Experiment: KB with Paeonol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1740 and Psyr_1741 are separated by 12 nucleotides Psyr_1741 and Psyr_1742 are separated by 84 nucleotides Psyr_1742 and Psyr_1743 are separated by 64 nucleotides
Psyr_1740: Psyr_1740 - carbohydrate ABC transporter membrane protein 2, CUT1 family, at 1,977,203 to 1,978,003
_1740
Psyr_1741: Psyr_1741 - conserved hypothetical protein, at 1,978,016 to 1,978,288
_1741
Psyr_1742: Psyr_1742 - carbohydrate ABC transporter substrate-binding protein, CUT1 family, at 1,978,373 to 1,980,145
_1742
Psyr_1743: Psyr_1743 - methenyltetrahydrofolate cyclohydrolase / 5,10-methylenetetrahydrofolate dehydrogenase (NADP+), at 1,980,210 to 1,981,064
_1743
Position (kb)
1978
1979
1980
1981 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1977.405 kb on - strand, within Psyr_1740 at 1977.407 kb on + strand, within Psyr_1740 at 1977.407 kb on + strand, within Psyr_1740 at 1977.407 kb on + strand, within Psyr_1740 at 1977.407 kb on + strand, within Psyr_1740 at 1977.407 kb on + strand, within Psyr_1740 at 1977.408 kb on - strand, within Psyr_1740 at 1977.408 kb on - strand, within Psyr_1740 at 1977.566 kb on + strand, within Psyr_1740 at 1977.567 kb on - strand, within Psyr_1740 at 1977.567 kb on - strand, within Psyr_1740 at 1977.583 kb on - strand, within Psyr_1740 at 1977.583 kb on - strand, within Psyr_1740 at 1977.698 kb on + strand, within Psyr_1740 at 1977.698 kb on + strand, within Psyr_1740 at 1977.698 kb on + strand, within Psyr_1740 at 1977.699 kb on - strand, within Psyr_1740 at 1977.699 kb on - strand, within Psyr_1740 at 1978.190 kb on + strand, within Psyr_1741 at 1978.287 kb on - strand at 1978.347 kb on + strand at 1978.347 kb on + strand at 1978.347 kb on + strand at 1978.348 kb on - strand at 1978.372 kb on + strand at 1978.372 kb on + strand at 1978.373 kb on - strand at 1978.373 kb on - strand at 1978.373 kb on - strand at 1978.402 kb on + strand at 1978.402 kb on + strand at 1978.403 kb on - strand at 1978.403 kb on - strand at 1978.403 kb on - strand at 1978.467 kb on - strand at 1978.625 kb on + strand, within Psyr_1742 at 1978.626 kb on - strand, within Psyr_1742 at 1978.752 kb on - strand, within Psyr_1742 at 1978.901 kb on - strand, within Psyr_1742 at 1978.925 kb on + strand, within Psyr_1742 at 1978.926 kb on - strand, within Psyr_1742 at 1979.018 kb on + strand, within Psyr_1742 at 1979.018 kb on + strand, within Psyr_1742 at 1979.018 kb on + strand, within Psyr_1742 at 1979.019 kb on - strand, within Psyr_1742 at 1979.019 kb on - strand, within Psyr_1742 at 1979.019 kb on - strand, within Psyr_1742 at 1979.058 kb on - strand, within Psyr_1742 at 1979.132 kb on + strand, within Psyr_1742 at 1979.333 kb on + strand, within Psyr_1742 at 1979.334 kb on - strand, within Psyr_1742 at 1979.334 kb on - strand, within Psyr_1742 at 1979.334 kb on - strand, within Psyr_1742 at 1979.334 kb on - strand, within Psyr_1742 at 1979.355 kb on - strand, within Psyr_1742 at 1979.441 kb on + strand, within Psyr_1742 at 1979.441 kb on + strand, within Psyr_1742 at 1979.636 kb on + strand, within Psyr_1742 at 1979.636 kb on + strand, within Psyr_1742 at 1979.637 kb on - strand, within Psyr_1742 at 1979.637 kb on - strand, within Psyr_1742 at 1979.772 kb on - strand, within Psyr_1742 at 1979.992 kb on + strand at 1979.993 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Paeonol 10 mM remove 1,977,405 - Psyr_1740 0.25 +0.1 1,977,407 + Psyr_1740 0.25 +0.6 1,977,407 + Psyr_1740 0.25 -0.7 1,977,407 + Psyr_1740 0.25 -0.9 1,977,407 + Psyr_1740 0.25 +0.1 1,977,407 + Psyr_1740 0.25 +1.6 1,977,408 - Psyr_1740 0.26 -0.5 1,977,408 - Psyr_1740 0.26 +0.4 1,977,566 + Psyr_1740 0.45 -0.4 1,977,567 - Psyr_1740 0.45 +1.5 1,977,567 - Psyr_1740 0.45 +0.8 1,977,583 - Psyr_1740 0.47 -0.6 1,977,583 - Psyr_1740 0.47 +0.8 1,977,698 + Psyr_1740 0.62 +0.3 1,977,698 + Psyr_1740 0.62 -1.5 1,977,698 + Psyr_1740 0.62 -2.4 1,977,699 - Psyr_1740 0.62 +1.8 1,977,699 - Psyr_1740 0.62 +1.8 1,978,190 + Psyr_1741 0.64 +1.2 1,978,287 - +1.7 1,978,347 + -0.1 1,978,347 + +0.5 1,978,347 + +1.2 1,978,348 - -1.2 1,978,372 + -1.4 1,978,372 + +2.6 1,978,373 - -0.1 1,978,373 - -0.7 1,978,373 - -0.5 1,978,402 + +0.3 1,978,402 + +0.3 1,978,403 - +0.3 1,978,403 - -0.1 1,978,403 - -0.2 1,978,467 - -0.1 1,978,625 + Psyr_1742 0.14 +1.1 1,978,626 - Psyr_1742 0.14 +2.1 1,978,752 - Psyr_1742 0.21 +2.1 1,978,901 - Psyr_1742 0.30 +0.2 1,978,925 + Psyr_1742 0.31 +0.6 1,978,926 - Psyr_1742 0.31 +0.7 1,979,018 + Psyr_1742 0.36 +1.4 1,979,018 + Psyr_1742 0.36 +1.6 1,979,018 + Psyr_1742 0.36 -0.4 1,979,019 - Psyr_1742 0.36 -0.6 1,979,019 - Psyr_1742 0.36 +1.1 1,979,019 - Psyr_1742 0.36 -1.8 1,979,058 - Psyr_1742 0.39 -0.4 1,979,132 + Psyr_1742 0.43 +0.4 1,979,333 + Psyr_1742 0.54 -0.3 1,979,334 - Psyr_1742 0.54 +1.9 1,979,334 - Psyr_1742 0.54 -0.1 1,979,334 - Psyr_1742 0.54 +0.8 1,979,334 - Psyr_1742 0.54 +1.3 1,979,355 - Psyr_1742 0.55 +0.1 1,979,441 + Psyr_1742 0.60 -0.1 1,979,441 + Psyr_1742 0.60 -0.5 1,979,636 + Psyr_1742 0.71 -0.2 1,979,636 + Psyr_1742 0.71 -0.8 1,979,637 - Psyr_1742 0.71 +2.5 1,979,637 - Psyr_1742 0.71 +0.1 1,979,772 - Psyr_1742 0.79 -0.3 1,979,992 + +1.9 1,979,993 - +0.1
Or see this region's nucleotide sequence