Experiment: Pectin 10 g/L (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_004914 and ABIE53_004915 are separated by 201 nucleotides ABIE53_004915 and ABIE53_004916 are separated by 108 nucleotides ABIE53_004916 and ABIE53_004917 are separated by 307 nucleotides
ABIE53_004914: ABIE53_004914 - methyl-accepting chemotaxis protein, at 1,131,609 to 1,133,168
_004914
ABIE53_004915: ABIE53_004915 - hypothetical protein, at 1,133,370 to 1,133,777
_004915
ABIE53_004916: ABIE53_004916 - CspA family cold shock protein, at 1,133,886 to 1,134,089
_004916
ABIE53_004917: ABIE53_004917 - threonine/homoserine/homoserine lactone efflux protein, at 1,134,397 to 1,135,032
_004917
Position (kb)
1133
1134 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1132.430 kb on + strand, within ABIE53_004914 at 1132.430 kb on + strand, within ABIE53_004914 at 1132.431 kb on - strand, within ABIE53_004914 at 1132.431 kb on - strand, within ABIE53_004914 at 1132.578 kb on + strand, within ABIE53_004914 at 1132.616 kb on - strand, within ABIE53_004914 at 1132.640 kb on + strand, within ABIE53_004914 at 1132.640 kb on + strand, within ABIE53_004914 at 1132.640 kb on + strand, within ABIE53_004914 at 1132.640 kb on + strand, within ABIE53_004914 at 1132.640 kb on + strand, within ABIE53_004914 at 1132.640 kb on + strand, within ABIE53_004914 at 1132.640 kb on + strand, within ABIE53_004914 at 1132.641 kb on - strand, within ABIE53_004914 at 1132.641 kb on - strand, within ABIE53_004914 at 1132.641 kb on - strand, within ABIE53_004914 at 1132.641 kb on - strand, within ABIE53_004914 at 1132.641 kb on - strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.642 kb on + strand, within ABIE53_004914 at 1132.643 kb on - strand, within ABIE53_004914 at 1132.643 kb on - strand, within ABIE53_004914 at 1132.643 kb on - strand, within ABIE53_004914 at 1132.643 kb on - strand, within ABIE53_004914 at 1132.643 kb on - strand, within ABIE53_004914 at 1132.643 kb on - strand, within ABIE53_004914 at 1132.691 kb on - strand, within ABIE53_004914 at 1132.739 kb on - strand, within ABIE53_004914 at 1132.739 kb on - strand, within ABIE53_004914 at 1132.793 kb on - strand, within ABIE53_004914 at 1132.793 kb on - strand, within ABIE53_004914 at 1132.793 kb on - strand, within ABIE53_004914 at 1132.872 kb on + strand, within ABIE53_004914 at 1132.900 kb on + strand, within ABIE53_004914 at 1132.900 kb on + strand, within ABIE53_004914 at 1132.901 kb on - strand, within ABIE53_004914 at 1132.901 kb on - strand, within ABIE53_004914 at 1132.901 kb on - strand, within ABIE53_004914 at 1132.915 kb on + strand, within ABIE53_004914 at 1132.996 kb on + strand, within ABIE53_004914 at 1132.996 kb on + strand, within ABIE53_004914 at 1133.069 