Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0103 and BT0104 overlap by 4 nucleotides BT0104 and BT0105 overlap by 4 nucleotides BT0105 and BT0106 are separated by 105 nucleotides
BT0103: BT0103 - conserved hypothetical protein (NCBI ptt file), at 95,169 to 96,704
BT0103
BT0104: BT0104 - hypothetical protein (NCBI ptt file), at 96,701 to 96,889
BT0104
BT0105: BT0105 - DNA topoisomerase I (Omega-protein) (NCBI ptt file), at 96,886 to 98,967
BT0105
BT0106: BT0106 - conserved hypothetical protein (NCBI ptt file), at 99,073 to 99,519
BT0106
Position (kb) 96
97
98
99 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 96.140 kb on - strand, within BT0103 at 96.332 kb on - strand, within BT0103 at 96.332 kb on - strand, within BT0103 at 96.337 kb on + strand, within BT0103 at 96.417 kb on + strand, within BT0103 at 96.418 kb on - strand, within BT0103 at 96.426 kb on + strand, within BT0103 at 96.429 kb on - strand, within BT0103 at 96.830 kb on + strand, within BT0104 at 96.883 kb on - strand at 97.075 kb on - strand at 97.161 kb on - strand, within BT0105 at 97.161 kb on - strand, within BT0105 at 97.199 kb on + strand, within BT0105 at 97.406 kb on + strand, within BT0105 at 97.422 kb on + strand, within BT0105 at 97.423 kb on - strand, within BT0105 at 97.499 kb on + strand, within BT0105 at 97.500 kb on - strand, within BT0105 at 97.500 kb on - strand, within BT0105 at 97.543 kb on + strand, within BT0105 at 97.817 kb on + strand, within BT0105 at 97.817 kb on + strand, within BT0105 at 97.848 kb on + strand, within BT0105 at 97.903 kb on + strand, within BT0105 at 97.904 kb on - strand, within BT0105 at 98.276 kb on + strand, within BT0105 at 98.349 kb on + strand, within BT0105 at 98.452 kb on + strand, within BT0105 at 98.453 kb on - strand, within BT0105 at 98.499 kb on + strand, within BT0105 at 98.565 kb on + strand, within BT0105 at 98.630 kb on - strand, within BT0105 at 98.852 kb on + strand at 98.925 kb on + strand at 99.011 kb on - strand at 99.084 kb on + strand at 99.084 kb on + strand at 99.333 kb on + strand, within BT0106 at 99.374 kb on + strand, within BT0106 at 99.399 kb on + strand, within BT0106 at 99.407 kb on + strand, within BT0106 at 99.443 kb on + strand, within BT0106 at 99.482 kb on + strand at 99.482 kb on + strand at 99.574 kb on - strand at 99.574 kb on - strand at 99.584 kb on - strand at 99.616 kb on + strand at 99.622 kb on + strand at 99.638 kb on - strand at 99.666 kb on - strand at 99.723 kb on - strand at 99.731 kb on - strand at 99.731 kb on - strand at 99.808 kb on - strand at 99.812 kb on + strand at 99.813 kb on - strand at 99.817 kb on - strand at 99.841 kb on + strand at 99.841 kb on + strand at 99.842 kb on - strand at 99.842 kb on - strand at 99.857 kb on + strand at 99.877 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 96,140 - BT0103 0.63 -1.2 96,332 - BT0103 0.76 -1.4 96,332 - BT0103 0.76 -1.8 96,337 + BT0103 0.76 -1.0 96,417 + BT0103 0.81 -0.9 96,418 - BT0103 0.81 +0.7 96,426 + BT0103 0.82 -0.9 96,429 - BT0103 0.82 +1.1 96,830 + BT0104 0.68 -1.4 96,883 - -1.1 97,075 - +1.9 97,161 - BT0105 0.13 -0.4 97,161 - BT0105 0.13 +0.1 97,199 + BT0105 0.15 -2.5 97,406 + BT0105 0.25 +0.1 97,422 + BT0105 0.26 -1.1 97,423 - BT0105 0.26 +0.1 97,499 + BT0105 0.29 +0.8 97,500 - BT0105 0.29 -0.5 97,500 - BT0105 0.29 +0.1 97,543 + BT0105 0.32 +0.1 97,817 + BT0105 0.45 +0.1 97,817 + BT0105 0.45 -0.0 97,848 + BT0105 0.46 +0.1 97,903 + BT0105 0.49 -0.9 97,904 - BT0105 0.49 +0.5 98,276 + BT0105 0.67 -1.9 98,349 + BT0105 0.70 -0.5 98,452 + BT0105 0.75 +1.2 98,453 - BT0105 0.75 +0.1 98,499 + BT0105 0.77 -1.5 98,565 + BT0105 0.81 -3.1 98,630 - BT0105 0.84 -2.7 98,852 + -1.1 98,925 + +0.1 99,011 - -1.2 99,084 + -3.2 99,084 + +0.1 99,333 + BT0106 0.58 -0.2 99,374 + BT0106 0.67 -1.2 99,399 + BT0106 0.73 -3.2 99,407 + BT0106 0.75 -0.3 99,443 + BT0106 0.83 +1.9 99,482 + +0.1 99,482 + -3.1 99,574 - +1.7 99,574 - +0.4 99,584 - +0.1 99,616 + -2.0 99,622 + +0.3 99,638 - +0.1 99,666 - -1.2 99,723 - +0.1 99,731 - -0.9 99,731 - -0.1 99,808 - -0.9 99,812 + -0.6 99,813 - -1.9 99,817 - -1.9 99,841 + -0.9 99,841 + -1.5 99,842 - +0.1 99,842 - -2.2 99,857 + -1.5 99,877 - -0.1
Or see this region's nucleotide sequence