 kb on + strand at 1695.880 kb on - strand at 1695.880 kb on - strand at 1695.931 kb on - strand at 1695.992 kb on - strand at 1696.117 kb on - strand at 1696.117 kb on - strand at 1696.148 kb on - strand at 1696.151 kb on + strand at 1696.167 kb on + strand, within BT1367 at 1696.173 kb on - strand, within BT1367 at 1696.191 kb on - strand, within BT1367 at 1696.191 kb on - strand, within BT1367 at 1696.294 kb on + strand, within BT1367 at 1696.295 kb on - strand, within BT1367 at 1696.509 kb on - strand, within BT1367 at 1696.541 kb on + strand, within BT1367 at 1696.557 kb on + strand, within BT1367 at 1696.616 kb on + strand, within BT1367 at 1696.646 kb on - strand, within BT1367 at 1696.699 kb on + strand, within BT1367 at 1696.752 kb on + strand, within BT1367 at 1696.789 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Geneticin G418 0.2 mM remove 1,695,104 - BT1366 0.43 -0.9 1,695,241 + BT1366 0.52 -0.9 1,695,428 + BT1366 0.63 +0.1 1,695,429 - BT1366 0.64 -1.2 1,695,429 - BT1366 0.64 -1.5 1,695,429 - BT1366 0.64 -0.9 1,695,429 - BT1366 0.64 -2.6 1,695,429 - BT1366 0.64 +0.1 1,695,440 + BT1366 0.64 -0.3 1,695,523 + BT1366 0.70 +2.7 1,695,523 + BT1366 0.70 -1.9 1,695,524 - BT1366 0.70 +1.1 1,695,529 + BT1366 0.70 -1.9 1,695,542 - BT1366 0.71 -1.5 1,695,580 - BT1366 0.73 -1.5 1,695,778 - BT1366 0.86 -1.5 1,695,877 + +0.1 1,695,878 - -1.5 1,695,879 + +0.1 1,695,880 - +0.1 1,695,880 - -2.5 1,695,931 - -2.2 1,695,992 - -1.8 1,696,117 - -1.5 1,696,117 - +0.9 1,696,148 - -0.6 1,696,151 + -0.9 1,696,167 + BT1367 0.11 +0.1 1,696,173 - BT1367 0.11 -2.7 1,696,191 - BT1367 0.14 -2.2 1,696,191 - BT1367 0.14 -3.1 1,696,294 + BT1367 0.27 +0.1 1,696,295 - BT1367 0.27 -0.6 1,696,509 - BT1367 0.56 -4.1 1,696,541 + BT1367 0.60 +0.1 1,696,557 + BT1367 0.62 +1.1 1,696,616 + BT1367 0.70 -2.5 1,696,646 - BT1367 0.74 +1.7 1,696,699 + BT1367 0.81 -3.2 1,696,752 + BT1367 0.88 -1.8 1,696,789 - -0.5
Or see this region's nucleotide sequence