kb on + strand at 1133.070 kb on - strand at 1133.151 kb on + strand at 1133.152 kb on - strand at 1133.152 kb on - strand at 1133.152 kb on - strand at 1133.170 kb on + strand at 1133.170 kb on + strand at 1133.171 kb on - strand at 1133.201 kb on + strand at 1133.202 kb on - strand at 1133.227 kb on + strand at 1133.227 kb on + strand at 1133.228 kb on - strand at 1133.260 kb on + strand at 1133.274 kb on + strand at 1133.275 kb on - strand at 1133.322 kb on + strand at 1133.323 kb on - strand at 1133.332 kb on + strand at 1133.333 kb on - strand at 1133.333 kb on - strand at 1133.333 kb on - strand at 1133.333 kb on - strand at 1133.360 kb on + strand at 1133.361 kb on - strand at 1133.382 kb on - strand at 1133.443 kb on + strand, within ABIE53_004915 at 1133.444 kb on - strand, within ABIE53_004915 at 1133.444 kb on - strand, within ABIE53_004915 at 1133.455 kb on + strand, within ABIE53_004915 at 1133.455 kb on + strand, within ABIE53_004915 at 1133.455 kb on + strand, within ABIE53_004915 at 1133.455 kb on + strand, within ABIE53_004915 at 1133.456 kb on - strand, within ABIE53_004915 at 1133.456 kb on - strand, within ABIE53_004915 at 1133.464 kb on + strand, within ABIE53_004915 at 1133.464 kb on + strand, within ABIE53_004915 at 1133.464 kb on + strand, within ABIE53_004915 at 1133.464 kb on + strand, within ABIE53_004915 at 1133.465 kb on - strand, within ABIE53_004915 at 1133.569 kb on + strand, within ABIE53_004915 at 1133.569 kb on + strand, within ABIE53_004915 at 1133.569 kb on + strand, within ABIE53_004915 at 1133.569 kb on + strand, within ABIE53_004915 at 1133.569 kb on + strand, within ABIE53_004915 at 1133.569 kb on + strand, within ABIE53_004915 at 1133.569 kb on + strand, within ABIE53_004915 at 1133.570 kb on - strand, within ABIE53_004915 at 1133.570 kb on - strand, within ABIE53_004915 at 1133.570 kb on - strand, within ABIE53_004915 at 1133.570 kb on - strand, within ABIE53_004915 at 1133.577 kb on + strand, within ABIE53_004915 at 1133.579 kb on + strand, within ABIE53_004915 at 1133.579 kb on + strand, within ABIE53_004915 at 1133.579 kb on + strand, within ABIE53_004915 at 1133.579 kb on + strand, within ABIE53_004915 at 1133.579 kb on + strand, within ABIE53_004915 at 1133.579 kb on + strand, within ABIE53_004915 at 1133.579 kb on + strand, within ABIE53_004915 at 1133.579 kb on + strand, within ABIE53_004915 at 1133.579 kb on + strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.580 kb on - strand, within ABIE53_004915 at 1133.599 kb on - strand, within ABIE53_004915 at 1133.603 kb on + strand, within ABIE53_004915 at 1133.603 kb on + strand, within ABIE53_004915 at 1133.604 kb on - strand, within ABIE53_004915 at 1133.604 kb on - strand, within ABIE53_004915 