Experiment: BHIS with Oxytetracycline 0.00078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1812 and BT1813 are separated by 192 nucleotides BT1813 and BT1814 overlap by 14 nucleotides
BT1812: BT1812 - putative chaperone DnAJ (NCBI ptt file), at 2,273,834 to 2,274,799
BT1812
BT1813: BT1813 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 2,274,992 to 2,276,695
BT1813
BT1814: BT1814 - conserved hypothetical protein (NCBI ptt file), at 2,276,682 to 2,277,878
BT1814
Position (kb)
2274
2275
2276
2277 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2274.067 kb on + strand, within BT1812 at 2274.069 kb on + strand, within BT1812 at 2274.070 kb on - strand, within BT1812 at 2274.078 kb on - strand, within BT1812 at 2274.089 kb on + strand, within BT1812 at 2274.093 kb on + strand, within BT1812 at 2274.119 kb on + strand, within BT1812 at 2274.150 kb on + strand, within BT1812 at 2274.150 kb on + strand, within BT1812 at 2274.257 kb on - strand, within BT1812 at 2274.456 kb on + strand, within BT1812 at 2274.604 kb on - strand, within BT1812 at 2274.685 kb on + strand, within BT1812 at 2274.737 kb on + strand at 2274.738 kb on - strand at 2274.780 kb on + strand at 2274.781 kb on - strand at 2274.793 kb on - strand at 2274.793 kb on - strand at 2274.894 kb on - strand at 2274.900 kb on + strand at 2274.904 kb on - strand at 2274.914 kb on + strand at 2274.933 kb on + strand at 2274.958 kb on + strand at 2274.980 kb on - strand at 2274.987 kb on - strand at 2275.001 kb on - strand at 2275.088 kb on + strand at 2275.107 kb on + strand at 2275.143 kb on + strand at 2275.202 kb on + strand, within BT1813 at 2275.202 kb on + strand, within BT1813 at 2275.303 kb on - strand, within BT1813 at 2275.380 kb on + strand, within BT1813 at 2275.441 kb on + strand, within BT1813 at 2275.447 kb on + strand, within BT1813 at 2275.462 kb on + strand, within BT1813 at 2275.491 kb on + strand, within BT1813 at 2275.491 kb on + strand, within BT1813 at 2275.492 kb on - strand, within BT1813 at 2275.508 kb on + strand, within BT1813 at 2275.578 kb on + strand, within BT1813 at 2275.590 kb on - strand, within BT1813 at 2275.619 kb on + strand, within BT1813 at 2275.635 kb on - strand, within BT1813 at 2275.819 kb on - strand, within BT1813 at 2275.843 kb on + strand, within BT1813 at 2275.865 kb on + strand, within BT1813 at 2276.009 kb on + strand, within BT1813 at 2276.010 kb on - strand, within BT1813 at 2276.010 kb on - strand, within BT1813 at 2276.075 kb on - strand, within BT1813 at 2276.126 kb on - strand, within BT1813 at 2276.126 kb on - strand, within BT1813 at 2276.127 kb on + strand, within BT1813 at 2276.127 kb on + strand, within BT1813 at 2276.127 kb on + strand, within BT1813 at 2276.128 kb on - strand, within BT1813 at 2276.128 kb on - strand, within BT1813 at 2276.132 kb on + strand, within BT1813 at 2276.132 kb on + strand, within BT1813 at 2276.133 kb on - strand, within BT1813 at 2276.133 kb on - strand, within BT1813 at 2276.185 kb on - strand, within BT1813 at 2276.251 kb on + strand, within BT1813 at 2276.390 kb on + strand, within BT1813 at 2276.411 kb on + strand, within BT1813 at 2276.440 kb on - strand, within BT1813 at 2276.440 kb on - strand, within BT1813 at 2276.489 kb on - strand, within BT1813 at 2276.533 kb on - strand at 2276.536 kb on - strand at 2276.564 kb on + strand at 2276.746 kb on + strand at 2276.754 kb on + strand at 2276.883 kb on + strand, within BT1814 at 2276.884 kb on - strand, within BT1814 at 2276.885 kb on + strand, within BT1814 at 2276.901 kb on + strand, within BT1814 at 2276.924 