Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1772 and BT1773 are separated by 21 nucleotides BT1773 and BT1774 are separated by 11 nucleotides
BT1772: BT1772 - conserved hypothetical protein (NCBI ptt file), at 2,200,884 to 2,202,164
BT1772
BT1773: BT1773 - hypothetical protein (NCBI ptt file), at 2,202,186 to 2,204,192
BT1773
BT1774: BT1774 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 2,204,204 to 2,207,509
BT1774
Position (kb)
2202
2203
2204
2205 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2201.198 kb on + strand, within BT1772 at 2201.258 kb on - strand, within BT1772 at 2201.261 kb on + strand, within BT1772 at 2201.306 kb on + strand, within BT1772 at 2201.307 kb on - strand, within BT1772 at 2201.353 kb on + strand, within BT1772 at 2201.414 kb on + strand, within BT1772 at 2201.414 kb on + strand, within BT1772 at 2201.415 kb on - strand, within BT1772 at 2201.444 kb on - strand, within BT1772 at 2201.498 kb on - strand, within BT1772 at 2201.598 kb on + strand, within BT1772 at 2201.615 kb on + strand, within BT1772 at 2201.616 kb on - strand, within BT1772 at 2201.633 kb on + strand, within BT1772 at 2201.639 kb on + strand, within BT1772 at 2201.645 kb on + strand, within BT1772 at 2201.710 kb on + strand, within BT1772 at 2201.710 kb on + strand, within BT1772 at 2201.729 kb on + strand, within BT1772 at 2201.733 kb on + strand, within BT1772 at 2201.753 kb on + strand, within BT1772 at 2201.754 kb on - strand, within BT1772 at 2201.805 kb on - strand, within BT1772 at 2201.975 kb on + strand, within BT1772 at 2202.180 kb on + strand at 2202.185 kb on - strand at 2202.200 kb on + strand at 2202.235 kb on + strand at 2202.395 kb on + strand, within BT1773 at 2202.488 kb on + strand, within BT1773 at 2202.500 kb on - strand, within BT1773 at 2202.562 kb on + strand, within BT1773 at 2202.562 kb on + strand, within BT1773 at 2202.567 kb on + strand, within BT1773 at 2202.762 kb on - strand, within BT1773 at 2202.776 kb on + strand, within BT1773 at 2202.777 kb on - strand, within BT1773 at 2202.812 kb on + strand, within BT1773 at 2202.815 kb on - strand, within BT1773 at 2202.877 kb on + strand, within BT1773 at 2202.877 kb on + strand, within BT1773 at 2202.982 kb on + strand, within BT1773 at 2202.982 kb on + strand, within BT1773 at 2202.986 kb on - strand, within BT1773 at 2203.000 kb on + strand, within BT1773 at 2203.000 kb on + strand, within BT1773 at 2203.000 kb on + strand, within BT1773 at 2203.050 kb on - strand, within BT1773 at 2203.097 kb on + strand, within BT1773 at 2203.136 kb on + strand, within BT1773 at 2203.179 kb on + strand, within BT1773 at 2203.179 kb on + strand, within BT1773 at 2203.265 kb on + strand, within BT1773 at 2203.312 kb on + strand, within BT1773 at 2203.336 kb on + strand, within BT1773 at 2203.349 kb on - strand, within BT1773 at 2203.465 kb on + strand, within BT1773 at 2203.559 kb on + strand, within BT1773 at 2203.590 kb on + strand, within BT1773 at 2203.595 kb on + strand, within BT1773 at 2203.595 kb on + strand, within BT1773 at 2203.662 kb on - strand, within BT1773 at 2203.714 kb on + strand, within BT1773 at 2203.717 kb on + strand, within BT1773 at 2203.717 kb on + strand, within