Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2317 and BT2318 are separated by 81 nucleotides BT2318 and BT2319 are separated by 5 nucleotides
BT2317: BT2317 - hypothetical protein (NCBI ptt file), at 2,892,648 to 2,893,808
BT2317
BT2318: BT2318 - hypothetical protein (NCBI ptt file), at 2,893,890 to 2,894,822
BT2318
BT2319: BT2319 - conserved hypothetical protein (NCBI ptt file), at 2,894,828 to 2,896,120
BT2319
Position (kb)
2893
2894
2895 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2892.979 kb on + strand, within BT2317 at 2893.003 kb on + strand, within BT2317 at 2893.004 kb on - strand, within BT2317 at 2893.055 kb on + strand, within BT2317 at 2893.056 kb on - strand, within BT2317 at 2893.135 kb on + strand, within BT2317 at 2893.135 kb on + strand, within BT2317 at 2893.281 kb on + strand, within BT2317 at 2893.281 kb on + strand, within BT2317 at 2893.373 kb on + strand, within BT2317 at 2893.498 kb on + strand, within BT2317 at 2893.520 kb on - strand, within BT2317 at 2893.656 kb on + strand, within BT2317 at 2893.657 kb on - strand, within BT2317 at 2893.657 kb on - strand, within BT2317 at 2893.657 kb on - strand, within BT2317 at 2893.770 kb on - strand at 2893.802 kb on - strand at 2893.826 kb on + strand at 2893.850 kb on + strand at 2893.994 kb on + strand, within BT2318 at 2894.050 kb on + strand, within BT2318 at 2894.054 kb on - strand, within BT2318 at 2894.054 kb on - strand, within BT2318 at 2894.100 kb on + strand, within BT2318 at 2894.159 kb on + strand, within BT2318 at 2894.241 kb on - strand, within BT2318 at 2894.322 kb on + strand, within BT2318 at 2894.402 kb on - strand, within BT2318 at 2894.599 kb on + strand, within BT2318 at 2894.599 kb on + strand, within BT2318 at 2894.600 kb on - strand, within BT2318 at 2894.662 kb on + strand, within BT2318 at 2894.663 kb on - strand, within BT2318 at 2894.727 kb on - strand, within BT2318 at 2894.728 kb on + strand, within BT2318 at 2894.729 kb on - strand at 2894.732 kb on + strand at 2894.775 kb on + strand at 2894.778 kb on + strand at 2894.779 kb on - strand at 2894.940 kb on + strand at 2894.940 kb on + strand at 2894.940 kb on + strand at 2894.940 kb on + strand at 2894.970 kb on + strand, within BT2319 at 2894.970 kb on + strand, within BT2319 at 2895.108 kb on + strand, within BT2319 at 2895.108 kb on + strand, within BT2319 at 2895.135 kb on + strand, within BT2319 at 2895.136 kb on - strand, within BT2319 at 2895.211 kb on + strand, within BT2319 at 2895.234 kb on + strand, within BT2319 at 2895.279 kb on + strand, within BT2319 at 2895.279 kb on + strand, within BT2319 at 2895.279 kb on + strand, within BT2319 at 2895.280 kb on - strand, within BT2319 at 2895.691 kb on + strand, within BT2319
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 2,892,979 + BT2317 0.29 -0.9 2,893,003 + BT2317 0.31 -2.3 2,893,004 - BT2317 0.31 -2.2 2,893,055 + BT2317 0.35 -0.1 2,893,056 - BT2317 0.35 -1.3 2,893,135 + BT2317 0.42 -1.1 2,893,135 + BT2317 0.42 -1.8 2,893,281 + BT2317 0.55 -0.4 2,893,281 + BT2317 0.55 -0.8 2,893,373 + BT2317 0.62 -2.1 2,893,498 + BT2317 0.73 -1.6 2,893,520 - BT2317 0.75 -1.9 2,893,656 + BT2317 0.87 -1.6 2,893,657 - BT2317 0.87 -1.5 2,893,657 - BT2317 0.87 -0.1 2,893,657 - BT2317 0.87 -0.5 2,893,770 - +0.5 2,893,802 - -1.1 2,893,826 + -2.9 2,893,850 + -1.1 2,893,994 + BT2318 0.11 -0.8 2,894,050 + BT2318 0.17 -2.1 2,894,054 - BT2318 0.18 -1.2 2,894,054 - BT2318 0.18 -3.4 2,894,100 + BT2318 0.23 -2.4 2,894,159 + BT2318 0.29 -0.1 2,894,241 - BT2318 0.38 -1.9 2,894,322 + BT2318 0.46 -1.1 2,894,402 - BT2318 0.55 -0.1 2,894,599 + BT2318 0.76 -2.6 2,894,599 + BT2318 0.76 -3.8 2,894,600 - BT2318 0.76 -1.1 2,894,662 + BT2318 0.83 -1.6 2,894,663 - BT2318 0.83 -0.1 2,894,727 - BT2318 0.90 -2.8 2,894,728 + BT2318 0.90 -2.4 2,894,729 - +0.9 2,894,732 + -1.9 2,894,775 + -2.1 2,894,778 + -0.1 2,894,779 - -2.2 2,894,940 + -0.1 2,894,940 + -0.9 2,894,940 + -0.7 2,894,940 + -2.4 2,894,970 + BT2319 0.11 -0.5 2,894,970 + BT2319 0.11 -1.6 2,895,108 + BT2319 0.22 -3.1 2,895,108 + BT2319 0.22 -0.4 2,895,135 + BT2319 0.24 -0.9 2,895,136 - BT2319 0.24 -2.4 2,895,211 + BT2319 0.30 -0.7 2,895,234 + BT2319 0.31 -2.3 2,895,279 + BT2319 0.35 -1.3 2,895,279 + BT2319 0.35 -0.5 2,895,279 + BT2319 0.35 -0.1 2,895,280 - BT2319 0.35 -2.4 2,895,691 + BT2319 0.67 -0.8
Or see this region's nucleotide sequence