at 1133.606 kb on + strand, within ABIE53_004915 at 1133.606 kb on + strand, within ABIE53_004915 at 1133.606 kb on + strand, within ABIE53_004915 at 1133.607 kb on - strand, within ABIE53_004915 at 1133.607 kb on - strand, within ABIE53_004915 at 1133.611 kb on + strand, within ABIE53_004915 at 1133.611 kb on + strand, within ABIE53_004915 at 1133.611 kb on + strand, within ABIE53_004915 at 1133.611 kb on + strand, within ABIE53_004915 at 1133.611 kb on + strand, within ABIE53_004915 at 1133.612 kb on - strand, within ABIE53_004915 at 1133.706 kb on + strand, within ABIE53_004915 at 1133.707 kb on - strand, within ABIE53_004915 at 1133.707 kb on - strand, within ABIE53_004915 at 1133.707 kb on - strand, within ABIE53_004915 at 1133.707 kb on - strand, within ABIE53_004915 at 1133.707 kb on - strand, within ABIE53_004915 at 1133.707 kb on - strand, within ABIE53_004915 at 1133.777 kb on + strand at 1133.777 kb on + strand at 1133.777 kb on + strand at 1133.778 kb on - strand at 1133.778 kb on - strand at 1133.778 kb on - strand at 1133.778 kb on - strand at 1133.778 kb on - strand at 1133.778 kb on - strand at 1133.778 kb on - strand at 1133.887 kb on + strand at 1133.888 kb on - strand at 1133.888 kb on - strand at 1134.071 kb on + strand at 1134.071 kb on + strand at 1134.071 kb on + strand at 1134.071 kb on + strand at 1134.072 kb on - strand at 1134.072 kb on - strand at 1134.119 kb on + strand at 1134.120 kb on - strand at 1134.120 kb on - strand at 1134.120 kb on - strand at 1134.120 kb on - strand at 1134.120 kb on - strand at 1134.120 kb on - strand at 1134.250 kb on - strand at 1134.250 kb on - strand at 1134.265 kb on + strand at 1134.265 kb on + strand at 1134.265 kb on + strand at 1134.265 kb on + strand at 1134.265 kb on + strand at 1134.265 kb on + strand at 1134.265 kb on + strand at 1134.266 kb on - strand at 1134.272 kb on + strand at 1134.272 kb on + strand at 1134.272 kb on + strand at 1134.272 kb on + strand at 1134.273 kb on - strand at 1134.273 kb on - strand at 1134.273 kb on - strand at 1134.273 kb on - strand at 1134.276 kb on + strand at 1134.276 kb on + strand at 1134.276 kb on + strand at 1134.277 kb on - strand at 1134.277 kb on - strand at 1134.277 kb on - strand at 1134.277 kb on - strand at 1134.333 kb on - strand at 1134.494 kb on + strand, within ABIE53_004917 at 1134.494 kb on + strand, within ABIE53_004917 at 1134.494 kb on + strand, within ABIE53_004917 at 1134.495 kb on - strand, within ABIE53_004917 at 1134.495 kb on - strand, within ABIE53_004917 at 1134.542 kb on + strand, within ABIE53_004917 at 1134.542 kb on + strand, within ABIE53_004917 at 1134.543 kb on - strand, within ABIE53_004917 at 1134.543 kb on - strand, within ABIE53_004917 at 1134.543 kb