Experiment: BHIS with Zinc Pyrithione 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1064 and BT1065 overlap by 20 nucleotides BT1065 and BT1066 are separated by 11 nucleotides BT1066 and BT1067 are separated by 14 nucleotides BT1067 and BT1068 are separated by 13 nucleotides BT1068 and BT1069 are separated by 359 nucleotides
BT1064: BT1064 - conserved hypothetical protein (NCBI ptt file), at 1,352,127 to 1,353,305
BT1064
BT1065: BT1065 - hypothetical protein (NCBI ptt file), at 1,353,286 to 1,353,648
BT1065
BT1066: BT1066 - hypothetical protein (NCBI ptt file), at 1,353,660 to 1,354,229
BT1066
BT1067: BT1067 - hypothetical protein (NCBI ptt file), at 1,354,244 to 1,356,070
BT1067
BT1068: BT1068 - hypothetical protein (NCBI ptt file), at 1,356,084 to 1,356,428
BT1068
BT1069: BT1069 - conserved hypothetical protein, putative transcriptional regulator (NCBI ptt file), at 1,356,788 to 1,357,330
BT1069
Position (kb)
1354
1355
1356
1357 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1353.250 kb on - strand at 1353.253 kb on + strand at 1353.253 kb on + strand at 1353.253 kb on + strand at 1353.321 kb on + strand at 1353.372 kb on - strand, within BT1065 at 1353.416 kb on + strand, within BT1065 at 1353.416 kb on + strand, within BT1065 at 1353.504 kb on - strand, within BT1065 at 1353.542 kb on - strand, within BT1065 at 1353.549 kb on + strand, within BT1065 at 1353.560 kb on + strand, within BT1065 at 1353.651 kb on + strand at 1353.657 kb on - strand at 1353.673 kb on + strand at 1353.709 kb on + strand at 1353.766 kb on + strand, within BT1066 at 1353.773 kb on + strand, within BT1066 at 1353.792 kb on + strand, within BT1066 at 1353.859 kb on + strand, within BT1066 at 1353.890 kb on + strand, within BT1066 at 1353.943 kb on + strand, within BT1066 at 1353.969 kb on - strand, within BT1066 at 1354.060 kb on + strand, within BT1066 at 1354.124 kb on + strand, within BT1066 at 1354.140 kb on + strand, within BT1066 at 1354.140 kb on + strand, within BT1066 at 1354.212 kb on + strand at 1354.302 kb on + strand at 1354.373 kb on + strand at 1354.387 kb on + strand at 1354.388 kb on - strand at 1354.388 kb on - strand at 1354.477 kb on + strand, within BT1067 at 1354.603 kb on + strand, within BT1067 at 1354.640 kb on + strand, within BT1067 at 1354.643 kb on - strand, within BT1067 at 1354.645 kb on - strand, within BT1067 at 1354.692 kb on + strand, within BT1067 at 1354.754 kb on + strand, within BT1067 at 1354.813 kb on + strand, within BT1067 at 1354.951 kb on + strand, within BT1067 at 1354.987 kb on + strand, within BT1067 at 1354.987 kb on + strand, within BT1067 at 1355.049 kb on + strand, within BT1067 at 1355.110 kb on + strand, within BT1067 at 1355.113 kb on - strand, within BT1067 at 1355.130 kb on - strand, within BT1067 at 1355.151 kb on + strand, within BT1067 at 1355.191 kb on + strand, within BT1067 at 1355.221 kb on + strand, within BT1067 at 1355.223 kb on + strand, within BT1067 