Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS00630 and AZOBR_RS00635 are separated by 134 nucleotides AZOBR_RS00635 and AZOBR_RS00640 are separated by 155 nucleotides AZOBR_RS00640 and AZOBR_RS00645 are separated by 20 nucleotides
AZOBR_RS00630: AZOBR_RS00630 - acetylglutamate kinase, at 127,733 to 128,146
_RS00630
AZOBR_RS00635: AZOBR_RS00635 - pterin-4-alpha-carbinolamine dehydratase, at 128,281 to 128,583
_RS00635
AZOBR_RS00640: AZOBR_RS00640 - pyrophosphorylase, at 128,739 to 129,566
_RS00640
AZOBR_RS00645: AZOBR_RS00645 - magnesium transporter, at 129,587 to 130,576
_RS00645
Position (kb) 128
129
130 Strain fitness (log2 ratio) -1
0
1
2 at 127.854 kb on + strand, within AZOBR_RS00630 at 127.930 kb on + strand, within AZOBR_RS00630 at 127.930 kb on + strand, within AZOBR_RS00630 at 128.146 kb on + strand at 128.147 kb on - strand at 128.158 kb on + strand at 128.159 kb on - strand at 128.159 kb on - strand at 128.246 kb on - strand at 128.377 kb on + strand, within AZOBR_RS00635 at 128.648 kb on - strand at 128.648 kb on - strand at 128.650 kb on - strand at 128.828 kb on - strand, within AZOBR_RS00640 at 129.588 kb on + strand at 129.588 kb on + strand at 130.356 kb on + strand, within AZOBR_RS00645 at 130.356 kb on + strand, within AZOBR_RS00645 at 130.356 kb on + strand, within AZOBR_RS00645 at 130.357 kb on - strand, within AZOBR_RS00645 at 130.357 kb on - strand, within AZOBR_RS00645 at 130.378 kb on - strand, within AZOBR_RS00645
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 127,854 + AZOBR_RS00630 0.29 +1.5 127,930 + AZOBR_RS00630 0.48 -1.3 127,930 + AZOBR_RS00630 0.48 -0.3 128,146 + +1.5 128,147 - -0.7 128,158 + -0.4 128,159 - -1.2 128,159 - +1.5 128,246 - -1.7 128,377 + AZOBR_RS00635 0.32 +0.4 128,648 - -0.3 128,648 - -0.0 128,650 - -1.6 128,828 - AZOBR_RS00640 0.11 +1.0 129,588 + +2.5 129,588 + -1.6 130,356 + AZOBR_RS00645 0.78 +1.5 130,356 + AZOBR_RS00645 0.78 +0.5 130,356 + AZOBR_RS00645 0.78 -1.7 130,357 - AZOBR_RS00645 0.78 -0.4 130,357 - AZOBR_RS00645 0.78 -1.6 130,378 - AZOBR_RS00645 0.80 +0.5
Or see this region's nucleotide sequence