on - strand, within ABIE53_004917 at 1134.543 kb on - strand, within ABIE53_004917 at 1134.587 kb on + strand, within ABIE53_004917 at 1134.587 kb on + strand, within ABIE53_004917 at 1134.587 kb on + strand, within ABIE53_004917 at 1134.587 kb on + strand, within ABIE53_004917 at 1134.588 kb on - strand, within ABIE53_004917 at 1134.588 kb on - strand, within ABIE53_004917 at 1134.588 kb on - strand, within ABIE53_004917 at 1134.630 kb on + strand, within ABIE53_004917 at 1134.638 kb on + strand, within ABIE53_004917 at 1134.641 kb on + strand, within ABIE53_004917 at 1134.642 kb on - strand, within ABIE53_004917 at 1134.776 kb on + strand, within ABIE53_004917 at 1134.776 kb on + strand, within ABIE53_004917 at 1134.776 kb on + strand, within ABIE53_004917 at 1134.776 kb on + strand, within ABIE53_004917 at 1134.776 kb on + strand, within ABIE53_004917 at 1134.777 kb on - strand, within ABIE53_004917 at 1134.777 kb on - strand, within ABIE53_004917 at 1134.777 kb on - strand, within ABIE53_004917 at 1134.777 kb on - strand, within ABIE53_004917 at 1134.777 kb on - strand, within ABIE53_004917
Per-strain Table
Position Strand Gene LocusTag Fraction Pectin 10 g/L (C) remove 1,132,430 + ABIE53_004914 0.53 +0.5 1,132,430 + ABIE53_004914 0.53 -1.2 1,132,431 - ABIE53_004914 0.53 -2.0 1,132,431 - ABIE53_004914 0.53 -0.8 1,132,578 + ABIE53_004914 0.62 -0.1 1,132,616 - ABIE53_004914 0.65 -1.1 1,132,640 + ABIE53_004914 0.66 -1.4 1,132,640 + ABIE53_004914 0.66 +0.9 1,132,640 + ABIE53_004914 0.66 -0.5 1,132,640 + ABIE53_004914 0.66 +0.2 1,132,640 + ABIE53_004914 0.66 -1.1 1,132,640 + ABIE53_004914 0.66 -1.5 1,132,640 + ABIE53_004914 0.66 -1.7 1,132,641 - ABIE53_004914 0.66 -1.9 1,132,641 - ABIE53_004914 0.66 -0.7 1,132,641 - ABIE53_004914 0.66 -0.4 1,132,641 - ABIE53_004914 0.66 -2.4 1,132,641 - ABIE53_004914 0.66 -2.1 1,132,642 + ABIE53_004914 0.66 +0.3 1,132,642 + ABIE53_004914 0.66 -1.1 1,132,642 + ABIE53_004914 0.66 -0.4 1,132,642 + ABIE53_004914 0.66 +1.9 1,132,642 + ABIE53_004914 0.66 -0.7 1,132,642 + ABIE53_004914 0.66 +1.9 1,132,642 + ABIE53_004914 0.66 -0.5 1,132,642 + ABIE53_004914 0.66 -2.2 1,132,642 + ABIE53_004914 0.66 -0.4 1,132,642 + ABIE53_004914 0.66 +0.9 1,132,642 + ABIE53_004914 0.66 +0.9 1,132,642 + ABIE53_004914 0.66 +0.9 1,132,642 + ABIE53_004914 0.66 -1.4 1,132,642 + ABIE53_004914 0.66 -1.2 1,132,642 + ABIE53_004914 0.66 -1.7 1,132,643 - ABIE53_004914 0.66 +1.5 1,132,643 - ABIE53_004914 0.66 +1.1 1,132,643 - ABIE53_004914 0.66 -0.3 1,132,643 - ABIE53_004914 0.66 +0.9 1,132,643 - ABIE53_004914 0.66 -0.1 1,132,643 - ABIE53_004914 0.66 -0.7 1,132,691 - ABIE53_004914 0.69 -0.4 1,132,739 - ABIE53_004914 0.72 +1.5 1,132,739 - ABIE53_004914 0.72 -0.7 1,132,793 - ABIE53_004914 0.76 -0.8 