at 1355.350 kb on + strand, within BT1067 at 1355.529 kb on + strand, within BT1067 at 1355.629 kb on + strand, within BT1067 at 1355.726 kb on + strand, within BT1067 at 1355.742 kb on + strand, within BT1067 at 1355.746 kb on + strand, within BT1067 at 1356.059 kb on - strand at 1356.059 kb on - strand at 1356.279 kb on - strand, within BT1068 at 1356.329 kb on + strand, within BT1068 at 1356.333 kb on - strand, within BT1068 at 1356.364 kb on - strand, within BT1068 at 1356.366 kb on + strand, within BT1068 at 1356.369 kb on - strand, within BT1068 at 1356.443 kb on + strand at 1356.471 kb on + strand at 1356.706 kb on + strand at 1356.758 kb on + strand at 1356.759 kb on - strand at 1356.763 kb on + strand at 1356.763 kb on + strand at 1356.763 kb on + strand at 1356.965 kb on + strand, within BT1069 at 1356.991 kb on + strand, within BT1069 at 1357.024 kb on + strand, within BT1069
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Zinc Pyrithione 0.025 mM remove 1,353,250 - +0.1 1,353,253 + -1.9 1,353,253 + +0.5 1,353,253 + -0.8 1,353,321 + -0.1 1,353,372 - BT1065 0.24 +0.1 1,353,416 + BT1065 0.36 +0.1 1,353,416 + BT1065 0.36 +0.1 1,353,504 - BT1065 0.60 -3.9 1,353,542 - BT1065 0.71 -1.5 1,353,549 + BT1065 0.72 -0.9 1,353,560 + BT1065 0.75 -1.3 1,353,651 + -1.9 1,353,657 - -1.0 1,353,673 + -0.7 1,353,709 + -2.5 1,353,766 + BT1066 0.19 -0.9 1,353,773 + BT1066 0.20 -1.0 1,353,792 + BT1066 0.23 +0.1 1,353,859 + BT1066 0.35 -0.9 1,353,890 + BT1066 0.40 +2.5 1,353,943 + BT1066 0.50 +0.1 1,353,969 - BT1066 0.54 -1.9 1,354,060 + BT1066 0.70 +0.5 1,354,124 + BT1066 0.81 -0.4 1,354,140 + BT1066 0.84 -0.4 1,354,140 + BT1066 0.84 -1.3 1,354,212 + +2.1 1,354,302 + -1.1 1,354,373 + -0.3 1,354,387 + -0.6 1,354,388 - -1.9 1,354,388 - -0.9 1,354,477 + BT1067 0.13 -1.5 1,354,603 + BT1067 0.20 +0.0 1,354,640 + BT1067 0.22 +1.0 1,354,643 - BT1067 0.22 -2.3 1,354,645 - BT1067 0.22 +0.0 1,354,692 + BT1067 0.25 -1.5 1,354,754 + BT1067 0.28 -0.4 1,354,813 + BT1067 0.31 -1.8 1,354,951 + BT1067 0.39 -1.2 1,354,987 + BT1067 0.41 -1.3 1,354,987 + BT1067 0.41 -2.8 1,355,049 + BT1067 0.44 -1.7 1,355,110 + BT1067 0.47 +0.5 1,355,113 - BT1067 0.48 +0.0 1,355,130 - BT1067 0.48 +0.0 1,355,151 + BT1067 0.50 -1.1 1,355,191 + BT1067 0.52 -2.5 1,355,221 + BT1067 0.53 -1.5 1,355,223 + BT1067 0.54 -0.5 1,355,350 + BT1067 0.61 -3.1 1,355,529 + BT1067 0.70 +1.0 1,355,629 + BT1067 0.76 -1.0 1,355,726 + BT1067 0.81 +1.3 1,355,742 + BT1067 0.82 -1.0 1,355,746 + BT1067 0.82 +0.6 1,356,059 - -1.0 1,356,059 - +2.0 1,356,279 - BT1068 0.57 +0.0 1,356,329 + BT1068 0.71 -1.1 1,356,333 - BT1068 0.72 -1.1 1,356,364 - BT1068 0.81 +0.1 1,356,366 + BT1068 0.82 -1.5 1,356,369 - BT1068 0.83 -1.0 1,356,443 + -0.4 1,356,471 + +0.0 1,356,706 + +0.6 1,356,758 + -1.0 1,356,759 - -0.5 1,356,763 + -3.3 1,356,763 + -1.3 1,356,763 + -1.2 1,356,965 + BT1069 0.33 +0.0 1,356,991 + BT1069 0.37 -0.7 1,357,024 + BT1069 0.43 -1.0
Or see this region's nucleotide sequence