Experiment: BHIS with Zinc Pyrithione 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4320 and BT4321 are separated by 82 nucleotides BT4321 and BT4322 are separated by 83 nucleotides BT4322 and BT4323 are separated by 80 nucleotides
BT4320: BT4320 - putative zinc protease (NCBI ptt file), at 5,689,383 to 5,692,223
BT4320
BT4321: BT4321 - 2-dehydro-3-deoxyphosphooctonate aldolase (NCBI ptt file), at 5,692,306 to 5,693,106
BT4321
BT4322: BT4322 - conserved hypothetical protein, with a diacylglycerol kinase catalytic domain (NCBI ptt file), at 5,693,190 to 5,694,116
BT4322
BT4323: BT4323 - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI ptt file), at 5,694,197 to 5,695,117
BT4323
Position (kb) 5693
5694
5695 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 5692.280 kb on + strand at 5693.127 kb on - strand at 5693.202 kb on - strand at 5693.585 kb on - strand, within BT4322 at 5693.585 kb on - strand, within BT4322 at 5693.755 kb on - strand, within BT4322 at 5693.795 kb on - strand, within BT4322 at 5693.928 kb on - strand, within BT4322 at 5693.992 kb on - strand, within BT4322 at 5694.143 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Zinc Pyrithione 0.025 mM remove 5,692,280 + -4.0 5,693,127 - -0.1 5,693,202 - +5.0 5,693,585 - BT4322 0.43 +3.0 5,693,585 - BT4322 0.43 +2.7 5,693,755 - BT4322 0.61 +2.9 5,693,795 - BT4322 0.65 +0.1 5,693,928 - BT4322 0.80 +0.5 5,693,992 - BT4322 0.87 +0.1 5,694,143 - +4.1
Or see this region's nucleotide sequence