Experiment: Varel_Bryant_medium_Glucose with Geneticin G418 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0332 and BT0333 are separated by 12 nucleotides BT0333 and BT0334 are separated by 34 nucleotides BT0334 and BT0335 are separated by 292 nucleotides BT0335 and BT0336 overlap by 11 nucleotides
BT0332: BT0332 - 2-oxoisovalerate oxidoreductase beta subunit (NCBI ptt file), at 397,900 to 398,079
BT0332
BT0333: BT0333 - conserved hypothetical protein (NCBI ptt file), at 398,092 to 398,319
BT0333
BT0334: BT0334 - conserved hypothetical protein (NCBI ptt file), at 398,354 to 398,635
BT0334
BT0335: BT0335 - putative ATPase (NCBI ptt file), at 398,928 to 400,481
BT0335
BT0336: BT0336 - conserved hypothetical protein (NCBI ptt file), at 400,471 to 401,913
BT0336
Position (kb) 398
399
400
401 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 398.525 kb on - strand, within BT0334 at 398.790 kb on - strand at 398.792 kb on - strand at 398.816 kb on - strand at 398.816 kb on - strand at 398.824 kb on + strand at 398.825 kb on - strand at 398.834 kb on + strand at 398.843 kb on - strand at 398.850 kb on + strand at 398.901 kb on + strand at 398.924 kb on + strand at 398.924 kb on + strand at 398.924 kb on + strand at 398.945 kb on + strand at 398.946 kb on - strand at 398.947 kb on + strand at 398.981 kb on - strand at 398.983 kb on - strand at 399.205 kb on - strand, within BT0335 at 399.233 kb on + strand, within BT0335 at 399.584 kb on - strand, within BT0335 at 399.767 kb on - strand, within BT0335 at 399.775 kb on - strand, within BT0335 at 399.877 kb on + strand, within BT0335 at 399.883 kb on + strand, within BT0335 at 400.016 kb on + strand, within BT0335 at 400.020 kb on + strand, within BT0335 at 400.092 kb on + strand, within BT0335 at 400.124 kb on + strand, within BT0335 at 400.127 kb on - strand, within BT0335 at 400.405 kb on - strand at 400.465 kb on - strand at 400.465 kb on - strand at 400.479 kb on + strand at 400.483 kb on + strand at 400.498 kb on + strand at 400.571 kb on - strand at 400.608 kb on - strand at 400.699 kb on - strand, within BT0336 at 400.785 kb on - strand, within BT0336 at 400.787 kb on + strand, within BT0336 at 401.088 kb on + strand, within BT0336 at 401.163 kb on + strand, within BT0336 at 401.226 kb on - strand, within BT0336 at 401.230 kb on - strand, within BT0336 at 401.356 kb on + strand, within BT0336 at 401.388 kb on + strand, within BT0336 at 401.457 kb on - strand, within BT0336
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Geneticin G418 0.2 mM remove 398,525 - BT0334 0.61 -1.0 398,790 - -1.0 398,792 - -1.5 398,816 - +1.6 398,816 - -4.3 398,824 + +0.2 398,825 - -4.3 398,834 + +0.0 398,843 - -1.0 398,850 + -1.2 398,901 + +2.0 398,924 + +0.5 398,924 + +1.6 398,924 + -2.1 398,945 + -0.9 398,946 - +1.6 398,947 + -3.3 398,981 - +2.0 398,983 - -1.5 399,205 - BT0335 0.18 -0.5 399,233 + BT0335 0.20 +2.0 399,584 - BT0335 0.42 +1.0 399,767 - BT0335 0.54 -1.5 399,775 - BT0335 0.55 -1.0 399,877 + BT0335 0.61 +2.4 399,883 + BT0335 0.61 +1.5 400,016 + BT0335 0.70 +0.8 400,020 + BT0335 0.70 -1.4 400,092 + BT0335 0.75 +0.0 400,124 + BT0335 0.77 +0.0 400,127 - BT0335 0.77 +1.3 400,405 - -1.5 400,465 - -0.7 400,465 - +2.2 400,479 + -2.9 400,483 + -1.5 400,498 + -3.8 400,571 - -1.1 400,608 - -1.5 400,699 - BT0336 0.16 -1.7 400,785 - BT0336 0.22 +0.8 400,787 + BT0336 0.22 -0.1 401,088 + BT0336 0.43 -1.5 401,163 + BT0336 0.48 +1.0 401,226 - BT0336 0.52 -1.5 401,230 - BT0336 0.53 -1.3 401,356 + BT0336 0.61 -0.7 401,388 + BT0336 0.64 +0.0 401,457 - BT0336 0.68 -1.9
Or see this region's nucleotide sequence