Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4544 and BT4545 are separated by 676 nucleotides BT4545 and BT4546 are separated by 69 nucleotides
BT4544: BT4544 - transposase (NCBI ptt file), at 5,964,600 to 5,965,526
BT4544
BT4545: BT4545 - hypothetical protein (NCBI ptt file), at 5,966,203 to 5,966,448
BT4545
BT4546: BT4546 - conserved hypothetical protein (NCBI ptt file), at 5,966,518 to 5,967,591
BT4546
Position (kb)
5966
5967 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5965.302 kb on + strand, within BT4544 at 5965.303 kb on - strand, within BT4544 at 5965.303 kb on - strand, within BT4544 at 5965.315 kb on + strand, within BT4544 at 5965.316 kb on - strand, within BT4544 at 5965.384 kb on + strand, within BT4544 at 5965.458 kb on + strand at 5965.698 kb on - strand at 5965.793 kb on - strand at 5965.807 kb on + strand at 5965.807 kb on + strand at 5965.808 kb on - strand at 5965.906 kb on - strand at 5965.971 kb on + strand at 5966.178 kb on - strand at 5966.191 kb on - strand at 5966.199 kb on + strand at 5966.200 kb on - strand at 5966.200 kb on - strand at 5966.292 kb on - strand, within BT4545 at 5966.350 kb on + strand, within BT4545 at 5966.351 kb on - strand, within BT4545 at 5966.369 kb on + strand, within BT4545 at 5966.380 kb on + strand, within BT4545 at 5966.396 kb on + strand, within BT4545 at 5966.397 kb on - strand, within BT4545 at 5966.465 kb on + strand at 5966.476 kb on + strand at 5966.614 kb on - strand at 5966.808 kb on - strand, within BT4546 at 5966.846 kb on + strand, within BT4546 at 5966.880 kb on + strand, within BT4546 at 5967.068 kb on - strand, within BT4546 at 5967.069 kb on + strand, within BT4546 at 5967.070 kb on - strand, within BT4546 at 5967.070 kb on - strand, within BT4546 at 5967.070 kb on - strand, within BT4546 at 5967.116 kb on - strand, within BT4546 at 5967.170 kb on - strand, within BT4546 at 5967.175 kb on - strand, within BT4546 at 5967.356 kb on + strand, within BT4546 at 5967.391 kb on + strand, within BT4546 at 5967.397 kb on - strand, within BT4546 at 5967.397 kb on - strand, within BT4546
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 5,965,302 + BT4544 0.76 +0.6 5,965,303 - BT4544 0.76 -1.5 5,965,303 - BT4544 0.76 -2.3 5,965,315 + BT4544 0.77 -1.8 5,965,316 - BT4544 0.77 -2.8 5,965,384 + BT4544 0.85 -0.2 5,965,458 + -0.6 5,965,698 - -1.0 5,965,793 - -2.6 5,965,807 + +0.0 5,965,807 + -2.6 5,965,808 - +0.0 5,965,906 - -0.6 5,965,971 + +0.0 5,966,178 - -0.6 5,966,191 - -1.0 5,966,199 + -2.6 5,966,200 - -3.1 5,966,200 - -1.9 5,966,292 - BT4545 0.36 -1.6 5,966,350 + BT4545 0.60 -3.3 5,966,351 - BT4545 0.60 +0.0 5,966,369 + BT4545 0.67 -2.0 5,966,380 + BT4545 0.72 -3.4 5,966,396 + BT4545 0.78 +0.0 5,966,397 - BT4545 0.79 +1.6 5,966,465 + -2.6 5,966,476 + +1.2 5,966,614 - -2.0 5,966,808 - BT4546 0.27 -2.0 5,966,846 + BT4546 0.31 +0.9 5,966,880 + BT4546 0.34 +0.0 5,967,068 - BT4546 0.51 -3.7 5,967,069 + BT4546 0.51 -2.0 5,967,070 - BT4546 0.51 -2.3 5,967,070 - BT4546 0.51 -0.7 5,967,070 - BT4546 0.51 -1.0 5,967,116 - BT4546 0.56 -1.6 5,967,170 - BT4546 0.61 +0.0 5,967,175 - BT4546 0.61 -0.6 5,967,356 + BT4546 0.78 -1.1 5,967,391 + BT4546 0.81 -0.6 5,967,397 - BT4546 0.82 -1.3 5,967,397 - BT4546 0.82 -1.0
Or see this region's nucleotide sequence