kb on + strand, within BT1814 at 2277.007 kb on + strand, within BT1814 at 2277.064 kb on + strand, within BT1814 at 2277.067 kb on + strand, within BT1814 at 2277.067 kb on + strand, within BT1814 at 2277.086 kb on + strand, within BT1814 at 2277.215 kb on + strand, within BT1814 at 2277.222 kb on - strand, within BT1814 at 2277.276 kb on + strand, within BT1814 at 2277.278 kb on + strand, within BT1814 at 2277.278 kb on + strand, within BT1814 at 2277.368 kb on - strand, within BT1814 at 2277.399 kb on - strand, within BT1814 at 2277.509 kb on - strand, within BT1814 at 2277.521 kb on + strand, within BT1814 at 2277.521 kb on + strand, within BT1814 at 2277.522 kb on - strand, within BT1814 at 2277.586 kb on + strand, within BT1814 at 2277.620 kb on + strand, within BT1814 at 2277.663 kb on + strand, within BT1814
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Oxytetracycline 0.00078125 mM remove 2,274,067 + BT1812 0.24 -1.6 2,274,069 + BT1812 0.24 -3.3 2,274,070 - BT1812 0.24 -1.2 2,274,078 - BT1812 0.25 -1.6 2,274,089 + BT1812 0.26 -0.3 2,274,093 + BT1812 0.27 +0.0 2,274,119 + BT1812 0.30 -0.1 2,274,150 + BT1812 0.33 -0.4 2,274,150 + BT1812 0.33 -1.0 2,274,257 - BT1812 0.44 -2.6 2,274,456 + BT1812 0.64 -1.2 2,274,604 - BT1812 0.80 -2.2 2,274,685 + BT1812 0.88 -0.8 2,274,737 + -0.8 2,274,738 - +0.7 2,274,780 + +0.8 2,274,781 - +0.6 2,274,793 - -1.0 2,274,793 - -1.2 2,274,894 - -1.5 2,274,900 + -0.3 2,274,904 - -1.2 2,274,914 + -1.0 2,274,933 + -0.4 2,274,958 + -1.3 2,274,980 - +0.0 2,274,987 - -2.3 2,275,001 - -2.3 2,275,088 + +0.0 2,275,107 + +1.0 2,275,143 + -1.0 2,275,202 + BT1813 0.12 +0.2 2,275,202 + BT1813 0.12 +0.0 2,275,303 - BT1813 0.18 -2.0 2,275,380 + BT1813 0.23 -1.6 2,275,441 + BT1813 0.26 +0.7 2,275,447 + BT1813 0.27 +0.1 2,275,462 + BT1813 0.28 +0.7 2,275,491 + BT1813 0.29 +0.0 2,275,491 + BT1813 0.29 -0.3 2,275,492 - BT1813 0.29 -2.7 2,275,508 + BT1813 0.30 +0.0 2,275,578 + BT1813 0.34 -1.6 2,275,590 - BT1813 0.35 -1.8 2,275,619 + BT1813 0.37 +1.6 2,275,635 - BT1813 0.38 +0.0 2,275,819 - BT1813 0.49 -0.6 2,275,843 + BT1813 0.50 -2.6 2,275,865 + BT1813 0.51 +0.0 2,276,009 + BT1813 0.60 -0.5 2,276,010 - BT1813 0.60 -0.5 2,276,010 - BT1813 0.60 -0.9 2,276,075 - BT1813 0.64 -1.8 2,276,126 - BT1813 0.67 -2.3 2,276,126 - BT1813 0.67 +0.0 2,276,127 + BT1813 0.67 -2.0 2,276,127 + BT1813 0.67 -0.7 2,276,127 + BT1813 0.67 -1.0 2,276,128 - BT1813 0.67 -0.4 2,276,128 - BT1813 0.67 +0.5 2,276,132 + BT1813 0.67 +1.0 2,276,132 + BT1813 0.67 -0.6 2,276,133 - BT1813 0.67 -1.1 2,276,133 - BT1813 0.67 +1.3 2,276,185 - BT1813 0.70 -2.0 2,276,251 + BT1813 0.74 -2.0 2,276,390 + BT1813 0.82 +0.4 2,276,411 + BT1813 0.83 -0.3 2,276,440 - BT1813 0.85 +0.3 2,276,440 - BT1813 0.85 +1.0 2,276,489 - BT1813 0.88 -0.4 2,276,533 - +3.0 2,276,536 - +0.4 2,276,564 + -0.7 2,276,746 + -1.6 2,276,754 + +0.6 2,276,883 + BT1814 0.17 -1.8 2,276,884 - BT1814 0.17 +0.0 2,276,885 + BT1814 0.17 +2.0 2,276,901 + BT1814 0.18 +0.5 2,276,924 + BT1814 0.20 -1.0 2,277,007 + BT1814 0.27 +0.8 2,277,064 + BT1814 0.32 -0.2 2,277,067 + BT1814 0.32 +0.1 2,277,067 + BT1814 0.32 -0.1 2,277,086 + BT1814 0.34 +0.2 2,277,215 + BT1814 0.45 +1.2 2,277,222 - BT1814 0.45 +0.0 2,277,276 + BT1814 0.50 -0.1 2,277,278 + BT1814 0.50 -1.0 2,277,278 + BT1814 0.50 +0.6 2,277,368 - BT1814 0.57 -0.7 2,277,399 - BT1814 0.60 +1.0 2,277,509 - BT1814 0.69 -2.0 2,277,521 + BT1814 0.70 +0.0 2,277,521 + BT1814 0.70 -0.5 2,277,522 - BT1814 0.70 -0.8 2,277,586 + BT1814 0.76 +0.2 2,277,620 + BT1814 0.78 +0.5 2,277,663 + BT1814 0.82 -1.3
Or see this region's nucleotide sequence