Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS02220

Experiment: water survival rep C; Treatment=watercosm; Collection=outgrowth_in_CPG; Time=7_days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS02215 and RS_RS02220 are separated by 226 nucleotidesRS_RS02220 and RS_RS02225 are separated by 28 nucleotides RS_RS02215: RS_RS02215 - acetate kinase, at 474,524 to 475,864 _RS02215 RS_RS02220: RS_RS02220 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 476,091 to 476,924 _RS02220 RS_RS02225: RS_RS02225 - threonine ammonia-lyase, biosynthetic, at 476,953 to 478,476 _RS02225 Position (kb) 476 477Strain fitness (log2 ratio) -3 -2 -1 0 1at 475.221 kb on + strand, within RS_RS02215at 475.222 kb on - strand, within RS_RS02215at 475.228 kb on - strand, within RS_RS02215at 475.389 kb on + strand, within RS_RS02215at 475.950 kb on + strandat 475.951 kb on - strandat 475.951 kb on - strandat 475.953 kb on - strandat 476.011 kb on + strandat 476.092 kb on + strandat 476.177 kb on + strand, within RS_RS02220at 476.178 kb on - strand, within RS_RS02220at 476.179 kb on + strand, within RS_RS02220at 476.291 kb on - strand, within RS_RS02220at 476.330 kb on - strand, within RS_RS02220at 476.761 kb on + strand, within RS_RS02220at 476.869 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction water survival rep C; Treatment=watercosm; Collection=outgrowth_in_CPG; Time=7_days
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475,221 + RS_RS02215 0.52 +0.1
475,222 - RS_RS02215 0.52 +0.1
475,228 - RS_RS02215 0.52 +0.6
475,389 + RS_RS02215 0.65 +0.3
475,950 + -0.1
475,951 - -0.0
475,951 - -0.9
475,953 - -0.2
476,011 + +1.0
476,092 + -0.4
476,177 + RS_RS02220 0.10 +0.0
476,178 - RS_RS02220 0.10 -1.3
476,179 + RS_RS02220 0.11 -1.4
476,291 - RS_RS02220 0.24 -1.4
476,330 - RS_RS02220 0.29 +1.0
476,761 + RS_RS02220 0.80 -3.0
476,869 + -0.6

Or see this region's nucleotide sequence