1,132,793 - ABIE53_004914 0.76 +1.2 1,132,793 - ABIE53_004914 0.76 -0.6 1,132,872 + ABIE53_004914 0.81 -0.4 1,132,900 + ABIE53_004914 0.83 +1.3 1,132,900 + ABIE53_004914 0.83 +0.3 1,132,901 - ABIE53_004914 0.83 -0.3 1,132,901 - ABIE53_004914 0.83 -1.3 1,132,901 - ABIE53_004914 0.83 -0.0 1,132,915 + ABIE53_004914 0.84 -2.2 1,132,996 + ABIE53_004914 0.89 +0.5 1,132,996 + ABIE53_004914 0.89 +0.9 1,133,069 + -0.1 1,133,070 - +1.0 1,133,151 + +0.9 1,133,152 - -0.1 1,133,152 - +2.2 1,133,152 - -1.7 1,133,170 + +0.9 1,133,170 + -2.5 1,133,171 - -0.4 1,133,201 + +0.7 1,133,202 - -0.9 1,133,227 + +1.3 1,133,227 + +1.1 1,133,228 - +0.3 1,133,260 + +1.5 1,133,274 + -2.4 1,133,275 - -0.0 1,133,322 + -0.1 1,133,323 - +0.9 1,133,332 + -0.5 1,133,333 - +0.9 1,133,333 - -0.6 1,133,333 - -0.2 1,133,333 - -0.1 1,133,360 + -0.1 1,133,361 - -0.4 1,133,382 - -2.4 1,133,443 + ABIE53_004915 0.18 -2.3 1,133,444 - ABIE53_004915 0.18 +0.9 1,133,444 - ABIE53_004915 0.18 +0.9 1,133,455 + ABIE53_004915 0.21 +0.9 1,133,455 + ABIE53_004915 0.21 -0.3 1,133,455 + ABIE53_004915 0.21 -0.7 1,133,455 + ABIE53_004915 0.21 +0.0 1,133,456 - ABIE53_004915 0.21 -2.7 1,133,456 - ABIE53_004915 0.21 +2.6 1,133,464 + ABIE53_004915 0.23 +3.2 1,133,464 + ABIE53_004915 0.23 -0.7 1,133,464 + ABIE53_004915 0.23 -2.3 1,133,464 + ABIE53_004915 0.23 -0.1 1,133,465 - ABIE53_004915 0.23 +0.2 1,133,569 + ABIE53_004915 0.49 -2.5 1,133,569 + ABIE53_004915 0.49 -0.4 1,133,569 + ABIE53_004915 0.49 -0.7 1,133,569 + ABIE53_004915 0.49 +1.5 1,133,569 + ABIE53_004915 0.49 -2.4 1,133,569 + ABIE53_004915 0.49 -1.0 1,133,569 + ABIE53_004915 0.49 -0.9 1,133,570 - ABIE53_004915 0.49 +1.2 1,133,570 - ABIE53_004915 0.49 -1.1 1,133,570 - ABIE53_004915 0.49 +0.0 1,133,570 - ABIE53_004915 0.49 +0.9 1,133,577 + ABIE53_004915 0.51 -0.9 1,133,579 + ABIE53_004915 0.51 -2.1 1,133,579 + ABIE53_004915 0.51 +0.3 1,133,579 + ABIE53_004915 0.51 -0.7 1,133,579 + ABIE53_004915 0.51 -0.9 1,133,579 + ABIE53_004915 0.51 +0.1 1,133,579 + ABIE53_004915 0.51 -0.7 1,133,579 + ABIE53_004915 0.51 -0.1 1,133,579 + ABIE53_004915 0.51 +1.5 1,133,579 + ABIE53_004915 0.51 -0.1 1,133,580 - ABIE53_004915 0.51 -0.1 1,133,580 - ABIE53_004915 0.51 -1.2 1,133,580 - ABIE53_004915 0.51 -0.1 1,133,580 - ABIE53_004915 0.51 -0.5 1,133,580 - ABIE53_004915 0.51 -0.9 1,133,580 - ABIE53_004915 0.51 -1.6 1,133,580 - ABIE53_004915 0.51 +1.9 1,133,580 - ABIE53_004915 0.51 -2.9 1,133,580 - ABIE53_004915 0.51 -0.1 1,133,580 - ABIE53_004915 0.51 +0.9 1,133,580 - ABIE53_004915 0.51 -1.4 1,133,580 - ABIE53_004915 0.51 -0.4 1,133,580 - ABIE53_004915 0.51 +0.1 1,133,599 - ABIE53_004915 0.56 -0.2 1,133,603 + ABIE53_004915 0.57 -0.7 1,133,603 + ABIE53_004915 0.57 +1.7 1,133,604 - ABIE53_004915 0.57 -1.0 1,133,604 - ABIE53_004915 0.57 +0.9 1,133,606 + ABIE53_004915 0.58 -0.1 1,133,606 + ABIE53_004915 0.58 -0.0 1,133,606 + ABIE53_004915 0.58 -0.1 1,133,607 - ABIE53_004915 0.58 -0.0 1,133,607 - ABIE53_004915 0.58 +1.9 1,133,611 + ABIE53_004915 0.59 +0.5 1,133,611 + ABIE53_004915 0.59 -1.0 1,133,611 + ABIE53_004915 0.59 +2.5 1,133,611 + ABIE53_004915 0.59 -0.1 1,133,611 + ABIE53_004915 0.59 -0.7 1,133,612 - ABIE53_004915 0.59 -1.0 1,133,706 + ABIE53_004915 0.82 +2.9 1,133,707 - ABIE53_004915 0.83 +1.6 1,133,707 - ABIE53_004915 0.83 -0.1 1,133,707 - ABIE53_004915 0.83 -0.0 1,133,707 - ABIE53_004915 0.83 +0.9 1,133,707 - ABIE53_004915 0.83 +1.2 1,133,707 - ABIE53_004915 0.83 -0.4 1,133,777 + +3.7 1,133,777 + +0.6 1,133,777 + +1.9 1,133,778 - +1.1 1,133,778 - +0.6 1,133,778 - +1.9 1,133,778 - -0.1 1,133,778 - +0.5 1,133,778 - -0.6 1,133,778 - -0.4 1,133,887 + +2.7 1,133,888 - -1.0 1,133,888 - -0.5 1,134,071 + -0.7 1,134,071 + +0.5 1,134,071 + +0.5 1,134,071 + +0.5 1,134,072 - -0.7 1,134,072 - -0.7 1,134,119 + +2.5 1,134,120 - +0.7 1,134,120 - +0.1 1,134,120 - +0.9 1,134,120 - +0.4 1,134,120 - -1.4 1,134,120 - -1.1 1,134,250 - +0.2 1,134,250 - +1.3 1,134,265 + -1.7 1,134,265 + +0.9 1,134,265 + +0.9 1,134,265 + -2.4 1,134,265 + +0.7 1,134,265 + -0.1 1,134,265 + -0.3 1,134,266 - -0.7 1,134,272 + +0.6 1,134,272 + +0.5 1,134,272 + -1.4 1,134,272 + +1.9 1,134,273 - +0.9 1,134,273 - -0.2 1,134,273 - -0.2 1,134,273 - +0.4 1,134,276 + -1.2 1,134,276 + -0.3 1,134,276 + -0.9 1,134,277 - +0.9 1,134,277 - -2.4 1,134,277 - -0.7 1,134,277 - +0.3 1,134,333 - +0.9 1,134,494 + ABIE53_004917 0.15 -0.8 1,134,494 + ABIE53_004917 0.15 +1.6 1,134,494 + ABIE53_004917 0.15 -0.9 1,134,495 - ABIE53_004917 0.15 -0.1 1,134,495 - ABIE53_004917 0.15 -0.4 1,134,542 + ABIE53_004917 0.23 +0.1 1,134,542 + ABIE53_004917 0.23 -1.7 1,134,543 - ABIE53_004917 0.23 +0.9 1,134,543 - ABIE53_004917 0.23 +0.6 1,134,543 - ABIE53_004917 0.23 +1.9 1,134,543 - ABIE53_004917 0.23 -0.9 1,134,587 + ABIE53_004917 0.30 +0.4 1,134,587 + ABIE53_004917 0.30 +0.5 1,134,587 + ABIE53_004917 0.30 -0.2 1,134,587 + ABIE53_004917 0.30 -0.6 1,134,588 - ABIE53_004917 0.30 +0.9 1,134,588 - ABIE53_004917 0.30 +0.9 1,134,588 - ABIE53_004917 0.30 -0.6 1,134,630 + ABIE53_004917 0.37 +2.9 1,134,638 + ABIE53_004917 0.38 +0.9 1,134,641 + ABIE53_004917 0.38 +0.9 1,134,642 - ABIE53_004917 0.39 -3.0 1,134,776 + ABIE53_004917 0.60 +3.5 1,134,776 + ABIE53_004917 0.60 +1.9 1,134,776 + ABIE53_004917 0.60 -1.7 1,134,776 + ABIE53_004917 0.60 +0.9 1,134,776 + ABIE53_004917 0.60 -0.4 1,134,777 - ABIE53_004917 0.60 +1.5 1,134,777 - ABIE53_004917 0.60 +0.4 1,134,777 - ABIE53_004917 0.60 +1.5 1,134,777 - ABIE53_004917 0.60 +0.2 1,134,777 - ABIE53_004917 0.60 -0.3
Or see this region's nucleotide sequence