Experiment: KB with Paeonol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2467 and Psyr_2468 overlap by 4 nucleotides Psyr_2468 and Psyr_2469 are separated by 14 nucleotides
Psyr_2467: Psyr_2467 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 2,858,815 to 2,860,764
_2467
Psyr_2468: Psyr_2468 - Enoyl-CoA hydratase/isomerase, at 2,860,761 to 2,861,636
_2468
Psyr_2469: Psyr_2469 - Propionyl-CoA carboxylase, at 2,861,651 to 2,863,258
_2469
Position (kb)
2860
2861
2862 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2859.848 kb on + strand, within Psyr_2467 at 2859.848 kb on + strand, within Psyr_2467 at 2859.848 kb on + strand, within Psyr_2467 at 2859.848 kb on + strand, within Psyr_2467 at 2859.849 kb on - strand, within Psyr_2467 at 2859.868 kb on + strand, within Psyr_2467 at 2859.869 kb on - strand, within Psyr_2467 at 2859.951 kb on - strand, within Psyr_2467 at 2860.085 kb on + strand, within Psyr_2467 at 2860.085 kb on + strand, within Psyr_2467 at 2860.148 kb on + strand, within Psyr_2467 at 2860.149 kb on - strand, within Psyr_2467 at 2860.149 kb on - strand, within Psyr_2467 at 2860.574 kb on + strand at 2860.574 kb on + strand at 2860.575 kb on - strand at 2860.575 kb on - strand at 2860.630 kb on - strand at 2861.141 kb on - strand, within Psyr_2468 at 2861.347 kb on + strand, within Psyr_2468 at 2861.347 kb on + strand, within Psyr_2468 at 2861.347 kb on + strand, within Psyr_2468 at 2861.348 kb on - strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.497 kb on - strand, within Psyr_2468 at 2861.497 kb on - strand, within Psyr_2468 at 2861.611 kb on - strand at 2861.678 kb on - strand at 2861.784 kb on + strand at 2861.805 kb on + strand at 2861.957 kb on + strand, within Psyr_2469 at 2861.958 kb on - strand, within Psyr_2469 at 2861.994 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.105 kb on + strand, within Psyr_2469 at 2862.106 kb on - strand, within Psyr_2469 at 2862.106 kb on - strand, within Psyr_2469 at 2862.350 kb on + strand, within Psyr_2469 at 2862.351 kb on - strand, within Psyr_2469 at 2862.351 kb on - strand, within Psyr_2469 at 2862.516 kb on + strand, within Psyr_2469
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Paeonol 10 mM remove 2,859,848 + Psyr_2467 0.53 +1.2 2,859,848 + Psyr_2467 0.53 +1.2 2,859,848 + Psyr_2467 0.53 +1.2 2,859,848 + Psyr_2467 0.53 +0.1 2,859,849 - Psyr_2467 0.53 -1.8 2,859,868 + Psyr_2467 0.54 -1.4 2,859,869 - Psyr_2467 0.54 -1.2 2,859,951 - Psyr_2467 0.58 -0.3 2,860,085 + Psyr_2467 0.65 -1.2 2,860,085 + Psyr_2467 0.65 +0.8 2,860,148 + Psyr_2467 0.68 -1.5 2,860,149 - Psyr_2467 0.68 -0.6 2,860,149 - Psyr_2467 0.68 +0.2 2,860,574 + +1.5 2,860,574 + +2.7 2,860,575 - +0.3 2,860,575 - +1.2 2,860,630 - -1.8 2,861,141 - Psyr_2468 0.43 -1.0 2,861,347 + Psyr_2468 0.67 -0.6 2,861,347 + Psyr_2468 0.67 +0.4 2,861,347 + Psyr_2468 0.67 -0.6 2,861,348 - Psyr_2468 0.67 -1.6 2,861,392 + Psyr_2468 0.72 -1.8 2,861,393 - Psyr_2468 0.72 -0.3 2,861,393 - Psyr_2468 0.72 -0.4 2,861,497 - Psyr_2468 0.84 -1.4 2,861,497 - Psyr_2468 0.84 -1.7 2,861,611 - +2.7 2,861,678 - +0.4 2,861,784 + +0.4 2,861,805 + +0.9 2,861,957 + Psyr_2469 0.19 +0.0 2,861,958 - Psyr_2469 0.19 +1.4 2,861,994 + Psyr_2469 0.21 +0.4 2,862,105 + Psyr_2469 0.28 -0.0 2,862,105 + Psyr_2469 0.28 +0.8 2,862,105 + Psyr_2469 0.28 +1.0 2,862,106 - Psyr_2469 0.28 +0.4 2,862,106 - Psyr_2469 0.28 -2.4 2,862,350 + Psyr_2469 0.43 +1.0 2,862,351 - Psyr_2469 0.44 -0.1 2,862,351 - Psyr_2469 0.44 +2.7 2,862,516 + Psyr_2469 0.54 -1.3
Or see this region's nucleotide sequence