BT1773 at 2203.717 kb on + strand, within BT1773 at 2203.882 kb on + strand, within BT1773 at 2203.920 kb on + strand, within BT1773 at 2203.925 kb on - strand, within BT1773 at 2203.985 kb on + strand, within BT1773 at 2204.082 kb on + strand at 2204.090 kb on - strand at 2204.132 kb on + strand at 2204.164 kb on - strand at 2204.173 kb on - strand at 2204.336 kb on - strand at 2204.336 kb on - strand at 2204.482 kb on + strand at 2204.484 kb on + strand at 2204.514 kb on + strand at 2204.522 kb on + strand at 2204.783 kb on - strand, within BT1774 at 2204.806 kb on + strand, within BT1774 at 2204.935 kb on - strand, within BT1774 at 2204.935 kb on - strand, within BT1774 at 2204.935 kb on - strand, within BT1774 at 2205.015 kb on + strand, within BT1774
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 2,201,198 + BT1772 0.25 -2.6 2,201,258 - BT1772 0.29 -1.2 2,201,261 + BT1772 0.29 +0.1 2,201,306 + BT1772 0.33 +0.1 2,201,307 - BT1772 0.33 +0.1 2,201,353 + BT1772 0.37 +0.1 2,201,414 + BT1772 0.41 +0.1 2,201,414 + BT1772 0.41 +1.1 2,201,415 - BT1772 0.41 +0.1 2,201,444 - BT1772 0.44 -1.5 2,201,498 - BT1772 0.48 -0.9 2,201,598 + BT1772 0.56 -1.1 2,201,615 + BT1772 0.57 -2.0 2,201,616 - BT1772 0.57 -1.1 2,201,633 + BT1772 0.58 -0.7 2,201,639 + BT1772 0.59 +1.1 2,201,645 + BT1772 0.59 +1.7 2,201,710 + BT1772 0.64 -1.1 2,201,710 + BT1772 0.64 -0.8 2,201,729 + BT1772 0.66 +0.2 2,201,733 + BT1772 0.66 -2.1 2,201,753 + BT1772 0.68 -1.8 2,201,754 - BT1772 0.68 -0.2 2,201,805 - BT1772 0.72 -0.2 2,201,975 + BT1772 0.85 -1.9 2,202,180 + +1.9 2,202,185 - -2.2 2,202,200 + +0.1 2,202,235 + -2.7 2,202,395 + BT1773 0.10 +0.1 2,202,488 + BT1773 0.15 -0.7 2,202,500 - BT1773 0.16 +0.7 2,202,562 + BT1773 0.19 -0.9 2,202,562 + BT1773 0.19 -1.9 2,202,567 + BT1773 0.19 -0.4 2,202,762 - BT1773 0.29 +0.1 2,202,776 + BT1773 0.29 +0.8 2,202,777 - BT1773 0.29 -1.8 2,202,812 + BT1773 0.31 -1.5 2,202,815 - BT1773 0.31 -1.0 2,202,877 + BT1773 0.34 -0.4 2,202,877 + BT1773 0.34 -1.5 2,202,982 + BT1773 0.40 -1.5 2,202,982 + BT1773 0.40 +0.1 2,202,986 - BT1773 0.40 -1.9 2,203,000 + BT1773 0.41 -0.6 2,203,000 + BT1773 0.41 -1.1 2,203,000 + BT1773 0.41 +0.1 2,203,050 - BT1773 0.43 -2.2 2,203,097 + BT1773 0.45 -0.7 2,203,136 + BT1773 0.47 +0.1 2,203,179 + BT1773 0.49 -3.5 2,203,179 + BT1773 0.49 -0.3 2,203,265 + BT1773 0.54 -1.5 2,203,312 + BT1773 0.56 -1.9 2,203,336 + BT1773 0.57 -0.7 2,203,349 - BT1773 0.58 -0.9 2,203,465 + BT1773 0.64 -0.5 2,203,559 + BT1773 0.68 -0.8 2,203,590 + BT1773 0.70 -0.0 2,203,595 + BT1773 0.70 -2.9 2,203,595 + BT1773 0.70 -1.2 2,203,662 - BT1773 0.74 -0.7 2,203,714 + BT1773 0.76 +0.1 2,203,717 + BT1773 0.76 -0.7 2,203,717 + BT1773 0.76 +1.7 2,203,717 + BT1773 0.76 -0.9 2,203,882 + BT1773 0.85 +0.1 2,203,920 + BT1773 0.86 -0.3 2,203,925 - BT1773 0.87 +1.1 2,203,985 + BT1773 0.90 -1.2 2,204,082 + -1.3 2,204,090 - -1.5 2,204,132 + -2.7 2,204,164 - -0.5 2,204,173 - -1.1 2,204,336 - +0.1 2,204,336 - -2.7 2,204,482 + -1.3 2,204,484 + -1.5 2,204,514 + +0.1 2,204,522 + -1.5 2,204,783 - BT1774 0.18 +0.1 2,204,806 + BT1774 0.18 -3.1 2,204,935 - BT1774 0.22 -0.9 2,204,935 - BT1774 0.22 -2.5 2,204,935 - BT1774 0.22 +0.1 2,205,015 + BT1774 0.25 -1.5
Or see this region's nucleotide sequence